Identification and functions of organellar zones created by membrane lipids

Research abstract

In eukaryotic cells, intracellular organelles are supposed to have various functional domains (“zones”). Phospholipids and sterols should contribute to the formation and the specific functions of the zones. In this project, we identify new organellar zones, analyze the components of the zones, clarify the molecular mechanisms underlying the formation of the zones, and investigate the cellular functions of the zones.

Original papers

1. Akagi, S., Kono, N., Ariyama, H., Shindou, H., Shimizu, T. and Arai, H. (2016) Lysophosphatidylcholine acyltransferase 1 protects against cytotoxicity induced by polyunsaturated fatty acids. FASEB J 30, 2027-2039
2. Mukai, K., Konno, H., Akiba, T., Uemura, T., Waguri, S., Kobayashi, T., Barber, G. N., Arai, H. and Taguchi, T. (2016) Activation of STING requires palmitoylation at the Golgi. Nat Commun 7, 11932
3. Lee, S., Uchida, Y., Wang, J., Matsudaira, T., Nakagawa, T., Kishimoto, T., Mukai, K., Inaba, T., Kobayashi, T., Molday, R. S., Taguchi, T. and Arai, H. (2015) Transport through recycling endosomes requires EHD1 recruitment by a phosphatidylserine translocase. EMBO J 34, 669-688
4. Maekawa, M., Terasaka, S., Mochizuki, Y., Kawai, K., Ikeda, Y., Araki, N., Skolnik, E. Y., Taguchi, T. and Arai, H. (2014) Sequential breakdown of 3-phosphorylated phosphoinositides is essential for the completion of macropinocytosis. Proc Natl Acad Sci U S A 111, E978-87
5. Ohba, Y., Sakuragi, T., Kage-Nakadai, E., Tomioka, N. H., Kono, N., Imae, R., Inoue, A., Aoki, J., Ishihara, N., Inoue, T., Mitani, S. and Arai, H. (2013) Mitochondria-type GPAT is required for mitochondrial fusion. EMBO J 32, 1265-1279
6. Kono, N., Ohto, U., Hiramatsu, T., Urabe, M., Uchida, Y., Satow, Y. and Arai, H. (2013) Impaired α-TTP-PIPs interaction underlies familial vitamin E deficiency. Science 340, 1106-1110
7. Uchida, Y., Hasegawa, J., Chinnapen, D., Inoue, T., Okazaki, S., Kato, R., Wakatsuki, S., Misaki, R., Koike, M., Uchiyama, Y., Iemura, S., Natsume, T., Kuwahara, R., Nakagawa, T., Nishikawa, K., Mukai, K., Miyoshi, E., Taniguchi, N., Sheff, D., Lencer, W. I., Taguchi, T. and Arai, H. (2011) Intracellular phosphatidylserine is essential for retrograde membrane traffic through endosomes. Proc Natl Acad Sci U S A 108, 15846-15851
8. Imae, R., Inoue, T., Kimura, M., Kanamori, T., Tomioka, N. H., Kage-Nakadai, E., Mitani, S. and Arai, H. (2010) Intracellular phospholipase A1 and acyltransferase, which are involved in Caenorhabditis elegans stem cell divisions, determine the sn-1 fatty acyl chain of phosphatidylinositol. Mol Biol Cell 21, 3114-3124
9. Lee, H. C., Inoue, T., Imae, R., Kono, N., Shirae, S., Matsuda, S., Gengyo-Ando, K., Mitani, S. and Arai, H. (2008) Caenorhabditis elegans mboa-7, a member of the MBOAT family, is required for selective incorporation of polyunsaturated fatty acids into phosphatidylinositol. Mol Biol Cell 19, 1174-1184

Reviews

1. Kono, N. and Arai, H. (2015) Intracellular Platelet-Activating Factor Acetylhydrolase, Type II: A Unique Cellular Phospholipase A2 That Hydrolyzes Oxidatively Modified Phospholipids. Enzymes 38, 43-54
2. Hattori, M. and Arai, H. (2015) Intracellular PAF-Acetylhydrolase Type I. Enzymes 38, 23-36
3. Lee, S., Taguchi, T. and Arai, H. (2015) Endosomal lipid flippases and their related diseases. Channels (Austin) 9, 166-168
4. Kono, N. and Arai, H. (2014) [Regulation of the vitamin E level in the body by alpha-TTP, alpha-tocopherol-specific transfer protein]. Seikagaku 86, 232-241