1. Alborzian Deh Sheikh, A, Akatsu, C., Abdu-Allah, H. H. M., Suganuma, Y., Imamura, A., Ando, H., Takematsu, H., Ishida, H. and Tsubata, T. (2021): The protein tyrosine phosphatase SHP-1 (PTPN-6) but not CD45 (PTPR-C) is essential for the ligand-mediated regulation of CD22 in BCR-ligated B cells. J. Immunol. 206: 2544-2551.

Ballet, R., Brennan, M., Brandl, C., Feng, N., Berri, J., Cheng, J., Ocón, B., Alborzian Deh Sheikh, A., Marki, A., Bi,Y., Abram, C. L., Lowell, C. A., Tsubata, T., Greenberg, H. B., Macauley, M. S., Ley, K., Nitschke, L., Butcher. E. C. (2021) CD22-Shp1 phosphatase axis controls integrin β7 display and B cell function in mucosal immunity. Nat. Immunol:22:381-390

Tsugawa, N., Yamada, D., Watabe, T., Onizawa, M, Wang, S., Nemoto, Y., Oshima, S., Tsubata, T., Adachi, T., Karasuyama, H., Watanabe, M., Blumberg, R., Okamoto, R., and Nagaishi, T. (2021) CEACAM1 specifically suppresses B cell receptor signaling-mediated activation. Biochem. Biophys. Res. Commun. 535: 99-105.

Alborzian Deh Sheikh, A., Gomaa, S., Li, X., Routledge, M., Saigo, K., Numoto, N., Angata, T., Hitomi, Y., Takematsu, H., Tsuiji, M., Ito, N., Kusunoki, S. and Tsubata, T. (2021): A Guillain-Barré syndrome-associated SIGLEC10 rare variant impairs its recognition of gangliosides. J. Autoimmun. 116: 102571.

Tsubata, T. (2020): Involvement of reactive oxygen species (ROS) in BCR signaling as a second messenger. In “B Cells in Immunity and Tolerance” ed. by Ji-Yang Wang, Springer pp. 37-46.

Alborzian Deh Sheikh, A., Akatsu, C. and Tsubata, T. (2020): Identification of Siglec Cis-ligands by Proximity Labeling. In “Lectin Purification and Analysis: Methods and Protocols” ed. by Jun Hirabayashi, Springer pp. 75-83.

Hong, R., Lai, N., Xiong, E., Ouchida, R., Sun, J., Zhou, Y., Tang, Y., Hikida, M., Tsubata, T., Tagawa, M., Wang, Y. and J.-y. Wang (2020): Distinct roles of BCNP1 in B cell development and activation. Int. Immunol. 32: 17-26.

Xiong, E., Li, Y., Min, Q., Cui, C., Hong, R., Lai, N., Wang, Y., Sun, J., Matsumoto, R., Takahashi, D., Hase, K., Shinkura, R., Tsubata, T., Wang, J.-Y. (2019): MZB1 promotes the secretion of J chain-containing dimeric IgA and is critical for the suppression of gut inflammation. Proc. Natl. Acad. Sci. USA.116: 13480-13489.

Hong, R., Lai, N., Ouchida, R., Xiong, E., Zhou, Y., Hikida, M., Tsubata, T., Wang, Y. and Wang, J.-y. (2019): The B cell novel protein 1 regulates BCR signaling and B cell apoptosis. Eur. J. Immunol.49: 911-917.

Feng, Y.-y., Tang, M., Suzuki, M., Gunasekara, C., Anbe, Y., Hiraoka, Y., Liu. J., Grasberger, H., Ohkita, M., Matsumura, Y., Wang, J.-y. and Tsubata, T. (2019): Essential role of NADPH oxidase-dependent production of reactive oxygen species in maintenance of sustained B cell receptor signaling and B cell proliferation. J. Immunol. 202: 2546-2557.

Tsubata, T (2019): Development of Siglec Regulators. In “Glycoscience: Basic Science to Application” ed. by Naoyuki Taniguchi , Tamao Endo, Jun Hirabayashi and Shoko Nishihara, Springer (in press).

Tsubata, T. (2019): CD72 is a negative regulator of B cell responses to nuclear lupus self-antigens and development of SLE. Immune Netw. 19:e1.

Tsubata, T. (2018): Ligand recognition determines the role of inhibitory B cell co-receptors in the regulation of B cell homeostasis and autoimmunity. Front Immunol. 9: 2276.

Lino, A. C., Dang, V. D., Lampropoulou, V., Welle, A., Joedicke, J., Pohar, J., Simon, Q., Thalmensi, J., Baures, A., Fluehler, V., Sakwa, I., Stervbo, U., Ries, S., Jouneau, L., Boudinot., P., Tsubata, T. Adachi, T., Hutloff, A., Doener, T., Zimber-Strobl, U., de Vos, A. F., Dahlke, K., Loh, G., Komiotis, S., Goosmann, C., Weill, J.-C., Raynaud, C.-A., Kaufmann, S. H. E., Walter, J. and Fillatreau, S. (2018) : LAG-3 expression identifies immunosuppressive natural regulatory plasma cells. Immunity. 49: 120-133.

Li, S., Liu, J., Min, Q., Ikawa, T., Yasuda, S., Yang, Y., Wang, Y.-Q., Tsubata, T., Zhao, Y. and Wang, J.-Y. (2018): Kelch-like protein 14 promotes B-1a but suppresses B-1b cell development. Int. Immunol. 30: 311-318.

Matsubara, N., Imamura, A., Yonemizu, T., Akatsu, C., Yang, H., Ueki, A., Watanabe, N., Abdu-Allah, H., Numoto, N., Takematsu, H., Kitazume, S., Tedder, T. F., Marth, J. D., Ito, N., Ando, H., Ishida, H., Kiso, M. and Tsubata, T. (2018): CD22-binding synthetic sialosides regulate B lymphocyte proliferation through CD22 ligand-dependent and independent pathways, and enhance antibody production in mice. Front. Immunol. 9: 820.

Liu, J., Zhu, H., Qian, J., Xiong, E., Zhang, L., Chu, Y., Kubagawa, H., Tsubata, T. and Wang, J.-Y. (2018): FcµR promotes the survival and activation of marginal zone B cells and protects mice against bacterial sepsis. Front. Immunol. 9: 160.

Alborzian Deh Sheikh, A., Akatsu, C., Imamura, A., Abdu-Allah, H. H. M., Takematsu, H., Ando, H., Ishida, H. and Tsubata, T. (2018): Proximity labeling of cis-ligands of CD22/Siglec-2 reveals stepwise α2,6 sialic acid-dependent and -independent interactions. Biochem. Biophys. Res. Comm. 495: 854-859.

Tsubata, T. (2018): Negative regulation of B cell responses and self-tolerance to RNA-related lupus self-antigen. Proc. Jpn. Acad. Ser. B Phys. Biol. Sci. 94 (1) :35-44 .

Liu, J., Xiong, E., Zhu, H., Mori, H., Yasuda, S., Kinoshita, K., Tsubata, T. and Wang, J.-Y. (2017): Efficient induction of Ig gene hypermutation in ex vivo-activated primary B cells. J. Immunol. 199(9):3023-3030.

Tsubata, T (2017): B cell tolerance and autoimmunity. F1000Research 6 (F1000 Faculty Rev.): 391.

Akatsu, C., Shinagawa, K., Numoto, N., Liu, Z., Konuscan, A.U., Aslam, M., Phoon, S., Adachi, T., Furukawa, K., Ito, N. and Tsubata. T. (2016): CD72 negatively regulates B lymphocyte responses to the lupus-related endogenous Toll-like receptor 7 ligand Sm/RNP. J. Exp. Med. 213: 2691-2706.

Li, Y., Takahashi, Y., Fujii, S., Suzuki, A., Tsubata, T., Hase, K. and Wang, J.-Y.(2016): EAF2 mediates germinal center B cell apoptosis to suppress excessive immune responses and prevent autoimmunity. Nature Communications. 3;7:10836.

Tsubata, T. (2016): CD22 and CD72 are inhibitory receptors dominantly expressed in B lymphocytes and regulate systemic autoimmune diseases. Z. Rheumatol. 75: 86-89.

Muro. R., Nitta. T., Okada. T., Ideta. H., Tsubata. T. and Suzuki. H. (2015): The Ras GTPase-activating protein Rasal3 supports survival of naive T cells. PLoS ONE.10: e0119898.

Ouchida. R., Lu. Q., Liu. J., Li. Y., Chu. Y., Tsubata. T. and Wang, J.-Y. (2015): FcμR interacts and cooperates with the B cell receptor to promote B cell survival. J. Immunol. 194: 3096-3101.

Tsubata, T. (2014): Siglecs and B cell regulation. In: Taniguchi N., Endo T., Hart G., Seeberger P., Wong C. (Ed.) Glycoscience: Biology and Medicine: SpringerReference ( Springer-Verlag Berlin Heidelberg. 2014-04-01 06:48:34 UTC.

Kawai, Y., Ouchida, R., Yamasaki, S., Dragone, L., Tsubata, T. and Wang, J.-Y. (2014): LAPTM5 promotes lysosomal degradation of intracellular but not the cell surface CD3ζ. Immunol. Cell Biol. 92:527-534.

Naito-Matsui, Y., Takada, S., Kano, Y., Iyoda, T., Sugai, M, Shimizu, A., Inaba, K., Nitschke, L., Tsubata, T., Oka, S., Kozutsumi, Y. and Takematsu, H. (2014): Functional evaluation of activation-dependent alterations in the sialogylcan composition of T cells. J. Biol. Chem. 289: 1564-1579.

Aslam, M., Kishi, Y. and Tsubata, T. (2013): Excess CD40L does not rescue anti-DNA B cells from clonal anergy. F1000 Research 2:218.

Xu, M., Hou, R., Sato-Hayashizaki, A., Man, R., Zhu, C., Wakabayashi, C, Hirose, S., Adachi, T. and Tsubata, T. (2013): CD72c is a modifier gene that regulates Faslpr-induced autoimmune disease. J. Immunol. 190:5436-45.

Koni, P., Bolduc, A., Takezaki, M., Amtani, Y., Huang, L., Nutt S. L., Kamanaka, M., Flavell, R. A., Mellor A. L, Tsubata, T., Shimoda, M. (2013): Constitutively CD40-activated B cells regulate CD8 T cell inflammatory response by IL-10 induction. J. Immunol. 190: 3189-3196.

Maeno, E., Tsubata, T. and Okada, Y (2012): Apoptotic volume decrease (AVD) is independent of mitochondrial dysfunction and initiator caspase activation. Cells 1: 1156-1167.

Hitomi, Y., Adachi, T., Tsuchiya, N., Honda, Z.-I., Tokunaga, K and Tsubata, T. (2012): Human CD72 splicing isoform responsible for resistance to systemic lupus erythematosus regulates serum immunoglobulin level and is localized in endoplasmic resticulum. BMC Immunol. 13: 72.

Klionsky, D.J., Abdalla, F. C. Tsubata, T. et al. (2012): Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy 8: 1-100.

Kishi, Y., Higuchi, T., Phoon, S., Kamiya, K., Riemekasten, G., Akiyoshi, K., Weigert, M. and Tsubata, T. (2012): Apoptotic marginal zone deletion of anti-Sm/ribonucleoprotein B cells. Proc. Natl. Acad. Sci. USA. 109(20) : 7811-6.

Tsubata T.(2012): Role Of Inhibitory Bcr Co-Receptors In Immunity. Infect Disord Drug Targets. Mar 6.


Magesh, S., Ando, H., Tsubata, T., Ishida, H. and Kiso, M. (2011): High-affinity ligands of Siglec receptors and their therapeutic potentials. Current Medicinal Chemistry 18: 3537-3550.

Abdu-Allah, H. H. M, Watanabe, K., Kanie, O., Tsubata, T. Ishida, H. and Kiso, M. (2011). Design and synthesis of multivalent heterobifunctional CD22-ligand as a potential immunomodulator. Synthesis 18: 2968-2974.

Poe, J. C., Smith , S. H., Haas, K. M., Yanabe, K., Tsubata, T., Matsushita, T. and Tedder, T. F. (2011) Amplified B lymphocyte CD40 signaling drives regulatory B10 cell expansion in mice. PLoS ONE 6: e22464.

Abdu-Allah, H. H. M., Watanabe, K., Completo, G. C., Sadagopan, M., Hayashizaki, K., Takaku, C., Tamanaka, T., Takematsu, H., Kozutsumi, Y., Paulson, J.C., Tsubata, T., Ando, H., Ishida, H. and Kiso, M. (2011): CD22-antagonists with nanomolar potency: The synergistic effect of hydrophobic groups at C-2 and C-9 of sialic acid scaffold. Bioorg. Med. Chem. 19: 1966-1971.

Watanabe, K., Tsuchiya, Y., Kawaguchi, Y., Sawada, S.-i., Ayame, H., Akiyoshi, K. and Tsubata, T. (2011): The use of cationic nanogels to deliver proteins to myeloma cells and primary T lymphocytes that poorly express heparan sulfate. Biomaterials 32: 5900-5905.

Man, R.-Y., Onodera, T., Komatsu, E. and Tsubata, T. (2010): Augmented B Lymphocyte Response to Antigen in the Absence of Antigen-induced B Lymphocyte Signaling in an IgG-transgenic Mouse Line. PLoS One 5: 8815.

Ishiura, N., Nakashima, H., Watanabe, R., Kuwano, Y., Adachi, T., Takahashi, Y., Tsubata, T., Okochi, H., Tamaki, K., Tedder, T. F. and Fujimoto M. (2010): Differential phosphorylation of functional tyrosines in CD19 modulates B lymphocyte activation. Eur. J. Immunol. 40: 1192-1204.

Kishi, Y., Aiba, Y., Higuchi, T., Furukawa, K., Tokuhisa, T., Takemori, T. and Tsubata, T. (2010): Augmented antibody response with premature germinal center regression in CD40L-transgenic mice. J. Immunol. 185: 211-219.

Bolduc, A., Long, E., Stapler, D., Cascalho, M., Tsubata, T. Koni, P. A. and Shimoda, M. (2010): Constitutive CD40L expression on B cells prematurely terminates germinal center response and leads to augmented plasma cell production in T cell areas. J. Immunol. 185: 220-230.

Watanabe, K. and Tsubata, T. (2009): Autophagy connects antigen receptor signaling to costimulatory signaling in B lymphocytes. Autophagy.5: 108-110.

Toda, M., Hisano, R., Yurugi, H., Akita, K., Maruyama, K., Inoue, M., Adachi, T., Tsubata, T. and Nakada, H. (2009): Ligation of tumor-produced mucins to CD22 dramatically impairs splenic marginal zone B cells. Biochem J. 417:673-683.

Hou, R., Ohtsuji, M., Ohtsuji, N., Zhang, L., Adachi, T., Hirose, S. and Tsubata, T. (2009): The centromeric interval of chromosome 4 derived from C57BL/6 mice accelerates type 1 diabetes in NOD.CD72b congenic mice. Biochem. Biophys. Res. Comm. 380: 193-197.

Yanaba, K., Bouaziz, J.-D., Matsushita, T., Tsubata, T., and Tedder, T. F. (2009): The development and function of regulatory B cells expressing IL-10 requires antigen receptor diversity and TLR signals. J. Immunol. 182: 7459-7472.

Abdu-Allah, H. H. M., Watanabe, K., Hayashizaki, K., Iwayama, Y., Takematsu, H., Kozutsumi, Y., Tsubata, T., Ishida, H. and Kiso, M (2009): Synthesis of biotinylated sialoside to probe CD22-ligand interactions. Tetrahedron Lett. 50: 4488-4491.

Engels, N., König, L. M., Heemann, C., Lutz, J., Tsubata, T., Griep, S., Scharader, V. and Wienands, J. (2009): Recruitment of the cytoplasmic adaptor Grb2 to surface IgG and IgE provides antigen receptor-intrinsic costimulation to class-switched B cells. Nature Immunol. 10: 1018-1025.

Abdu-Allah, H. H. M., Watanabe, K., Hayashizaki, K., Takaku, C., Tamanaka, T., Takematsu, H., Kozutsumi, Y., Tsubata, T., Ishida, H. and Kiso, M. (2009): Potent small molecule mouse CD22 inhibitors: exploring the interaction of the residue at C-2 of sialic acid scaffold. Bioorg. Med. Chem. Lett. 19: 5573-5575

Yan, B.-C., Adachi, T. and Tsubata, T.(2008): ER stress is involved in B cell antigen receptor ligation-induced apoptosis. Biochem. Biophys. Res. Comm. 365:143-148.

Onodera, T., Poe, J. C., Tedder, T. F. and Tsubata, T. (2008): CD22 regulates time course of both B cell division and antibody response. J. Immunol. 180: 907-913.

Zhu, C., Fujimoto, M., Sato, M., Yanagisawa, T. and Tsubata, T. (2008): Novel binding site for SH2-containing protein tyrosine phosphatase-1 in CD22 activated by B Lymphocyte stimulation with antigen. J. Biol. Chem. 283: 1653-1659.

Adachi, T. and Tsubata, T. (2008): FRET-based Ca2+ measurement in B lymphocyte by flow cytometry and confocal microscopy. Biochem. Biophys. Res. Comm. 367: 377-382.

Watanabe, K., Ichinose, S., Hayashizaki, K. and Tsubata, T. (2008): Induction of autophagy by B cell antigen receptor stimulation and its inhibition by costimulation. Biochem. Biophys. Res. Comm. 374: 274-281

Tsubata, T. (2008). Siglec-2 is a key molecule for immune response. In “Experimental Glycoscience, Glycobiology” (ed by N. Taniguchi, A. Suzuki, Y. Ito, H. Narimatsu, T. Kawasaki and S. Hase) p. 167-170, Springer.

Abdu-Allah, H. H. M., Tamanaka, T., Yu, J., Lu, Z., Sadagopan, M., Adachi. T., Tsubata, T., Kelm, S., Ishida, H. and Kiso, M. (2008): Design, synthesis, and structure-activity relationships of novel series of sialosides as CD22-specific inhibitors. J. Med. Chem. 51: 6665-6681.

Sato, M., Adachi, T. and Tsubata, T. (2007): Augmentation of signaling through BCR containing IgE but not that containing IgA due to lack of CD22-mediated signal regulation. J. Immunol. 178: 2901-2907.

Yu, J., Sawada, T., Adachi, T., Gao, X., Takematsu, H., Kozutsumi, Y., Ishida, H., Kiso, M. and Tsubata, T. (2007): Synthetic glycan ligand excludes CD22 from antigen receptor-containing lipid rafts. Biochem. Biophys. Res. Comm. 360: 759-764

Adachi, T., Wienands, J., Tsubata, T. and Kurosaki, T. (2007): Interdomain A is crucial for ITAM-dependent and -independent regulation of Syk. Biochem. Biophys. Res. Comm. 364: 111-7

Devi, S. S., Hagiyama, H., Adachi, T., Miyasaka, N. and Tsubata, T. (2006): The tumor suppressor p53 is not required for antigen receptor-mediated apoptosis of B lymphocytes. Signal Transduction 6: 54-61.

Akagawa, M., Ito, S., Toyoda, K., Ishii, Y., Tatsuda, E., Shibata, T., Kawai, Y., Ishino, K., Kishi, Y., Adachi, T., Tsubata, T., Takasaki, Y., Hattori, N., Matsuda, T. and Uchida, K. (2006): Bispecific antibodies against modified protein and DNA with oxidized lipids. Proc. Natl. Acad. Sci. USA. 103: 6160-5.

Tsubata, T. (2006): B cell abnormality and systemic lupus erythematosus. APLAR J. Rheum. 9: 372-376

Tsubata, T. (2005): B cell abnormality and autoimmune disorders. Autoimmunity 38: 331-337.

Hirai, H., Adachi, T., and Tsubata, T. (2004): Involvement of cell cycle progression in survival signaling through CD40 in B lymphocyte line WEHI-231. Cell Death Differ. 11: 261-269.

Kawamura, T., Kanai, T., Dohi, T., Uraushihara, K., Totsuka, T., Iiyama, R., Taneda, C., Yamazaki, M., Nakamura, T., Higuchi, T., Aiba, Y., Tsubata, T. and Watanabe, M. (2004): Ectopic CD40 ligand expression on B cells trigger intestinal inflammation. J. Immunol. 172: 6388-6397.

Nitschke, L. and Tsubata, T. (2004): Molecular interactions regulate BCR signal inhibition by CD22 and CD72. Trends Immunol. 25: 543-550.

Suzuki, A, Kaisho, T., Ohishi, M., Tsukio-Yamaguchi, M., Tsubata, T., Koni, P. A., Sasaki, T., Mak, T. W., and Nakano, T. (2003): Critical roles of Pten in B cell homeostasis and immunoglobulin class switch recombination. J. Exp. Med. 197: 657-667.

Hokazono, Y., Adachi, T., Wabl, M., Tada, N., Amagasa, T., and Tsubata, T. (2003): Inhibitory Co-receptors Activated by Antigens but Not by Anti-immunoglobulin Heavy Chain Antibodies Install Requirement of Co-stimulation through CD40 for Survival and Proliferation of B cells. J. Immunol. 171: 1835-1843.

Higuchi, T., Aiba, Y., Nomura, T., Matsuda, J., Mochida, K., Suzuki, M., Kikutani, H., Honjo, T., Nishioka, K. and Tsubata T. (2002): Cutting Edge: Ectopic expression of CD40 ligand on B cells induces lupus-like autoimmune disease. J. Immunol. 168: 9-12.

Wakabayashi, C., Adachi, T., Wienands, J. and Tsubata, T. (2002): A distinct signaling pathway used by the IgG-containing B cell antigen receptor. Science. 298: 2392-2395.