Tsubata, T. (2020): Involvement of reactive oxygen species (ROS) in BCR signaling as a second messenger. In “B Cells in Immunity and Tolerance” ed. by Ji-Yang Wang, Springer pp. 37-46.

Alborzian Deh Sheikh, A., Akatsu, C. and Tsubata, T. (2020): Identification of Siglec Cis-ligands by Proximity Labeling. In “Lectin Purification and Analysis: Methods and Protocols” ed. by Jun Hirabayashi, Springer pp. 75-83.

Hong, R., Lai, N., Xiong, E., Ouchida, R., Sun, J., Zhou, Y., Tang, Y., Hikida, M., Tsubata, T., Tagawa, M., Wang, Y. and J.-y. Wang (2020): Distinct roles of BCNP1 in B cell development and activation. Int. Immunol. 32: 17-26.

Xiong, E., Li, Y., Min, Q., Cui, C., Hong, R., Lai, N., Wang, Y., Sun, J., Matsumoto, R., Takahashi, D., Hase, K., Shinkura, R., Tsubata, T., Wang, J.-Y. (2019): MZB1 promotes the secretion of J chain-containing dimeric IgA and is critical for the suppression of gut inflammation. Proc. Natl. Acad. Sci. USA.116: 13480-13489.

Hong, R., Lai, N., Ouchida, R., Xiong, E., Zhou, Y., Hikida, M., Tsubata, T., Wang, Y. and Wang, J.-y. (2019): The B cell novel protein 1 regulates BCR signaling and B cell apoptosis. Eur. J. Immunol.49: 911-917.

Feng, Y.-y., Tang, M., Suzuki, M., Gunasekara, C., Anbe, Y., Hiraoka, Y., Liu. J., Grasberger, H., Ohkita, M., Matsumura, Y., Wang, J.-y. and Tsubata, T. (2019): Essential role of NADPH oxidase-dependent production of reactive oxygen species in maintenance of sustained B cell receptor signaling and B cell proliferation. J. Immunol. 202: 2546-2557.

Tsubata, T (2019): Development of Siglec Regulators. In “Glycoscience: Basic Science to Application” ed. by Naoyuki Taniguchi , Tamao Endo, Jun Hirabayashi and Shoko Nishihara, Springer (in press).

Tsubata, T. (2019): CD72 is a negative regulator of B cell responses to nuclear lupus self-antigens and development of SLE. Immune Netw. 19:e1.

Tsubata, T. (2018): Ligand recognition determines the role of inhibitory B cell co-receptors in the regulation of B cell homeostasis and autoimmunity. Front Immunol. 9: 2276.

Lino, A. C., Dang, V. D., Lampropoulou, V., Welle, A., Joedicke, J., Pohar, J., Simon, Q., Thalmensi, J., Baures, A., Fluehler, V., Sakwa, I., Stervbo, U., Ries, S., Jouneau, L., Boudinot., P., Tsubata, T. Adachi, T., Hutloff, A., Doener, T., Zimber-Strobl, U., de Vos, A. F., Dahlke, K., Loh, G., Komiotis, S., Goosmann, C., Weill, J.-C., Raynaud, C.-A., Kaufmann, S. H. E., Walter, J. and Fillatreau, S. (2018) : LAG-3 expression identifies immunosuppressive natural regulatory plasma cells. Immunity. 49: 120-133.

Li, S., Liu, J., Min, Q., Ikawa, T., Yasuda, S., Yang, Y., Wang, Y.-Q., Tsubata, T., Zhao, Y. and Wang, J.-Y. (2018): Kelch-like protein 14 promotes B-1a but suppresses B-1b cell development. Int. Immunol. 30: 311-318.

Matsubara, N., Imamura, A., Yonemizu, T., Akatsu, C., Yang, H., Ueki, A., Watanabe, N., Abdu-Allah, H., Numoto, N., Takematsu, H., Kitazume, S., Tedder, T. F., Marth, J. D., Ito, N., Ando, H., Ishida, H., Kiso, M. and Tsubata, T. (2018): CD22-binding synthetic sialosides regulate B lymphocyte proliferation through CD22 ligand-dependent and independent pathways, and enhance antibody production in mice. Front. Immunol. 9: 820.

Liu, J., Zhu, H., Qian, J., Xiong, E., Zhang, L., Chu, Y., Kubagawa, H., Tsubata, T. and Wang, J.-Y. (2018): FcµR promotes the survival and activation of marginal zone B cells and protects mice against bacterial sepsis. Front. Immunol. 9: 160.

Alborzian Deh Sheikh, A., Akatsu, C., Imamura, A., Abdu-Allah, H. H. M., Takematsu, H., Ando, H., Ishida, H. and Tsubata, T. (2018): Proximity labeling of cis-ligands of CD22/Siglec-2 reveals stepwise α2,6 sialic acid-dependent and -independent interactions. Biochem. Biophys. Res. Comm. 495: 854-859.

Tsubata, T. (2018): Negative regulation of B cell responses and self-tolerance to RNA-related lupus self-antigen. Proc. Jpn. Acad. Ser. B Phys. Biol. Sci. 94 (1) :35-44 .

Liu, J., Xiong, E., Zhu, H., Mori, H., Yasuda, S., Kinoshita, K., Tsubata, T. and Wang, J.-Y. (2017): Efficient induction of Ig gene hypermutation in ex vivo-activated primary B cells. J. Immunol. 199(9):3023-3030.

Tsubata, T (2017): B cell tolerance and autoimmunity. F1000Research 6 (F1000 Faculty Rev.): 391.

Akatsu, C., Shinagawa, K., Numoto, N., Liu, Z., Konuscan, A.U., Aslam, M., Phoon, S., Adachi, T., Furukawa, K., Ito, N. and Tsubata. T. (2016): CD72 negatively regulates B lymphocyte responses to the lupus-related endogenous Toll-like receptor 7 ligand Sm/RNP. J. Exp. Med. 213: 2691-2706.

Li, Y., Takahashi, Y., Fujii, S., Suzuki, A., Tsubata, T., Hase, K. and Wang, J.-Y.(2016): EAF2 mediates germinal center B cell apoptosis to suppress excessive immune responses and prevent autoimmunity. Nature Communications. 3;7:10836.

Tsubata, T. (2016): CD22 and CD72 are inhibitory receptors dominantly expressed in B lymphocytes and regulate systemic autoimmune diseases. Z. Rheumatol. 75: 86-89.

Muro. R., Nitta. T., Okada. T., Ideta. H., Tsubata. T. and Suzuki. H. (2015): The Ras GTPase-activating protein Rasal3 supports survival of naive T cells. PLoS ONE.10: e0119898.

Ouchida. R., Lu. Q., Liu. J., Li. Y., Chu. Y., Tsubata. T. and Wang, J.-Y. (2015): FcμR interacts and cooperates with the B cell receptor to promote B cell survival. J. Immunol. 194: 3096-3101.

Tsubata, T. (2014): Siglecs and B cell regulation. In: Taniguchi N., Endo T., Hart G., Seeberger P., Wong C. (Ed.) Glycoscience: Biology and Medicine: SpringerReference (www.springerreference.com). Springer-Verlag Berlin Heidelberg. 2014-04-01 06:48:34 UTC.

Kawai, Y., Ouchida, R., Yamasaki, S., Dragone, L., Tsubata, T. and Wang, J.-Y. (2014): LAPTM5 promotes lysosomal degradation of intracellular but not the cell surface CD3ζ. Immunol. Cell Biol. 92:527-534.

Naito-Matsui, Y., Takada, S., Kano, Y., Iyoda, T., Sugai, M, Shimizu, A., Inaba, K., Nitschke, L., Tsubata, T., Oka, S., Kozutsumi, Y. and Takematsu, H. (2014): Functional evaluation of activation-dependent alterations in the sialogylcan composition of T cells. J. Biol. Chem. 289: 1564-1579.

Aslam, M., Kishi, Y. and Tsubata, T. (2013): Excess CD40L does not rescue anti-DNA B cells from clonal anergy. F1000 Research 2:218.

Xu, M., Hou, R., Sato-Hayashizaki, A., Man, R., Zhu, C., Wakabayashi, C, Hirose, S., Adachi, T. and Tsubata, T. (2013): CD72c is a modifier gene that regulates Faslpr-induced autoimmune disease. J. Immunol. 190:5436-45.

Koni, P., Bolduc, A., Takezaki, M., Amtani, Y., Huang, L., Nutt S. L., Kamanaka, M., Flavell, R. A., Mellor A. L, Tsubata, T., Shimoda, M. (2013): Constitutively CD40-activated B cells regulate CD8 T cell inflammatory response by IL-10 induction. J. Immunol. 190: 3189-3196.

Maeno, E., Tsubata, T. and Okada, Y (2012): Apoptotic volume decrease (AVD) is independent of mitochondrial dysfunction and initiator caspase activation. Cells 1: 1156-1167.

Hitomi, Y., Adachi, T., Tsuchiya, N., Honda, Z.-I., Tokunaga, K and Tsubata, T. (2012): Human CD72 splicing isoform responsible for resistance to systemic lupus erythematosus regulates serum immunoglobulin level and is localized in endoplasmic resticulum. BMC Immunol. 13: 72.

Klionsky, D.J., Abdalla, F. C. Tsubata, T. et al. (2012): Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy 8: 1-100.

Kishi, Y., Higuchi, T., Phoon, S., Kamiya, K., Riemekasten, G., Akiyoshi, K., Weigert, M. and Tsubata, T. (2012): Apoptotic marginal zone deletion of anti-Sm/ribonucleoprotein B cells. Proc. Natl. Acad. Sci. USA. 109(20) : 7811-6.

Tsubata T.(2012): Role Of Inhibitory Bcr Co-Receptors In Immunity. Infect Disord Drug Targets. Mar 6.


Magesh, S., Ando, H., Tsubata, T., Ishida, H. and Kiso, M. (2011): High-affinity ligands of Siglec receptors and their therapeutic potentials. Current Medicinal Chemistry 18: 3537-3550.

Abdu-Allah, H. H. M, Watanabe, K., Kanie, O., Tsubata, T. Ishida, H. and Kiso, M. (2011). Design and synthesis of multivalent heterobifunctional CD22-ligand as a potential immunomodulator. Synthesis 18: 2968-2974.

Poe, J. C., Smith , S. H., Haas, K. M., Yanabe, K., Tsubata, T., Matsushita, T. and Tedder, T. F. (2011) Amplified B lymphocyte CD40 signaling drives regulatory B10 cell expansion in mice. PLoS ONE 6: e22464.

Abdu-Allah, H. H. M., Watanabe, K., Completo, G. C., Sadagopan, M., Hayashizaki, K., Takaku, C., Tamanaka, T., Takematsu, H., Kozutsumi, Y., Paulson, J.C., Tsubata, T., Ando, H., Ishida, H. and Kiso, M. (2011): CD22-antagonists with nanomolar potency: The synergistic effect of hydrophobic groups at C-2 and C-9 of sialic acid scaffold. Bioorg. Med. Chem. 19: 1966-1971.

Watanabe, K., Tsuchiya, Y., Kawaguchi, Y., Sawada, S.-i., Ayame, H., Akiyoshi, K. and Tsubata, T. (2011): The use of cationic nanogels to deliver proteins to myeloma cells and primary T lymphocytes that poorly express heparan sulfate. Biomaterials 32: 5900-5905.

Man, R.-Y., Onodera, T., Komatsu, E. and Tsubata, T. (2010): Augmented B Lymphocyte Response to Antigen in the Absence of Antigen-induced B Lymphocyte Signaling in an IgG-transgenic Mouse Line. PLoS One 5: 8815.

Ishiura, N., Nakashima, H., Watanabe, R., Kuwano, Y., Adachi, T., Takahashi, Y., Tsubata, T., Okochi, H., Tamaki, K., Tedder, T. F. and Fujimoto M. (2010): Differential phosphorylation of functional tyrosines in CD19 modulates B lymphocyte activation. Eur. J. Immunol. 40: 1192-1204.

Kishi, Y., Aiba, Y., Higuchi, T., Furukawa, K., Tokuhisa, T., Takemori, T. and Tsubata, T. (2010): Augmented antibody response with premature germinal center regression in CD40L-transgenic mice. J. Immunol. 185: 211-219.

Bolduc, A., Long, E., Stapler, D., Cascalho, M., Tsubata, T. Koni, P. A. and Shimoda, M. (2010): Constitutive CD40L expression on B cells prematurely terminates germinal center response and leads to augmented plasma cell production in T cell areas. J. Immunol. 185: 220-230.

Watanabe, K. and Tsubata, T. (2009): Autophagy connects antigen receptor signaling to costimulatory signaling in B lymphocytes. Autophagy.5: 108-110.

Toda, M., Hisano, R., Yurugi, H., Akita, K., Maruyama, K., Inoue, M., Adachi, T., Tsubata, T. and Nakada, H. (2009): Ligation of tumor-produced mucins to CD22 dramatically impairs splenic marginal zone B cells. Biochem J. 417:673-683.

Hou, R., Ohtsuji, M., Ohtsuji, N., Zhang, L., Adachi, T., Hirose, S. and Tsubata, T. (2009): The centromeric interval of chromosome 4 derived from C57BL/6 mice accelerates type 1 diabetes in NOD.CD72b congenic mice. Biochem. Biophys. Res. Comm. 380: 193-197.

Yanaba, K., Bouaziz, J.-D., Matsushita, T., Tsubata, T., and Tedder, T. F. (2009): The development and function of regulatory B cells expressing IL-10 requires antigen receptor diversity and TLR signals. J. Immunol. 182: 7459-7472.

Abdu-Allah, H. H. M., Watanabe, K., Hayashizaki, K., Iwayama, Y., Takematsu, H., Kozutsumi, Y., Tsubata, T., Ishida, H. and Kiso, M (2009): Synthesis of biotinylated sialoside to probe CD22-ligand interactions. Tetrahedron Lett. 50: 4488-4491.

Engels, N., König, L. M., Heemann, C., Lutz, J., Tsubata, T., Griep, S., Scharader, V. and Wienands, J. (2009): Recruitment of the cytoplasmic adaptor Grb2 to surface IgG and IgE provides antigen receptor-intrinsic costimulation to class-switched B cells. Nature Immunol. 10: 1018-1025.

Abdu-Allah, H. H. M., Watanabe, K., Hayashizaki, K., Takaku, C., Tamanaka, T., Takematsu, H., Kozutsumi, Y., Tsubata, T., Ishida, H. and Kiso, M. (2009): Potent small molecule mouse CD22 inhibitors: exploring the interaction of the residue at C-2 of sialic acid scaffold. Bioorg. Med. Chem. Lett. 19: 5573-5575

Yan, B.-C., Adachi, T. and Tsubata, T.(2008): ER stress is involved in B cell antigen receptor ligation-induced apoptosis. Biochem. Biophys. Res. Comm. 365:143-148.

Onodera, T., Poe, J. C., Tedder, T. F. and Tsubata, T. (2008): CD22 regulates time course of both B cell division and antibody response. J. Immunol. 180: 907-913.

Zhu, C., Fujimoto, M., Sato, M., Yanagisawa, T. and Tsubata, T. (2008): Novel binding site for SH2-containing protein tyrosine phosphatase-1 in CD22 activated by B Lymphocyte stimulation with antigen. J. Biol. Chem. 283: 1653-1659.

Adachi, T. and Tsubata, T. (2008): FRET-based Ca2+ measurement in B lymphocyte by flow cytometry and confocal microscopy. Biochem. Biophys. Res. Comm. 367: 377-382.

Watanabe, K., Ichinose, S., Hayashizaki, K. and Tsubata, T. (2008): Induction of autophagy by B cell antigen receptor stimulation and its inhibition by costimulation. Biochem. Biophys. Res. Comm. 374: 274-281

Tsubata, T. (2008). Siglec-2 is a key molecule for immune response. In “Experimental Glycoscience, Glycobiology” (ed by N. Taniguchi, A. Suzuki, Y. Ito, H. Narimatsu, T. Kawasaki and S. Hase) p. 167-170, Springer.

Abdu-Allah, H. H. M., Tamanaka, T., Yu, J., Lu, Z., Sadagopan, M., Adachi. T., Tsubata, T., Kelm, S., Ishida, H. and Kiso, M. (2008): Design, synthesis, and structure-activity relationships of novel series of sialosides as CD22-specific inhibitors. J. Med. Chem. 51: 6665-6681.

Sato, M., Adachi, T. and Tsubata, T. (2007): Augmentation of signaling through BCR containing IgE but not that containing IgA due to lack of CD22-mediated signal regulation. J. Immunol. 178: 2901-2907.

Yu, J., Sawada, T., Adachi, T., Gao, X., Takematsu, H., Kozutsumi, Y., Ishida, H., Kiso, M. and Tsubata, T. (2007): Synthetic glycan ligand excludes CD22 from antigen receptor-containing lipid rafts. Biochem. Biophys. Res. Comm. 360: 759-764

Adachi, T., Wienands, J., Tsubata, T. and Kurosaki, T. (2007): Interdomain A is crucial for ITAM-dependent and -independent regulation of Syk. Biochem. Biophys. Res. Comm. 364: 111-7

Devi, S. S., Hagiyama, H., Adachi, T., Miyasaka, N. and Tsubata, T. (2006): The tumor suppressor p53 is not required for antigen receptor-mediated apoptosis of B lymphocytes. Signal Transduction 6: 54-61.

Akagawa, M., Ito, S., Toyoda, K., Ishii, Y., Tatsuda, E., Shibata, T., Kawai, Y., Ishino, K., Kishi, Y., Adachi, T., Tsubata, T., Takasaki, Y., Hattori, N., Matsuda, T. and Uchida, K. (2006): Bispecific antibodies against modified protein and DNA with oxidized lipids. Proc. Natl. Acad. Sci. USA. 103: 6160-5.

Tsubata, T. (2006): B cell abnormality and systemic lupus erythematosus. APLAR J. Rheum. 9: 372-376

Tsubata, T. (2005): B cell abnormality and autoimmune disorders. Autoimmunity 38: 331-337.

Hirai, H., Adachi, T., and Tsubata, T. (2004): Involvement of cell cycle progression in survival signaling through CD40 in B lymphocyte line WEHI-231. Cell Death Differ. 11: 261-269.

Kawamura, T., Kanai, T., Dohi, T., Uraushihara, K., Totsuka, T., Iiyama, R., Taneda, C., Yamazaki, M., Nakamura, T., Higuchi, T., Aiba, Y., Tsubata, T. and Watanabe, M. (2004): Ectopic CD40 ligand expression on B cells trigger intestinal inflammation. J. Immunol. 172: 6388-6397.

Nitschke, L. and Tsubata, T. (2004): Molecular interactions regulate BCR signal inhibition by CD22 and CD72. Trends Immunol. 25: 543-550.

Suzuki, A, Kaisho, T., Ohishi, M., Tsukio-Yamaguchi, M., Tsubata, T., Koni, P. A., Sasaki, T., Mak, T. W., and Nakano, T. (2003): Critical roles of Pten in B cell homeostasis and immunoglobulin class switch recombination. J. Exp. Med. 197: 657-667.

Hokazono, Y., Adachi, T., Wabl, M., Tada, N., Amagasa, T., and Tsubata, T. (2003): Inhibitory Co-receptors Activated by Antigens but Not by Anti-immunoglobulin Heavy Chain Antibodies Install Requirement of Co-stimulation through CD40 for Survival and Proliferation of B cells. J. Immunol. 171: 1835-1843.

Higuchi, T., Aiba, Y., Nomura, T., Matsuda, J., Mochida, K., Suzuki, M., Kikutani, H., Honjo, T., Nishioka, K. and Tsubata T. (2002): Cutting Edge: Ectopic expression of CD40 ligand on B cells induces lupus-like autoimmune disease. J. Immunol. 168: 9-12.

Wakabayashi, C., Adachi, T., Wienands, J. and Tsubata, T. (2002): A distinct signaling pathway used by the IgG-containing B cell antigen receptor. Science. 298: 2392-2395.