Analysis of the mouse Y chromosome by single molecule sequencing with Y chromosome enrichment.

Analysis of the mouse Y chromosome by single molecule sequencing with Y chromosome enrichment.

Abstract

Since human and mouse Y chromosomes contain repeated sequences, it is difficult to determine the precise sequences and analyze the function of individual Y chromosome genes. Therefore, the causes of many diseases and abnormalities related to Y chromosome genes, such as male infertility, remain unclear. In this study, to elucidate the mouse Y chromosome, we enriched the mouse Y chromosome using a fluorescence activated cell sorter (FACS) equipped with commonly used UV and blue 488 nm lasers and read the nucleotides using the Oxford Nanopore MinION long-read sequencer. This sequencing strategy allows us to cover the whole known region as well as the potential undetermined region of the Y chromosome. FACS-based chromosome enrichment and long-read sequencing are suitable for analysis of the Y chromosome sequences and may lead to further understanding of the physiological role of Y chromosome genes.

Journal Article

JOURNAL:
Frontiers in Genetics

TITLE:
Analysis of the mouse Y chromosome by single molecule sequencing with Y chromosome enrichment.

DOI:
https://doi.org/10.3389/fgene.2020.00406

Correspondence to

Hiroshi ASAHARA, MD, Ph.D., Professor
Department of Systems BioMedicine,
Graduate School of Medical and Dental Sciences,
Tokyo Medical and Dental University(TMDU)
E-mail:asahara.syst(at)tmd.ac.jp


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