pDEST Series
Promoter/3' Cassette
pDEST vectors provide a promoter and a 3' cassette for constructing a variety of Gateway Expression vectors.
pDEST vectors provide a promoter and a 3' cassette for constructing a variety of Gateway Expression vectors.
For Worms
The sequence information is available in C. elegans Promoter/Marker Database supported by National BioResource Project or upon request to KUROYANAGI Hidehito.
| pDEST-PL | Promoterless |
| pDEST-aex-3p | Pan-neuronal |
| pDEST-che-2p | Amphid sensory neurons |
| pDEST-dpy-7p | Hypodermal cells |
| pDEST-eat-4p | Glutamatergic neurons |
| pDEST-eft-3p | Ubiquitous |
| pDEST-elt-2p | Intestine |
| pDEST-F25B3.3p | Pan-neuronal |
| pDEST-ges-1p | Intestine |
| pDEST-gon-2p | Intestine |
| pDEST-gst-42p | Intestine |
| pDEST-gtl-1p | Intestine |
| pDEST-hsp16-2p | Heat shock inducible |
| pDEST-hsp16-41p | Heat shock inducible |
| pDEST-mec-7p | Touch receptor neurons |
| pDEST-myo-2p | Pharyngeal muscles |
| pDEST-myo-3p | Body wall muscles, vulval muscles |
| pDEST-sdf-9p | XXXL/R |
| pDEST-unc-4p | Subset of motor neurons (DA, VA, VC, SAB) |
For Bacteria
For constructing cold-shock-inducible expression vectors.
The sequence information is available upon request to KUROYANAGI Hidehito.
The sequence information is available upon request to KUROYANAGI Hidehito.
| pDEST-Cold II | N-terminal His tag |
| pDEST-Cold-FLAG | N-terminal FLAG tag |
| pDEST-Cold-GST | N-terminal His-GST tag |
| pDEST-Cold-ZZ | N-terminal His-ZZ tag |
For Cultured Cells
The sequence information is available upon request to KUROYANAGI Hidehito.
| pDEST-cDNA3 | CMV promoter |
| pDEST-cDNA3-FLAG | CMV promoter N-terminal FLAG tag |
| pDEST-ME18S-3HA | SRalpha promoter N-terminal 3xHA tag |