Observation and analysis of organella and intracellular structures with superresolution microscopy.

Research abstract

Optical super resolution imaging is a powerful tool for observation and analysis on the organella zone. Optical microscopy has been used to observe samples of mm order. But it has been  difficult to observe detail of intracellular organella, In recent years, super resolution is developed to overcome the resolution limit of conventional optical microscopy. Intracellular ollganella ranging from 30nm to 150nm are supposed to be suitable samples for observation with super resolution. We will develop and/or introduce new methods and techniques of conventional and/or super resolution microscopy which are useful for observation and analysis of organella zones. Technical assistances of super resolution microscopy for other researchers are also included as a part of research projects.

Original papers

1. Ohzono T.. Katoh K., Terentjev E.M. (2021)
   Microscopy of diffuse nematic-isotropic transition in main-chain nematic liquid crystal elastomers. Macromolecules (in press)

2. Ohzono T., Katoh K., Minamikawa H., Saed M. O., Terentjev E.M. (2021)
   Internal constraints and arrested relaxation in main-chain nematic elastomers
   Nat Commun 12, 787 (2021). https://doi.org/10.1038/s41467-021-21036-3

3. Higaki T., Akita K., Katoh K (2020)
   Coefficient of variation as an image-intensity metric for cytoskeleton bundling
   Sci. Rep. 10, 22187. https://doi.org/10.1038/s41598-020-79136-x

4. Yamaguchi H., Honda S., Torii S., Shimizu K., Katoh K., Miyake K., Miyake N., Fujikake N.,
   Sakurai H.T., Arakawa S., Shimizu S.(2020)
   Wipi3 is essential for alternative autophagy and its loss causes neurodegeneration. Nat Commun 11, 5311. https://doi.org/10.1038/s41467-020-18892

5. Ueno Y., Matsuda K., Katoh K., Kuzuya A., Kakugo A., Konagaya A. (2020)  Modeling a Microtubule Filaments Mesh Structure from Confocal Microscopy Imaging. Micromachines 11 (9), 844 https://doi.org/10.3390/mi11090844

6. Ishida K., Goto S., Ishimura M., Amanuma M., Hara Y., Suzuki R., Katoh K., Morita E. (2019)
   Functional Correlation between Subcellular Localizations of Japanese Encephalitis Virus Capsid Protein and Virus Production.
   J Virol 93:e00612-19. https://doi.org/10.1128/JVI.00612-19

7. Morita, M, Ota, Y, Katoh, K, Noda, N (2019)  Bacterial Cell Culture at the Single-cell Level Inside Giant Vesicles.
   JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (146):10.3791/59555

8. Tanaka, M., Fujii, Y., Hirano, K., Higaki, T., Nagasaki, A., Ishikawa, R., Okajima, T., Katoh, K.(2019) Fascin in lamellipodia contributes to cell elasticity by controlling the orientation of filamentous actin. Genes to Cells. 24, p202-213, https://doi.org/10.1111/gtc.12671
9. Takata, H., Madung, M., Katoh, K., Fukui, K.(2019) Cdk1-dependent phosphorylation of KIF4A at S1186 triggers lateral chromosome compaction during early mitosis. PLoS One 13(12): e0209614 doi.org/10.1371/journal.pone.0209614
10. Morita, M., Katoh,K., Noda, N.(2018) Direct Observation of Bacterial Growth in Giant Unilamellar Vesicles: A Novel Tool for Bacterial Cultures. ChemistryOpen https://doi.org/10.1002/open.201800126
11. Kijima, S. Staiger, C., Katoh, K., Nagasaki, A., Ito, K., and Uyeda, T. (2018)　 Arabidopsis vegetative actin isoforms, AtACT2 and AtACT7, generate distinct filament arrays in living plant cells. Sci Rep., 8:4381 | DOI:10.1038/s41598-018-22707-w.
12. Ito, N., Katoh, K., Kushige, H., Saito, Y., Umemoto, T., Matsuzaki, Y., Kiyonari, H., Kobayashi, D., Soga, M., Era, T., Araki, N., Furuta, Y., Suda, T., Kida, Y., and Ohta K. (2018) Ribosome Incorporation into Somatic Cells Promotes Lineage Transdifferentiation towards Multipotency. Sci Rep. 8, 1634 | DOI:10.1038/s41598-018-20057-1
13. Ohzono, T., Katoh, K., Wang, C., Fukazawa, A., Yamaguchi, S., and Fukuda, J. (2017) Uncovering different states of topological defects in schlieren textures of a nematic liquid crystal. Sci. Rep. 7, 16814 | DOI:10.1038/s41598-017-16967-1
14. Nozumi, M., Nakatsu, F., Katoh, K., and Igarashi, M. (2017) The Coordinated Vesicle and Actin Bundle Movement in Nerve Growth Revealed by Superresolution Microscopy. Cell Rep. 18 2203–2216
15. Ohzono, T., Katoh, K., and Fukuda, J. (2016) Fluorescence microscopy reveals molecular localisation at line defects in nematic liquid crystals. Sci. Rep. 6: 36477 | DOI: 10.1038/srep36477
16. Shibata, T., Yamashita, S., Hirusaki, K., Katoh K., Ohta, Y. (2015) Isolation of mitochondria by gentle cell membrane disruption, and their subsequent characterization. Biochem. Biophys. Res. Commun. 463 563-568
17. Sugiura, S., Nishimura, S., Yasuda, S., Hosoya, Y., and Katoh, K. (2006) Caron fiber technique for the investigation of single-cell mechanics in intact cardiac myocytes. Nat. Protoc. 1, 1453-1457