As of December 3, 2018.

Eichi Watabe, Shoichiro Ono and Hidehito Kuroyanagi.
Alternative splicing of the Caenorhabditis elegans lev-11 tropomyosin gene is regulated in a tissue-specific manner.
Cytoskeleton. 75: 427-436, 2018.
PMID: 30155988
Cover Image: Volume 75, Issue 10

Hiroaki Iwasa, Aradhan Sarkar, Takanobu Shimizu, Takeru Sawada, Shakhawoat Hossain, Xiaoyin Xu, Junichi Maruyama, Kyoko Arimoto‐Matsuzaki, Kanchanamala Withanage, Kentaro Nakagawa, Hidetake Kurihara, Hidehito Kuroyanagi, Yutaka Hata.
UNC119 is a binding partner of a tumor suppressor RASSF6 and induces apoptosis and cell cycle arrest via MDM2 and p53.
Cancer Science. 109: 2767-2780, 2018.
PMID:29931788

Rie Murayama, Mariko Kimura-Asami, Marina Togo-Ohno, Yumiko Yamasaki-Kato, Taeko K. Naruse, Takeshi Yamamoto, Takeharu Hayashi, Tomohiko Ai, Katherine G. Spoonamore, Richard J. Kovacs, Matteo Vatta, Mai Iizuka, Masumi Saito, Shotaro Wani, Yuichi Hiraoka, Akinori Kimura and Hidehito Kuroyanagi.
Phosphorylation of the RSRSP stretch is critical for splicing regulation by RNA-Binding Motif Protein 20 (RBM20) through nuclear localization.
Scientific Reports. 8: 8970, 2018.
PMID:29895960
Press Release: Of Hearts and Giants: Moving a Cardiac Regulatory Protein to the Right Place
EurekAlert!: Of hearts and giants: Moving a cardiac regulatory protein to the right place
Science Daily: Of hearts and giants: Moving a cardiac regulatory protein to the right place
Technology Networks: Of Hearts and Giants: Biggest Protein Helps Study Cardiac Disease
MedicalXpress: Moving a cardiac regulatory protein to the right place

Barnes DE, Watabe E, Ono K, Kwak E, Kuroyanagi H, Ono S.
Tropomyosin isoforms differentially affect muscle contractility in the head and body regions in the nematode Caenorhabditis elegans.
Molecular Biology of the Cell. 29: 1075-1088, 2018.
PMID:29496965

Masahiro Tomioka, Yasuki Naito, Hidehito Kuroyanagi & Yuichi Iino.
Splicing factors control C. elegans behavioural learning in a single neuron by producing DAF-2c receptor.
Nature Communications. doi:10.1038/ncomms11645, 2016.
PMID:27198602
UTokyo Research: Production of a molecule for memory of salt concentration
F1000Prime: Recommended by Yishi Jin
Neuroscience News: Production of a Molecule for Memory of Salt Concentration

Takei S, Togo-Ohno M, Suzuki Y, Kuroyanagi H.
Evolutionarily conserved autoregulation of alternative pre-mRNA splicing by ribosomal protein L10a.
Nucleic Acids Research. 44: 5585-5596, 2016.
PMID:26961311

Kuwasako K, Takahashi M, Unzai S, Tsuda K, Yoshikawa S, He F, Kobayashi N, Güntert P, Shirouzu M, Ito T, Tanaka A, Yokoyama S, Hagiwara M, Kuroyanagi H, Muto Y.
RBFOX and SUP-12 sandwich a G base to cooperatively regulate tissue-specific splicing.
Nature Structural & Molecular Biology. 21, 778–786, 2014.
PMID:25132178
MedicalXpress: How pairs of proteins collaborate to splice RNAs into cell-specific products

Kuroyanagi H, Takei S, Suzuki Y.
Comprehensive analysis of mutually exclusive alternative splicing in C. elegans.
Worm. 3: e28459, 2014.
PMID:25254147

Kuroyanagi H, Watanabe Y, Hagiwara M.
CELF family RNA-binding protein UNC-75 regulates two sets of mutually exclusive exons of the unc-32 gene in neuron-specific manners in Caenorhabditis elegans.
PLoS Genetics. 9: e1003337, 2013.
PMID:23468662

Kuroyanagi H, Watanabe Y, Suzuki Y, Hagiwara M.
Position-dependent and neuron-specific splicing regulation by the CELF family RNA-binding protein UNC-75 in Caenorhabditis elegans.
Nucleic Acids Research. 41: 4015-4025, 2013.
PMID:23416545

Iwasa H, Maimaiti S, Kuroyanagi H, Kawano S, Inami T, Ikeda M, Nakagawa K, Hata Y.
Yes-associated protein homolog, YAP-1, is involved in the thermotolerance and aging in the nematode Caenorhabditis elegans.
Experimental Cell Research. 319: 931–945, 2013.
PMID:23396260

Iwasa H, Kuroyanagi H, Maimaiti S, Ikeda M, Nakagawa K, Hata Y.
Characterization of RSF-1, the Caenorhabditis elegans homolog of the Ras-association domain family protein 1.
Experimental Cell Research. 319: 1–11, 2013.
PMID:23103556

Ohno G, Ono K, Togo M, Watanabe Y, Ono S, Hagiwara M, Kuroyanagi H.
Muscle-Specific Splicing Factors ASD-2 and SUP-12 Cooperatively Switch Alternative Pre-mRNA Processing Patterns of the ADF/Cofilin Gene in Caenorhabditis elegans.
PLoS Genetics. 8: e1002991, 2012.
PMID:23071450

Kuroyanagi H, Ohno G, Sakane H, Maruoka H, Hagiwara M.
Visualization and genetic analysis of alternative splicing regulation in vivo using fluorescence reporters in transgenic Caenorhabditis elegans.
Nature Protocols. 5: 1495-1517, 2010.
PMID:20725066

Ohno G, Hagiwara M, Kuroyanagi H.
STAR family RNA-binding protein ASD-2 regulates developmental switching of mutually exclusive alternative splicing in vivo.
Genes & Development. 22: 360-374, 2008.
PMID:18230701
F1000Prime: Recommended by Thomas Blumenthal

Kuroyanagi H, Ohno G, Mitani S, Hagiwara M.
The Fox-1 family and SUP-12 coordinately regulate tissue-specific alternative splicing in vivo.
Molecular and Cellular Biology. 27: 8612-8621, 2007.
PMID:17923701

Gengyo-Ando K, Kuroyanagi H, Kobayashi T, Murate M, Fujimoto K, Okabe S, Mitani S.
The SM protein VPS-45 is required for RAB-5-dependent endocytic transport in Caenorhabditis elegans.
EMBO reports. 8: 152-157, 2007.
PMID:17235359

Kuroyanagi H, Kobayashi T, Mitani S, Hagiwara M.
Transgenic alternative-splicing reporters reveal tissue-specific expression profiles and regulation mechanisms in vivo.
Nature Methods. 3: 909-915, 2006.
PMID:17060915

Takeshi Watanabe, Akinori Kimura, Hidehito KUROYANAGI.
Alternative splicing regulator RBM20 and cardiomyopathy.
Frontiers in Molecular Biosciences, section RNA, 5: 105, 2018.
PMID:30547036

Wani, S. and Kuroyanagi, H.
An emerging model organism Caenorhabditis elegans for alternative pre-mRNA processing in vivo.
WIREs RNA, e1428. doi: 10.1002/wrna.1428, 2017.
PMID:28703462
Advanced Science News: Caenorhabditis elegans: An Emerging Model Organism

Hidehito Kuroyanagi.
Switch-like regulation of tissue-specific alternative pre-mRNA processing patterns revealed by customized fluorescence reporters.
Worm. 2: e23834, 2013.
PMID:24778931

Hidehito Kuroyanagi, Akihide Takeuchi, Takayuki Nojima and Masatoshi Hagiwara.
Chapter 28: Single cell detection of splicing events with fluorescent splicing reporters.
In "Alternative pre-mRNA Splicing: Theory and Protocols" (Stamm, S./ Smith, C./ Lührmann, R. Eds, Wiley-VCH). 2012.

Kuroyanagi H.
[New approaches to decipher pre-mRNA splicing codes]. [Article in Japanese]
Seikagaku. 82: 402-411, 2010.
PMID:20560432.

Kuroyanagi H.
[Visualization of alternative splicing events in vivo]. [Article in Japanese]
Tanpakushitsu Kakusan Koso. 54: 2044-2048, 2009.
PMID:21089615.

Kuroyanagi H.
Fox-1 family of RNA-binding proteins.
Cell Mol Life Sci. 66: 3895-3907, 2009.
PMID:19688295.

Hidehito Kuroyanagi, Kensuke Ihara, Marina Ohno-Togo, Akinori Kimura and Tetsuo Sasano.
Phosphorylation of RBM20 on the RSRSP stretch, a hot spot of mutations in dilated cardiomyopathy, is critical for splicing regulation in the mouse heart.
The 2nd Joint Australia-Japan/Japan-Australia joint RNA meeting 2018, Sapporo, Hokkaido, November, 2018.

Hidehito Kuroyanagi
"Non-Coding mRNAs" from Protein-Coding Genes through Alternative Pre-mRNA Splicing.
19th Tokyo RNA Club, University of Tokyo, Bunkyo-ku, Tokyo. April, 2016.

Hidehito Kuroyanagi
RBFOX and SUP-12 cooperatively regulate muscle-specific alternative splicing to determine ligand-binding specificity of FGF receptors in C. elegans.
RIKEN Symposium "Noncoding RNA regulation", RIKEN, Wako, Saitama, October, 2014.

Hidehito Kuroyanagi
Cooperative regulation of tissue-specific alternative splicing by multiple splicing factors determines ligand-binding specificity of FGF receptors.
The 9th International Symposium of the Institute Network, Osaka University, Suita, Osaka, June, 2014.

Hidehito Kuroyanagi, Marina Togo, Yohei Watanabe, Motoki Hoshina, Yutaka Suzuki and Masatoshi Hagiwara.
Multi-domain protein LST-3 regulates pre-mRNA transcription and alternative splicing in C. elegans.
CSHL Meeting on Eukaryotic mRNA Processing, Cold Spring Harbor, NY, USA, August, 2013．

Hidehito Kuroyanagi.
Regulation of tissue-specific alternative splicing by the RBFOX family.
Harvard FAS Center for Systems Biology, Cambridge, MA, USA, August, 2013．

Hidehito Kuroyanagi.
Genetic analyses of alternative splicing regulation in C. elegans.
University of California, Los Angeles, CA, USA, June 2013．

Genta Ohno, Kanako Ono, Marina Togo, Yohei Watanabe, Shoichiro Ono, Masatoshi Hagiwara, Hidehito Kuroyanagi
Muscle-Specific Splicing Factors ASD-2 and SUP-12 Cooperatively Switch Alternative Pre-mRNA Processing Patterns of the ADF/Cofilin Gene in C. elegans.
Cold Spring Harbor Asia conferences on RNA Biology, Suzhou Dushu Lake Conference Center, Suzhou, China. Oct, 2012.

Hidehito Kuroyanagi
Deciphering splicing codes by visualizing alternative splicing patterns in vivo.
Institute of Molecular and Cellular Biology, National Taiwan University, Taipei, Taiwan. Nov, 2011.

Hidehito Kuroyanagi
Regulation of tissue-Regulation of tissue-specific alternative splicing in C. elegans.
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan. Nov, 2011.

Genta Ohno, Kanako Ono, Yohei Watanabe, Marina Togo, Shoichiro Ono, Masatoshi Hagiwara, Hidehito Kuroyanagi
ASD-2 and SUP-12 cooperatively regulate muscle-specific alternative splicing of the ADF/cofilin gene in C. elegans.
NTU-JST Joint Meeting on RNA and Biofunctions – Asia Studies, Taipei, Taiwan. Nov, 2011.

Hidehito KUROYANAGI.
Regulation of Tissue-Specific Alternative Splicing in C. elegans.
The 19th CDB Meeting "RNA Sciences in Cell and Developmental Biology" , CDB-RIKEN, Kobe. May, 2010.

Hidehito KUROYANAGI.
Tissue-specific alternative splicing regulation in C. elegans.
GENE REGULATION PROGRAMME SEMINAR, ICREA and Centre de Regulacio Genomica, Barcelona, Spain. December, 2009.

Hidehito KUROYANAGI.
Tissue-specific alternative splicing regulation by Fox-1 family in C. elegans.
RNA Club, Institut Europeen de Chimie et Biologie (Bordeaux), France. December, 2009.

Hidehito KUROYANAGI.
A transgenic reporter system reveals expression profiles and regulation mechanisms of alternative splicing in vivo.
8th Surugadai International Symposium, TMDU, Tokyo. September, 2009.

Hidehito KUROYANAGI.
Tissue-specific alternative splicing regulation by Fox-1 family in C. elegans.
Seminar, Howard Hughes Medical Institute/UCLA, CA, USA. June 2009.

Hidehito KUROYANAGI.
Alternative splicing reporters offer a path to splicing codes.
National BioResource Project C. elegans Symposium "Nematode: an ideal model organism for functional genomics". Tokyo Women's Medical University, Tokyo. November, 2008.

Hidehito KUROYANAGI.
A transgenic reporter system reveals expression profiles and regulation mechanisms of alternative splicing in vivo.
UK-Japan Frontiers of Science Symposium 2008, Kanagawa. October, 2008.

Hidehito KUROYANAGI.
Visualization of alternative splicing patterns in vivo reveals regulation mechanisms.
RNA2008 "Ambitious Young Scientists Looking toward the Next Decade", Sapporo. July, 2008.

Hidehito KUROYANAGI.
Visualization of alternative splicing patterns in vivo reveals regulation mechanisms.
Department of Genetics, Case Western Reserve University, Cleveland, OH, USA. July, 2008.

Hidehito KUROYANAGI.
Alternative Splicing Reporter Worms – Visualization of Spatiotemporal Regulation in vivo.
University of California Los Angeles/Howard Hughes Medical Institute, Los Angeles, CA, USA. July, 2007.

Hidehito Kuroyanagi.
Transgenic alternative-splicing reporters reveal tissue-specific expression profiles and regulation mechanisms in vivo.
RIKEN Center for Developmental Biology, Kobe. November, 2006.

Kuroyanagi, H., for Hagiwara, M.
Visualization of tissue-specific alternative splicing and cloning of the regulatory proteins in C. elegans.
Gordon Research Conference, The Biology Of Post-Transcriptional Gene Regulation, Oxford, UK. August, 2006.

Our researches are supported by the following funds.

Ministry of Education,Culture,Sports,Science & Technology (MEXT) in Japan
Grants-in-Aid for Scientific Research

Takeda Science Foundation

Japan Science and Technology Agency (JST)
PRESTO

Japan Society for the Promotion of Science (JSPS)
Joint Research Projects
Grants-in-Aid for Scientific Research