Press Release

A mass spectrometric method for in-depth profiling of phosphoinositide regioisomers and their disease-associated regulation

Published: January 13, 2022

Abstract

Phosphoinositides are a family of membrane lipids essential for many biological and pathological processes. Due to the existence of multiple phosphoinositide regioisomers and their low intracellular concentrations, profiling these lipids and linking a specific acyl variant to a change in biological state have been difficult. To enable the comprehensive analysis of phosphoinositide phosphorylation status and acyl chain identity, we develop PRMC-MS (Phosphoinositide Regioisomer Measurement by Chiral column chromatography and Mass Spectrometry). Using this method, we reveal a severe skewing in acyl chains in phosphoinositides in Pten-deficient prostate cancer tissues, extracellular mobilization of phosphoinositides upon expression of oncogenic PIK3CA, and a unique profile for exosomal phosphoinositides. Thus, our approach allows characterizing the dynamics of phosphoinositide acyl variants in intracellular and extracellular milieus.

Journal Article

JOURNAL Nature Communications

TITLE:A mass spectrometric method for in-depth profiling of phosphoinositide regioisomers and their disease-associated regulation

DOIhttps://doi.org/10.1038/s41467-021-27648-z

Correspondence to

Takehiko Sasaki, Professor
Junko Sasaki,Associate Professor

Department of Biochemical Pathophysiology,
Medical Research Institute,
Tokyo Medical and Dental University(TMDU)
E-mail:tsasaki.pip(at) mri.tmd.ac.jp
E-mail:jsaspip(at) mri.tmd.ac.jp


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