AKAPs-PKA disruptors increase AQP2 activity independently of vasopressin in a model of nephrogenic diabetes insipidus
Abstract
Congenital nephrogenic diabetes insipidus (NDI) is characterized by the inability of the kidney to concentrate urine. Congenital NDI is mainly caused by loss-of-function mutations in the vasopressin type 2 receptor (V2R), leading to impaired aquaporin-2 (AQP2) water channel activity. So far, treatment options of congenital NDI either by rescuing mutant V2R with chemical chaperones or by elevating cyclic adenosine monophosphate (cAMP) levels have failed to yield effective therapies. Here we show that inhibition of A-kinase anchoring proteins (AKAPs) binding to PKA increases PKA activity and activates AQP2 channels in cortical collecting duct cells. In vivo, the low molecular weight compound 3,3′-diamino-4,4′-dihydroxydiphenylmethane (FMP-API-1) and its derivatives increase AQP2 activity to the same extent as vasopressin, and increase urine osmolality in the context of V2R inhibition. We therefore suggest that FMP-API-1 may constitute a promising lead compound for the treatment of congenital NDI caused by V2R mutations.
Journal Article
JOURNAL:
Nature Communications
TITLE:
AKAPs-PKA disruptors increase AQP2 activity independently of vasopressin in a model of nephrogenic diabetes insipidus
DOI:
https://doi.org/10.1038/s41467-018-03771-2
Nature Communications
TITLE:
AKAPs-PKA disruptors increase AQP2 activity independently of vasopressin in a model of nephrogenic diabetes insipidus
DOI:
https://doi.org/10.1038/s41467-018-03771-2
Correspondence to
Shinichi UCHIDA, M.D., Ph.D.,Professor
Fumiaki ANDO, M.D., Ph.D.,Assistant Professor
Department of Nephrology,
Graduated School of Medical and Dental Sciences,
Tokyo Medical and Dental University(TMDU)
E-mail:suchida.kid(at)tmd.ac.jp
Fumiaki ANDO, M.D., Ph.D.,Assistant Professor
Department of Nephrology,
Graduated School of Medical and Dental Sciences,
Tokyo Medical and Dental University(TMDU)
E-mail:suchida.kid(at)tmd.ac.jp