Application of genome-wide siRNA screen technology to cellular stress response

Research abstract

We have established and been preforming a genome-wide siRNA screen system to explore components of signal transduction involved in a specific stress response. In the system, we employ an arrayed-format siRNA library in 384 well plates (over 18,000 target genes) and use a high-content image analyzer to evaluate cell phenotype, which enables us to quantify the spatial information in the cell including organelles. In eukaryotic cells, several organelles function as field of stress sensing and response. We will apply our genome-wide siRNA screen system in the project of organelle zone to clarify the molecular mechanisms of signal transduction involving organelle formation/deformation.

Original papers

1. Watanabe, K., Umeda, T., Niwa, K., Naguro, I. and Ichijo, H. (2018) A PP6-ASK3 module coordinates the bidirectional cell volume regulation under osmotic stress. Cell Rep. 13, 2809-2817. 
2. Hattori, K., Ishikawa, H., Sakauchi, C., Takayanagi, S., Naguro, I. and Ichijo, H. (2017) Cold stress-induced ferroptosis involves the ASK1-p38 pathway. EMBO Rep.18, 2067-2078. 

総説

1. Zhou, X., Naguro, I., Ichijo, H., and Watanabe, K. (2016) Mitogen-activated protein kinases as key players in osmotic stress signaling. Biochim. Biophys. Acta., 1860, 2037-2052