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2016-2019

2019
S. Nakamura, K. Irie, H. Tanaka, K. Nishikawa, H. Suzuki, A. Tamura, S. Tsukita, Y. Fujiyoshi (2019) Morphologic determinant of tight junctions revealed by claudin-3 structures. Nat. Commun.10,816→Full-text
2018
S. Nakamura, Y. Fujiyoshi, K. Irie (2018)Enhancement of the thermostability of mouse claudin-3 on complex formation with the carboxyl-terminal region of Clostridium perfringens enterotoxin improves crystal quality. Acta Crystallogr. F Struct. Biol. Commun. 74(Pt 3):150-155.→Abstract
K. Abe, K. Irie, H. Nakanishi, H. Suzuki, Y. Fujiyoshi (2018) Crystal structures of the gastric proton pump. Nature 556:214-218.→Abstract
K. Irie, Y. Haga, T. Shimomura, Y. Fujiyoshi (2018) Optimized expression and purification of NavAb provide the structural insight into the voltage dependence. FEBS Lett. 592:274-283.→Full-text
2017
W. Shihoya, T. Nishizawa, K. Yamashita, A. Inoue, K. Hirata, F.M.N. Kadji, A. Okuta, K. Tani, J. Aoki, Y. Fujiyoshi, T. Doi, O. Nureki (2017) X-ray structures of endothelin ETB receptor bound to clinical antagonist bosentan and its analog. Nat. Struct. Mol. Biol. 24:758-764.→Abstract
K. Abe, J. Shimokawa, M. Naito, K. Munson, O. Vagin, G. Sachs, H. Suzuki, K. Tani, Y. Fujiyoshi (2017) The cryo-EM structure of gastric H+,K+-ATPase with bound BYK99, a high-affinity member of K+-competitive, imidazo[1,2-a]pyridine inhibitors. Sci. Rep. 7:6632.→Full-text
H. Suzuki, K. Tani, Y. Fujiyoshi (2017) Crystal structures of claudins: insights into their intermolecular interactions. Ann. N. Y. Acad. Sci. 1397:25-34.→Full-text
2016
A. Oshima, K. Tani, Y. Fujiyoshi (2016) Atomic structure of the innexin-6 gap junction channel determined by cryo-EM. Nat. Commun. 7:13681.→Full-text
W. Shihoya, T. Nishizawa, A. Okuta, K. Tani, N. Dohmae, Y. Fujiyoshi, O. Nureki, T. Doi (2016) Activation mechanism of endothelin ETB receptor by endothelin-1. Nature 537:363-368.→Abstract
T. Shimomura, K. Irie, Y. Fujiyoshi (2016) Molecular determinants of prokaryotic voltage-gated sodium channels for recognition of local anesthetics. FEBS J. 283:2881-2895.→Abstract
K. Abe, Y. Fujiyoshi (2016) Cryo-electron microscopy for structure analyses of membrane proteins in the lipid bilayer. Curr. Opin. Struct. Biol. 39:71-78.→Abstract
A. Okuta, K. Tani, S. Nishimura, Y. Fujiyoshi, T. Doi (2016) Thermostabilization of the human endothelin type-B receptor. J. Mol. Biol. 428:2265-2274.→Abstract
A. Oshima, T. Matsuzawa, K. Murata, K. Tani, Y. Fujiyoshi (2016) Hexadecameric structure of an invertebrate gap junction channel. J. Mol. Biol. 428:1227-1236.→Full-text
H. Tanaka, Y. Yamamoto, H. Kashihara, Y. Yamazaki, K. Tani, Y. Fujiyoshi, K. Mineta, K. Takeuchi, A. Tamura, S. Tsukita (2016) Claudin-21 has a paracellular channel role at tight junctions. Mol. Cell Biol. 36:954-964.→Abstract
H. Nagura, T. Doi, Y. Fujiyoshi (2016) Characterization of physiologic phenotypes of dentate gyrus synapses of PDZ1/2 domain-deficient PSD-95 knockin mice. Eur. J. Neurosci. 43:618-625.→Abstract
A. Kamegawa, Y. Hiroaki, K. Tani, Y. Fujiyoshi (2016) Two-dimensional crystal structure of aquaporin-4 bound to the inhibitor acetazolamide. Microscopy (Oxf) 65:177-184.→Full-text
Y. Bando, K. Irie, T. Shimomura, H. Umeshima, Y. Kushida, M. Kengaku, Y. Fujiyoshi, T. Hirano, Y. Tagawa (2016) Control of Spontaneous Ca2+ Transients Is Critical for Neuronal Maturation in the Developing Neocortex. Cereb. Cortex. 26:106-117.→Abstract

2011-2015

2015
Y. Fujiyoshi (2015) Development of the field of structural physiology. Proc. Jpn. Acad. Ser. B. Phys. Biol. Sci. 91:447-468.→Full-text
F. Hauer, C. Gerle, N. Fischer, A. Oshima, K. Shinzawa Itoh, S. Shimada, K. Yokoyama, Y. Fujiyoshi, H. Stark (2015) GraDeR: Membrane Protein Complex Preparation for Single-Particle Cryo-EM. Structure 23:1769-1775.→Abstract
K. Minatohara, Y. Murata, Y. Fujiyoshi, T. Doi (2015) An Intracellular Domain with a Novel Sequence Regulates Cell Surface Expression and Synaptic Clustering of Leucine-Rich Repeat Transmembrane Proteins in Hippocampal Neurons. J Neurochem. 134:618-628.→Abstract
C. Jiko, K.M. Davies, K. Shinzawa-Itoh, K. Tani, S. Maeda, D.J. Mills, T. Tsukihara, Y. Fujiyoshi, W. Kuhlbrandt, C. Gerle (2015) Bovine F1Fo ATP synthase monomers bend the lipid bilayer in 2D membrane crystals. Elife. 4:e06119.→Full-text
Y. Saitoh, H. Suzuki, K. Tani, K. Nishikawa, K. Irie, Y. Ogura, A. Tamura, S. Tsukita, Y. Fujiyoshi (2015) Structural insight into tight junction disassembly by Clostridium perfringens enterotoxin. Science 347:775-778.→Abstract
H. Suzuki, K. Tani, A. Tamura, S. Tsukita, Y. Fujiyoshi (2015) Model for the architecture of claudin-based paracellular ion channels through tight junctions. J. Mol. Biol. 427:291-297.→Full-text
2014
K. Abe, K. Tani, Y. Fujiyoshi (2014) Systematic Comparison of Molecular Conformations of H+,K+-ATPase Reveals an Important Contribution of the A-M2 Linker for the Luminal Gating. J. Biol. Chem. 289:30590-30601.→Full-text
H. Suzuki, T. Nishizawa, K. Tani, Y. Yamazaki, A. Tamura, R. Ishitani, N. Dohmae, S. Tsukita, O. Nureki, Y. Fujiyoshi (2014) Crystal Structure of a Claudin Provides Insight into the Architecture of Tight Junctions. Science 344:304-307.→Abstract
D. Morito, K. Nishikawa, J. Hoseki, A. Kitamura, Y. Kotani, K. Kiso, M. Kinjo, Y. Fujiyoshi, K. Nagata (2014) Moyamoya disease-associated protein mysterin/RNF213 is a novel AAA+ ATPase, which dynamically changes its oligomeric state. Sci. Rep. 4:4442.→Full-text
K. Tani, Y. Fujiyoshi (2014) Water channel structures analysed by electron crystallography. Biochim. Biophys. Acta. 1840:1605-1613.→Abstract
2013
F. Yang, K. Abe, K. Tani, Y. Fujiyoshi (2013) Carbon sandwich preparation preserves quality of two-dimensional crystals for cryo-electron microscopy. Microscopy (Oxf) 62:597-606.→Full-text
C.J. Tsai, K. Tani, K. Irie, Y. Hiroaki, T. Shimomura, D.G. McMillan, G.M. Cook, G.F.X. Schertler, Y. Fujiyoshi, X.D. Li (2013) Two alternative conformations of a voltage-gated sodium channel. J. Mol. Biol. 425:4074-4088.→Full-text
H. Suzuki, Y. Ito, Y. Yamazaki, K. Mineta, M. Uji, K. Abe, K. Tani, Y. Fujiyoshi, S. Tsukita (2013) The four-transmembrane protein IP39 of Euglena forms strands by a trimeric unit repeat. Nat. Commun. 4:1766.→Full-text
K. Tani, C. P. Arthur, M. Tamakoshi, K. Yokoyama, K. Mitsuoka, Y. Fujiyoshi, C. Gerle (2013) Visualization of two distinct states of disassembly in the bacterial V-ATPase from Thermus thermophilus. Microscopy (Oxf) 62:467-474.→Full-text
M. Goda, Y. Fujiyoshi, M. Sugimoto, R. Fujii (2013) Novel Dichromatic Chromatophores in the Integument of the Mandarin Fish Synchiropus splendidus. Biol. Bull. 224:14-17.→Full-text
A. Oshima, T. Matsuzawa, K. Nishikawa, Y. Fujiyoshi (2013) Oligomeric structure and functional characterization of C. elegans innexin-6 gap junction channels. J. Biol. Chem. 288:10513-10521.→Full-text
K. Minatohara, S. H. Ichikawa, T. Seki, Y. Fujiyoshi, T. Doi (2013) Ligand binding of PDZ domains has various roles in the synaptic clustering of SAP102 and PSD-95. Neurosci. Lett. 533:44-49.→Abstract
2012
H. Nagura, Y. Ishikawa, K. Kobayashi, K. Takao, T. Tanaka, K. Nishikawa, H. Tamura, S. Shiosaka, H. Suzuki, T. Miyakawa, Y. Fujiyoshi, T. Doi (2012) Impaired synaptic clustering of postsynaptic density proteins and altered signal transmission in hippocampal neurons, and disrupted learning behavior in PDZ1 and PDZ2 ligand binding-deficient PSD-95 knockin mice. Mol Brain 5:43.→Full-text
K. Abe, K. Tani, T. Friedrich, Y. Fujiyoshi (2012) Cryo-EM structure of gastric H+,K+-ATPase with a single occupied cation-binding site. Proc. Natl. Acad. Sci. U.S.A. 109:18401-18406.→Full-text
N. Unwin, Y. Fujiyoshi (2012) Gating movement of acetylcholine receptor caught by plunge-freezing. J. Mol. Biol. 422:617-634.→Full-text
K. Irie, T. Shimomura, Y. Fujiyoshi (2012) The C-terminal helical bundle of the tetrameric prokaryotic sodium channel accelerates the inactivation rate. Nat. Commun. 3:793.→Full-text
2011
Y. Fujiyoshi (2011) Electron crystallography for structural and functional studies of membrane proteins. J. Electron Microsc. (Tokyo) 60:S149-159.→Full-text
F. Rico, A. Oshima, P. Hinterdorfer, Y. Fujiyoshi, S. Scheuring (2011) Two-Dimensional Kinetics of Inter-Connexin Interactions from Single-Molecule Force Spectroscopy. J. Mol. Biol. 412:72-79.→Abstract
S. Wakao, M. Kitada, Y. Kuroda, T. Shigemoto, D. Matsuse, H. Akashi, Y. Tanimura, K. Tsuchiyama, T. Kikuchi, M. Goda, T. Nakahata, Y. Fujiyoshi, M. Dezawa (2011) Multilineage-differentiating stress-enduring (Muse) cells are a primary source of induced pluripotent stem cells in human fibroblasts. Proc. Natl. Acad. Sci. U.S.A. 108:9875-9880.→Full-text
Y. Ohta, K. Nishikawa, Y. Hiroaki, Y. Fujiyoshi (2011) Electron tomographic analysis of gap junctions in lateral giant fibers of crayfish. J. Struct. Biol. 175:49-61.→Abstract
M. Goda, M. Ohata, H. Ikoma, Y. Fujiyoshi, M. Sugimoto, R. Fujii (2011) Integumental reddish-violet coloration due to novel dichromatic chromatophores in the teleost fish, Pseudochromis diadema. Pigment Cell Melanoma Res. 24:614-617.→Abstract
Y. Fujiyoshi (2011) Structural physiology based on electron crystallography. Protein Sci. 20:806-817.→Abstract
K. Yakata, K. Tani, Y. Fujiyoshi (2011) Water permeability and characterization of aquaporin-11. J. Struct. Biol. 174:315-320.→Abstract
K. Abe, K. Tani, Y. Fujiyoshi (2011) Conformational rearrangement of gastric H+,K+-ATPase induced by an acid suppressant. Nat. Commun. 2:155.→Full-text
T. Shimomura, K. Irie, H. Nagura, T. Imai, Y. Fujiyoshi (2011) Arrangement and mobility of the voltage sensor domain in prokaryotic voltage-gated sodium channels. J. Biol. Chem. 286:7409-7417.→Abstract
A. Oshima, K. Tani, M. M. Toloue, Y. Hiroaki, A. Smock, S. Inukai, A. Cone, B. J. Nicholson, G. E. Sosinsky, Y. Fujiyoshi. (2011) Asymmetric Configurations and N-terminal Rearrangements in Connexin26 Gap Junction Channels. J. Mol. Biol. 405:724-735.→Abstract

2000-2010

2010
A. D. Schenk, R. K. Hite, A. Engel, Y. Fujiyoshi, T. Walz. (2010) Electron crystallography and aquaporins. Methods Enzymol. 483:91-119.→Abstract
D. Matsuse, M. Kitada, M. Kohama, K. Nishikawa, H. Makinoshima, S. Wakao, Y. Fujiyoshi, T. Heike, T. Nakahata, H. Akutsu, A. Umezawa, H. Harigae, J.- Kira, M. Dezawa. (2010) Human umbilical cord-derived mesenchymal stromal cells differentiate into functional Schwann cells that sustain peripheral nerve regeneration. J. Neuropathol. Exp. Neurol. 69:973-985.→Abstract
T. Mitsuma, K. Tani, Y. Hiroaki, A. Kamegawa, H. Suzuki, H. Hibino, Y. Kurachi, Y. Fujiyoshi. (2010) Influence of the cytoplasmic domains of aquaporin-4 on water conduction and array formation. J. Mol. Biol. 402:669-681.→Abstract
H. Nagura, K. Irie, T. Imai, T. Shimomura, T. Hige, Y. Fujiyoshi. (2010) Evidence for lateral mobility of voltage sensors in prokaryotic voltage-gated sodium channels. Biochem. Biophys. Res. Commun. 399:341-346.→Abstract
Y. Kuroda, M. Kitada, S. Wakao, K. Nishikawa, Y. Tanimura, H. Makinoshima, M. Goda, H. Akashi, A. Inutsuka, A. Niwa, T. Shigemoto, Y. Nabeshima, T. Nakahata, Y.I. Nabeshima, Y. Fujiyoshi, M. Dezawa. (2010) Unique multipotent cells in adult human mesenchymal cell populations. Proc. Natl. Acad. Sci. U. S. A. 107:8639-8643.→Abstract
K. Abe, K. Tani, Y. Fujiyoshi. (2010) Structural and functional characterization of H+,K+-ATPase with bound fluorinated phosphate analogs. J. Struct. Biol. 170:60-68.→Abstract
K. Irie, K. Kitagawa, H. Nagura, T. Imai, T. Shimomura, Y. Fujiyoshi. (2010) Comparative study of the gating motif and C-type inactivation in prokaryotic voltage-gated sodium channels. J. Biol. Chem. 285:3685-3694.→Abstract
2009
A. Inutsuka, M. Goda, Y. Fujiyoshi. (2009) Calyculin A-induced neurite retraction is critically dependent on actomyosin activation but not on polymerization state of microtubules. Biochem. Biophys. Res. Commun. 390:1160-1166.→Abstract
K. Noma, K. Kimura, K. Minatohara, H. Nakashima, Y. Nagao, A. Mizoguchi, Y. Fujiyoshi. (2009) Triple N-Glycosylation in the Long S5-P Loop Regulates the Activation and Trafficking of the Kv12.2 Potassium Channel. J. Biol. Chem. 284:33139-33150.→Abstract
Y. Kuwahara, S. Unzai, T. Nagata, Y. Hiroaki, H. Yokoyama, I. Matsui, T. Ikegami, Y. Fujiyoshi, H. Hiroaki. (2009) Unusual thermal disassembly of the SPFH domain oligomer from Pyrococcus horikoshii. Biophys. J. 97:2034-2043.→Abstract
K. Tani, T. Mitsuma, Y. Hiroaki, A. Kamegawa, K. Nishikawa, Y. Tanimura, Y. Fujiyoshi. (2009) Mechanism of aquaporin-4's fast and highly selective water conduction and proton exclusion. J. Mol. Biol. 389:694-706.→Abstract
K. Abe, K. Tani, T. Nishizawa, Y. Fujiyoshi. (2009) Inter-subunit interaction of gastric H+,K+-ATPase prevents reverse reaction of the transport cycle. EMBO J. 28:1637-1643.→Abstract
S. Maeda, S. Nakagawa, M. Suga, E. Yamashita, A. Oshima, Y. Fujiyoshi, T. Tsukihara. (2009) Structure of the connexin 26 gap junction channel at 3.5 A resolution. Nature 458:597-602.→Abstract
Y. Tanimura, Y. Hiroaki, Y. Fujiyoshi. (2009) Acetazolamide reversibly inhibits water conduction by aquaporin-4. J. Struct. Biol. 166:16-21.→Abstract
K. Mio, T. Ogura, T. Yamamoto, Y. Hiroaki, Y. Fujiyoshi, Y. Kubo, C. Sato. (2009) Reconstruction of the P2X2 receptor reveals a vase-shaped structure with lateral tunnels above the membrane. Structure 17:266-275.→Abstract
T. Walz, Y. Fujiyoshi, A. Engel. (2009) The AQP structure and functional implications. Handb. Exp. Pharmacol. 190:31-56.→Abstract
2008
T. Sakisaka, Y. Yamamoto, S. Mochida, M. Nakamura, K. Nishikawa, H. Ishizaki, M. Okamoto-Tanaka, J. Miyoshi, Y. Fujiyoshi, T. Manabe, Y. Takai. (2008) Dual inhibition of SNARE complex formation by tomosyn ensures controlled neurotransmitter release. J. Cell. Biol. 183:323-337.→Abstract
K. Iwasaki, N. Miyazaki, L. Hammar, Y. Zhu, T. Omura, B. Wu, F. Sjoborg, K. Yonekura, K. Murata, K. Namba, D. L. Caspar, Y. Fujiyoshi, R. H. Cheng. (2008) Pleomorphic configuration of the trimeric capsid proteins of Rice dwarf virus that allows formation of both the outer capsid and tubular crystals. J. Mol. Biol. 383:252-265.→Abstract
Y. Fujiyoshi, N. Unwin. (2008) Electron crystallography of proteins in membranes. Curr. Opin. Struct. Biol. 18:587-592.→Abstract
A. Oshima, K. Tani, Y. Hiroaki, Y. Fujiyoshi, G. E. Sosinsky. (2008) Projection structure of a N-terminal deletion mutant of connexin 26 channel with decreased central pore density. Cell Commun. Adhes. 15:85-93.→Abstract
S. Hayakawa, M. Mori, A. Okuta, A. Kamegawa, Y. Fujiyoshi, Y. Yoshiyama, K. Mitsuoka, K. Ishibashi, S. Sasaki, T. Hattori, S. Kuwabara. (2008) Neuromyelitis optica and anti-aquaporin-4 antibodies measured by an enzyme-linked immunosorbent assay. J. Neuroimmunol. 196:181-187.→Abstract
H. Suzuki, M. Kurooka, Y. Hiroaki, Y. Fujiyoshi, Y. Kaneda. (2008) Sendai virus F glycoprotein induces IL-6 production in dendritic cells in a fusion-independent manner. FEBS Lett. 582:1325-1329.→Abstract
T. Nishizawa, K. Abe, K. Tani, Y. Fujiyoshi. (2008) Structural analysis of 2D crystals of gastric H+,K+-ATPase in different states of the transport cycle. J. Struct. Biol. 162:219-228.→Abstract
A. Engel, Y. Fujiyoshi, T. Gonen, T. Walz. (2008) Junction-forming aquaporins. Curr. Opin. Struct. Biol. 18:229-235.→Abstract
H. Suzuki, K. Nishikawa, Y. Hiroaki, Y. Fujiyoshi. (2008) Formation of aquaporin-4 arrays is inhibited by palmitoylation of N-terminal cysteine residues. Biochim. Biophys. Acta 1778:1181-1189.→Full-text
2007
M. Toei, C. Gerle, M. Nakano, K. Tani, N. Gyobu, M. Tamakoshi, N. Sone, M. Yoshida, Y. Fujiyoshi, K. Mitsuoka, K. Yokoyama. (2007) Dodecamer rotor ring defines H+/ATP ratio for ATP synthesis of prokaryotic V-ATPase from Thermus thermophilus. Proc. Natl. Acad. Sci. U. S. A. 104:20256-20261.→Abstract
T. Hirai, K. Mitsuoka, A. Kidera, Y. Fujiyoshi. (2007) Simulation of charge effects on density maps obtained by high-resolution electron crystallography. J. Electron. Microsc. (Tokyo) 56:131-140.→Abstract
K. Mio, T. Ogura, S. Kiyonaka, Y. Hiroaki, Y. Tanimura, Y. Fujiyoshi, Y. Mori, C. Sato. (2007) The TRPC3 channel has a large internal chamber surrounded by signal sensing antennas. J. Mol. Biol. 367:373-383.→Abstract
A. Oshima, K. Tani, Y. Hiroaki, Y. Fujiyoshi, G. E. Sosinsky. (2007) Three-dimensional structure of a human connexin26 gap junction channel reveals a plug in the vestibule. Proc. Natl. Acad. Sci. U. S. A. 104:10034-10039.→Abstract
K. Yakata, Y. Hiroaki, K. Ishibashi, E. Sohara, S. Sasaki, K. Mitsuoka, Y. Fujiyoshi. (2007) Aquaporin-11 containing a divergent NPA motif has normal water channel activity. Biochim. Biophys. Acta 1768:688-693.→Full-text
2006
T. Hige, Y. Fujiyoshi, T. Takahashi. (2006) Neurosteroid pregnenolone sulfate enhances glutamatergic synaptic transmission by facilitating presynaptic calcium currents at the calyx of Held of immature rats. Eur. J. Neurosci. 24:1955-1966.→Abstract
P. J. Holm, P. Bhakat, C. Jegerschold, N. Gyobu, K. Mitsuoka, Y. Fujiyoshi, R. Morgenstern, H. Hebert. (2006) Structural basis for detoxification and oxidative stress protection in membranes. J. Mol. Biol. 360:934-945.→Abstract
M. Nonaka, T. Doi, Y. Fujiyoshi, S. Takemoto-Kimura, H. Bito. (2006) Essential contribution of the ligand-binding beta B/beta C loop of PDZ1 and PDZ2 in the regulation of postsynaptic clustering, scaffolding, and localization of postsynaptic density-95. J. Neurosci. 26:763-774.→Abstract
C. Gerle, K. Tani, K. Yokoyama, M. Tamakoshi, M. Yoshida, Y. Fujiyoshi, K. Mitsuoka. (2006) Two-dimensional crystallization and analysis of projection images of intact Thermus thermophilus V-ATPase. J. Struct. Biol. 153:200-206.→Abstract
Y. Hiroaki, K. Tani, A. Kamegawa, N. Gyobu, K. Nishikawa, H. Suzuki, T. Walz, S. Sasaki, K. Mitsuoka, K. Kimura, A. Mizoguchi, Y. Fujiyoshi. (2006) Implications of the aquaporin-4 structure on array formation and cell adhesion. J. Mol. Biol. 355:628-639.→Abstract
2005
T. Gonen, Y. Cheng, P. Sliz, Y. Hiroaki, Y. Fujiyoshi, S. C. Harrison, T. Walz. (2005) Lipid-protein interactions in double-layered two-dimensional AQP0 crystals. Nature 438:633-638.→Abstract
T. Kodama, H. Imai, T. Doi, O. Chisaka, Y. Shichida, Y. Fujiyoshi. (2005) Expression and localization of an exogenous G protein-coupled receptor fused with the rhodopsin C-terminal sequence in the retinal rod cells of knockin mice. Exp. Eye Res. 80:859-869.→Abstract
K. Iwasaki, K. Mitsuoka, Y. Fujiyoshi, Y. Fujisawa, M. Kikuchi, K. Sekiguchi, T. Yamada. (2005) Electron tomography reveals diverse conformations of integrin αIIbβ3 in the active state. J. Struct. Biol. 150:259-267.→Abstract
Y. Murata, T. Doi, H. Taniguchi, Y. Fujiyoshi. (2005) Proteomic analysis revealed a novel synaptic proline-rich membrane protein (PRR7) associated with PSD-95 and NMDA receptor. Biochem. Biophys. Res. Commun. 327:183-191.→Abstract
2004
N. Gyobu, K. Tani, Y. Hiroaki, A. Kamegawa, K. Mitsuoka, Y. Fujiyoshi. (2004) Improved specimen preparation for cryo-electron microscopy using a symmetric carbon sandwich technique. J. Struct. Biol. 146:325-333.→Abstract
T. Yamaguchi, I. Arimoto-Tahara, Y. Fujiyoshi, T. Doi. (2004) Characterization and application of monoclonal antibodies against human endothelin B receptor expressed in insect cells. Biotechnol. Lett. 26:293-299.→Abstract
C. Sato, K. Hamada, T. Ogura, A. Miyazawa, K. Iwasaki, Y. Hiroaki, K. Tani, A. Terauchi, Y. Fujiyoshi, K. Mikoshiba. (2004) Inositol 1,4,5-trisphosphate receptor contains multiple cavities and L-shaped ligand-binding domains. J. Mol. Biol. 336:155-164.→Abstract
2003
A. Miyazawa, Y. Fujiyoshi, N. Unwin. (2003) Structure and gating mechanism of the acetylcholine receptor pore. Nature 423:949-955.→Abstract
T. Yamaguchi, Y. Murata, Y. Fujiyoshi, T. Doi. (2003) Regulated interaction of endothelin B receptor with caveolin-1. Eur. J. Biochem. 270:1816-1827.→Abstract
Y. Kyogoku, Y. Fujiyoshi, I. Shimada, H. Nakamura, T. Tsukihara, H. Akutsu, T. Odahara, T. Okada, N. Nomura. (2003) Structural genomics of membrane proteins. Acc. Chem. Res. 36:199-206.→Abstract
A. Oshima, T. Doi, K. Mitsuoka, S. Maeda, Y. Fujiyoshi. (2003) Roles of Met-34, Cys-64, and Arg-75 in the assembly of human connexin 26. Implication for key amino acid residues for channel formation and function. J. Biol. Chem. 278:1807-1816.→Abstract
2002
Y. Fujiyoshi, K. Mitsuoka, B. L. de Groot, A. Philippsen, H. Grubmuller, P. Agre, A. Engel. (2002) Structure and function of water channels. Curr. Opin. Struct. Biol. 12:509-515.→Abstract
Y. Hiroaki, K. Nishikawa, K. Mitsuoka, T. Tachibana, K. Sobue, T. Doi, Y. Fujiyoshi. (2002) A new technique to co-localise membrane proteins with Homer/vesl. Biochem. Biophys. Res. Commun. 295:756-765.→Abstract
P. Agre, L. S. King, M. Yasui, W. B. Guggino, O. P. Ottersen, Y. Fujiyoshi, A. Engel, S. Nielsen. (2002) Aquaporin water channels--from atomic structure to clinical medicine. J. Physiol. 542:3-16.→Full-text
N. Unwin, A. Miyazawa, J. Li, Y. Fujiyoshi. (2002) Activation of the nicotinic acetylcholine receptor involves a switch in conformation of the alpha subunits. J. Mol. Biol. 319:1165-1176.→Abstract
K. Irie, T. Nakatsu, K. Mitsuoka, A. Miyazawa, K. Sobue, Y. Hiroaki, T. Doi, Y. Fujiyoshi, H. Kato. (2002) Crystal structure of the Homer 1 family conserved region reveals the interaction between the EVH1 domain and own proline-rich motif. J. Mol. Biol. 318:1117-1126.→Abstract
T. Okada, Y. Fujiyoshi, M. Silow, J. Navarro, E. M. Landau, Y. Shichida. (2002) Functional role of internal water molecules in rhodopsin revealed by X-ray crystallography. Proc. Natl. Acad. Sci. U. S. A. 99:5982-5987.→Abstract
F. Imamura, S. Maeda, T. Doi, Y. Fujiyoshi. (2002) Ligand binding of the second PDZ domain regulates clustering of PSD-95 with the Kv1.4 potassium channel. J. Biol. Chem. 277:3640-3646.→Abstract
2001
H. Stahlberg, D. Fotiadis, S. Scheuring, H. Remigy, T. Braun, K. Mitsuoka, Y. Fujiyoshi, A. Engel. (2001) Two-dimensional crystals: a powerful approach to assess structure, function and dynamics of membrane proteins. FEBS Lett. 504:166-172.→Full-text
C. Sato, Y. Ueno, K. Asai, K. Takahashi, M. Sato, A. Engel, Y. Fujiyoshi. (2001) The voltage-sensitive sodium channel is a bell-shaped molecule with several cavities. Nature 409:1047-1051.→Abstract
2000
Y. Tahara, A. Oshima, T. Hirai, K. Mitsuoka, Y. Fujiyoshi, Y. Hayashi. (2000) The 11 A resolution projection map of Na+/K+-ATPase calculated by application of single particle analysis to two-dimensional crystal images. J. Electron. Microsc. (Tokyo.) 49:583-587.→Abstract
I. Schmidt-Krey, K. Mitsuoka, T. Hirai, K. Murata, Y. Cheng, Y. Fujiyoshi, R. Morgenstern, H. Hebert. (2000) The three-dimensional map of microsomal glutathione transferase 1 at 6 A resolution. EMBO J. 19:6311-6316.→Abstract
K. Murata, K. Mitsuoka, T. Hirai, T. Walz, P. Agre, J. B. Heymann, A. Engel, Y. Fujiyoshi. (2000) Structural determinants of water permeation through aquaporin-1. Nature 407:599-605.→Abstract
B. L. de Groot, J. B. Heymann, A. Engel, K. Mitsuoka, Y. Fujiyoshi, H. Grubmuller. (2000) The fold of human aquaporin 1. J. Mol. Biol. 300:987-994.→Abstract
B. Wu, L. Hammar, L. Xing, S. Markarian, J. Yan, K. Iwasaki, Y. Fujiyoshi, T. Omura, H. Cheng. (2000) Phytoreovirus T = 1 core plays critical roles in organizing the outer capsid of T = 13 quasi-equivalence. Virology 271:18-25.→Full-text
A. Engel, Y. Fujiyoshi, P. Agre. (2000) The importance of aquaporin water channel protein structures. EMBO J. 19:800-806.→Abstract
F. Imamura, I. Arimoto, Y. Fujiyoshi, T. Doi. (2000) W276 mutation in the endothelin receptor subtype B impairs Gq coupling but not Gi or Go coupling. Biochemistry. 39:686-692.→Abstract

1977-1999

1991-1999
T. Doi, H. Sugimoto, I. Arimoto, Y. Hiroaki, Y. Fujiyoshi. (1999) Interactions of endothelin receptor subtypes A and B with Gi, Go, and Gq in reconstituted phospholipid vesicles. Biochemistry. 38:3090-3099.→Abstract
Y. Fujiyoshi. (1999) Molecular structure of proton pump revealed with electron crystallography. FASEB. J. 13. Suppl. 2:S191-S194.→PubMed
T. Hirai, K. Murata, K. Mitsuoka, Y. Kimura, Y. Fujiyoshi. (1999) Trehalose embedding technique for high-resolution electron crystallography: application to structural study on bacteriorhodopsin. J. Electron. Microsc. (Tokyo.) 48:653-658.→Abstract
Y. Hiroaki, K. Mitsuoka, Y. Cheng, H. Hiroaki, Y. Fujiyoshi. (1999) Influence of various nucleotides on the in situ crystallization of Ca2+-ATPase. Biochim. Biophys. Acta. 1415:361-368.→Full-text
K. Mitsuoka, T. Hirai, K. Murata, A. Miyazawa, A. Kidera, Y. Kimura, Y. Fujiyoshi. (1999) The structure of bacteriorhodopsin at 3.0 A resolution based on electron crystallography: implication of the charge distribution. J. Mol. Biol. 286:861-882.→Abstract
K. Mitsuoka, K. Murata, T. Walz, T. Hirai, P. Agre, J. B. Heymann, A. Engel, Y. Fujiyoshi. (1999) The structure of aquaporin-1 at 4.5- A resolution reveals short alpha-helices in the center of the monomer. J. Struct. Biol. 128:34-43.→Abstract
A. Miyazawa, Y. Fujiyoshi, M. Stowell, N. Unwin. (1999) Nicotinic acetylcholine receptor at 4.6 A resolution: transverse tunnels in the channel wall. J. Mol. Biol. 288:765-786.→Abstract
I. Schmidt-Krey, K. Murata, T. Hirai, K. Mitsuoka, Y. Cheng, R. Morgenstern, Y. Fujiyoshi, H. Hebert. (1999) The projection structure of the membrane protein microsomal glutathione transferase at 3 A resolution as determined from two-dimensional hexagonal crystals. J. Mol. Biol. 288:243-253.→Abstract
Y. Fujiyoshi. (1998) The structural study of membrane proteins by electron crystallography. Adv. Biophys. 35:25-80.→Abstract
I. Ikeda-Yamasaki, T. Odahara, K. Mitsuoka, Y. Fujiyoshi, K. Murata. (1998) Projection map of the reaction center-light harvesting 1 complex from Rhodopseudomonas viridis at 10 A resolution. FEBS. Lett. 425:505-508.→Abstract
Y. Mimori-Kiyosue, I. Yamashita, Y. Fujiyoshi, S. Yamaguchi, K. Namba. (1998) Role of the outermost subdomain of Salmonella flagellin in the filament structure revealed by electron cryomicroscopy. J. Mol. Biol. 284:521-530.→Abstract
T. Ohtaki, K. Ogi, Y. Masuda, K. Mitsuoka, Y. Fujiyoshi, C. Kitada, H. Sawada, H. Onda, M. Fujino. (1998) Expression, purification, and reconstitution of receptor for pituitary adenylate cyclase-activating polypeptide. large-scale purification of a functionally active G protein-coupled receptor produced in Sf9 insect cells. J. Biol. Chem. 273:15464-15473.→Abstract
T. Doi, Y. Hiroaki, I. Arimoto, Y. Fujiyoshi, T. Okamoto, M. Satoh, Y. Furuichi. (1997) Characterization of human endothelin B receptor and mutant receptors expressed in insect cells. Eur. J. Biochem. 248:139-148.→Abstract
H. Hebert, I. Schmidt-Krey, R. Morgenstern, K. Murata, T. Hirai, K. Mitsuoka, Y. Fujiyoshi. (1997) The 3.0 A projection structure of microsomal glutathione transferase as determined by electron crystallography of p 21212 two-dimensional crystals. J. Mol. Biol. 271:751-758.→Abstract
Y. Kimura, D. G. Vassylyev, A. Miyazawa, A. Kidera, M. Matsushima, K. Mitsuoka, K. Murata, T. Hirai, Y. Fujiyoshi. (1997) Surface of bacteriorhodopsin revealed by high-resolution electron crystallography. Nature. 389:206-211.→Abstract
T. Walz, T. Hirai, K. Murata, J. B. Heymann, K. Mitsuoka, Y. Fujiyoshi, B. L. Smith, P. Agre, A. Engel. (1997) The three-dimensional structure of aquaporin-1. Nature. 387:624-627.→Abstract
Y. Mimori-Kiyosue, F. Vonderviszt, I. Yamashita, Y. Fujiyoshi, K. Namba. (1996) Direct interaction of flagellin termini essential for polymorphic ability of flagellar filament. Proc. Natl. Acad. Sci. U. S. A. 93:15108-15113.→Abstract
K. Aoyama, K. Ogawa, Y. Kimura, Y. Fujiyoshi. (1995) A method for 2D crystallization of soluble proteins at liquid-liquid interface. Ultramicroscopy. 57:345-354.→Abstract
Y. Mimori, I. Yamashita, K. Murata, Y. Fujiyoshi, K. Yonekura, C. Toyoshima, K. Namba. (1995) The structure of the R-type straight flagellar filament of Salmonella at 9 A resolution by electron cryomicroscopy. J. Mol. Biol. 249:69-87.→Abstract
Y. Fujiyoshi, N. P. Kume, K. Sakata, S. B. Sato. (1994) Fine structure of influenza A virus observed by electron cryo-microscopy. EMBO. J. 13:318-326.→Abstract
W. Kuhlbrandt, D. N. Wang, Y. Fujiyoshi. (1994) Atomic model of plant light-harvesting complex by electron crystallography. Nature. 367:614-621.→Abstract
Y. Tahara, Y. Fujiyoshi. (1994) A new method to measure bilayer thickness: cryo-electron microscopy of frozen hydrated liposomes and image simulation. Micron. 25:141-149.→Abstract
Y. Tahara, S. Ohnishi, Y. Fujiyoshi, Y. Kimura, Y. Hayashi. (1993) A pH induced two-dimensional crystal of membrane-bound Na+,K+-ATPase of dog kidney. FEBS. Lett. 320:17-22.→Abstract
Y. Fujiyoshi, T. Mizusaki, K. Morikawa, H. Yamagishi, Y. Aoki, H. Kihara, Y. Harada. (1991) Development of a Superfluid Helium Stage for High-Resolution Electron Microscopy. Ultramicroscopy. 38:241-251.→Abstract
H. Fan, S. Xiang, F. Li, Q. Pan, N. Uyeda and Y. Fujiyoshi. (1991) Image Resolution Enhancement by Combining Information from Electron Diffraction Pattern and Micrograph. Ultramicroscopy. 36:361-365.→Abstract
1981-1990
Y. Liu, S. Xiang, H. Fan, D. Tang, F. Li, Q. Pan, N. Uyeda and Y. Fujiyoshi. (1990) Image Deconvolution of a Single High-Resolution Electron Micrograph. Acta Crystallographica. A46:459-463.→Abstract
Y. Fujiyoshi. (1989) High Resolution Cryo-Electron Microscopy for Biological Macromolecules. J. Electron Microsc. 38:97-101.→Abstract
M. Ashida, Y. Uyeda, H. Yanagi, N. Uyeda, Y. Fujiyoshi and J. R. Fryer. (1988) Formation and Structure of a 2,3,9,10,16,17,23,24-Octacyanophalocyanine- Potassium Complex in Thin Film. Acta Crystallographica. B44:146-151.→Abstract
N. Uyeda, T. Takenaka, K. Aoyama, M. Matsumoto and Y. Fujiyoshi. (1987) Holes in a Stearic Acid Monolayer Observed by Dark-Field Electron Microscopy. Nature. 327:319-321.→Abstract
K. Kajiwara, N. Donkai, Y. Fujiyoshi and H. Inagaki. (1986) Lyotropic Mesophase of Imogolite, 2 Microscopic Observation of Imogolite Mesophase. Makromol. Chem. 187:2895-2907.→Abstract
J. Sugiyama, H. Harada, Y. Fujiyoshi and N. Uyeda. (1985) Lattice Imagies from Ultrathin Sections of Cellulose Microfibrils in the Cell Wall of Valonia Macrophysa Kütz. Planta. 166:161-168.→Abstract
K. Morikawa, Y. Fujiyoshi, K. Ishizuka, M. Kawakami and S. Takemura. (1985) Electron Microscopic Study of 5S rRNA Crystals from Thermus thermophilus HB8. Journal of Microscopy. 142:247-258.→Abstract
N. Uyeda, T. Kobayashi, K. Ishizuka, Y. Fujiyoshi, H.Inockuchi and G. Saito. (1985) Direct Molecular Imaging of Low Dimensional Solids by High Resolution Electron Microscopy. Mol. Cryst. Liq. Cryst. 125:103-112.→Abstract
J. Sugiyama, H. Harada, Y. Fujiyoshi and N. Uyeda. (1985) Observations of Cellulose Microfibrils in Valonia macrophysa by High resolution Electron Microscopy. Mokuzai Gakkaishi. 31:61-67.
E. J. Kirkland, B. M. Siegel, N. Uyeda and Y. Fujiyoshi. (1985) Improved High Resolution Image Processing of Bright Field Electron Micrographs: Part II Experiment. Ultramicroscopy. 17:87-104.→Abstract
K. Morikawa, Y. Fujiyoshi, K. Ishizuka, M. Kwakami and S. Takemura. (1984) Various Types of 5S rRNA Crystals as Studied by X-ray Diffraction and Electron Microscopy. Nucleic Acids Res. 15:143-146.→Abstract
N. Uyeda, Y. Fujiyoshi and K. Ishizuka. (1984) High Resolution Imaging and Interpretation of Regular and Irregular Structures in n-Niobium Pentoxide Crystal. Ultramicroscopy. 15:139-150.→Abstract
Y. Fujiyoshi, N. Uyeda, K. Morikawa and H. Yamagishi. (1984) Electron Microscopy of tRNA Crystals II: 4Å Resolution Diffraction Pattern and Substantial Stability to Radiation Damage. J. Mol. Biol. 172:347-354.→Abstract
Y. Fujiyoshi, K. Morikawa, N. Uyeda, H. Ozeki and H. Yamagishi. (1984) Electron Microscopy of tRNA Crystals I: Thin Crystals Negatively Stained with Uranyl Acetate. Ultramicroscopy. 12:201-212.→Abstract
J. Sugiyama, H. Harada, Y. Fujiyoshi and N. Uyeda. (1984) High Resolution Observation of Cellulose Microfibrils. Mokuzai Gakkaishi. 30:98-99.
T. Kobayashi, Y. Fujiyoshi and N. Uyeda. (1983) High-Resolution Electron Microscopy of Structural Defects in Organic Crystals. J. Crystal Growth. 65:511-517.→Abstract
F. Takeda, M. Matsumoto, T. Takenaka, Y. Fujiyoshi and N. Uyeda. (1983) Surface Pressure Dependence on Monolayer Structure of Poly-e-Benzyloxycarbonyl-L-Lysine. J. Colloid and Interface Science. 91:267-271.→Abstract
E. J. Kirkland, B. M. Siegel, N. Uyeda and Y. Fujiyoshi. (1982) Nonlinear High Resolution Image Processing of Conventional Transmission Electron Micrograph, II Experiment. Ultramicroscopy. 9:65-74.→Abstract
T. Kobayashi, Y. Fujiyoshi and N. Uyeda. (1982) The Observation of Molecular Orientation in Crystal Defects and Growth Mechanism of Thin Phthalocyanine Films. Acta Cryst. A38:356-362.→Abstract
M. Mabuchi, T. Takenaka, Y. Fujiyoshi and N. Uyeda. (1982) Surface Enhanced Raman Scattering of Citrate Ions Adsorbed on Gold Sol Particles. Surface Science. 119:150-158.→Abstract
O. Ohara, S. Takahashi, T. Ooi and Y. Fujiyoshi. (1982) Cross-Linking Study on Skeletal Muscle Actin: Properties of Suberimidate-Treated Actin. J. Biochem. 91: 1999-2012.→Abstract
Y. Fujiyoshi, H. Yamagishi, T. Kunisada, H. Sugisaki, T. Kobayashi and N. Uyeda. (1982) Visualization of the DNA Thread Packing within Bacteriophage T4 Heads. J. Ultrastructure Research. 79:235-240. →Abstract
F. Takeda, M. Matsumoto, T. Takenaka and Y. Fujiyoshi. (1981) Studies of Poly-g-Methyl-L-Glutamate Monolayers by Infrared ATR and Transmission Spectroscopy and Electron Microscopy. J. Colloid and Interface Science. 84:220-227. →Abstract
T. Kobayashi, Y. Fujiyoshi, K. Ishizuka and N. Uyeda. (1981) High Resolution TEM Images of Zinc-Phthalocyanine Polymorphs in Thin Films. Acta Cryst. A37: 692-697.→Abstract
O. Ohara, S. Takahashi, T. Ooi and Y. Fujiyoshi. (1981) Fixation of Skeletal Muscle Actin in f-State by Chemical Cross-Linking with Bis-Imidoesters. Biochem. Biophys. Research Communications. 100:988-994.→Abstract
Y. Fujiyoshi and N. Uyeda. (1981) Direct Imaging of Double-Strand DNA Molecule. Ultramicroscopy. 7:189-192.→First page preview
1977-1980
E. J. Kirkland, B. M. Siegel, N. Uyeda and Y. Fujiyoshi. (1980) Digital Reconstruction of Bright Field Phase Contrast Images from High Resolution Electron Micrographs. Ultramicroscopy. 5:479-503.→Abstract
Y. Fujiyoshi, T. Kobayashi, K. Ishizuka, N. Uyeda, Y. Ishida and Y. Harada. (1980) A New Method for Optimal-Resolution Electron Microscopy of Radiation-Sensitive Specimens. Ultramicroscopy. 5:459-468.→Abstract
N. Uyeda, T. Kobayashi, K. Ishizuka and Y. Fujiyoshi. (1980) Crystal Structure of Ag-TCNQ. Nature. 285:95-97.→Abstract
N. Uyeda, T. Kobayashi, K. Ishizuka and Y. Fujiyoshi. (1978/79) High Voltage Electron Microscopy for Image Discrimination of Constituent Atoms in Crystals and Molecules. Chemica Scripta. 14:47-61.
Y. Fujiyoshi, and N. Uyeda. (1978) The Alumina Supermicrogrid for High Resolution Electron Microscopy. J. Electron microsc. 27:75-82.→Abstract
Y. Fujiyoshi, K. Ishizuka and N. Uyeda. (1977) Structure Analysis of a New Type of Vanadium Oxide by High Resolution Electron Microscopy. J. Electron microsc. 26:47-49.→First page preview