HFD-induced hepatic lipid accumulation and inflammation are decreased in Factor D deficient mouse

HFD-induced hepatic lipid accumulation and inflammation are decreased in Factor D deficient mouse

Abstract

Excessive intake of fat causes accumulation of fat in liver, leading to non-alcoholic fatty liver disease (NAFLD). High-fat diet (HFD) upregulates the expression of Factor D, a complement pathway component, in the liver of mice. However, the functions of Factor D in liver are not well known. Therefore, the current study investigated the relationship between Factor D and hepatic lipid accumulation using CRISPR/Cas9-mediated Factor D knockout (FD-KO) mice. Factor D deficiency downregulated expression of genes related to fatty acid uptake and de novo lipogenesis in the liver. Furthermore, Factor D deficiency reduced the expression of inflammatory factors (Tnf and Ccl2) and fibrosis markers and decreased accumulation of F4/80-positive macrophages. These data suggest that the Factor D deficiency improved hepatic lipid accumulation and hepatic inflammation in HFD-fed mice.

Journal Article

JOURNAL:
Scientific Reports

TITLE:
HFD-induced hepatic lipid accumulation and inflammation are decreased in Factor D deficient
mouse

DOI:

https://doi.org/10.1038/s41598-020-74617-5

Correspondence to

Masayuki YOSHIDA, Professor

Department of Life Sciences and Bioethics,
Graduate School of Medical and Dental Sciences,
Tokyo Medical and Dental University(TMDU)
E-mail:masa.vasc(at)tmd.ac.jp

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