臨床分析・分子生物学分野

臨床分析学（大川G）

  1. Futatsugi A., *Tozuka M., Horiuchi Y., Ohkawa R., and Kosho T., High-density lipoprotein functionality in cholesterol efflux in early childhood is related to the content ratio of triglyceride to cholesterol. 14(1): 23323, 2024. doi: 10.1038/s41598-024-74699-5.
  2. *Mikame M., Tsuno N.H., Miura Y., Kitazaki H., Uchimura D., Miyagi T., Miyazaki T., Onodera T., Ohashi W., Kameda T., Ohkawa R., Kino S., and Muroi K. Anti-A and anti-B antibody titers and age, gender, biochemical parameters, body mass index in Japanese blood donors (ABO antibody titers and other parameters). Immunohematology. 39(4):155-165, 2023. doi: 10.2478/immunohematology-2023-023.
  3. Yamazaki A., Nukui Y., Kameda T., Saito R., Koda Y., Ichimura N., Tohda S., and *Ohkawa R. Variation in presepsin and thrombomodulin levels for predicting COVID-19 mortality. Scientific Reports. 13(1): 21493, 2023. doi: 10.1038/s41598-023-48633-0.
  4. Lai S.J., Kameda T., Morita M., Yamagata Y., Nishizaka K., Horiuchi Y., Kobayashi Y., Usami Y., Liu J.J., Kasama T., Tozuka M., and *Ohkawa R. Characterization of novel truncated apolipoprotein A-I in human highdensity lipoprotein generated by sequential treatment with myeloperoxidase and chymase. Biochimie. 218: 34-45, 2023. Doi: 10.1016/j.biochi.2023.09.024.
  5. *Mikame M., Tsuno N.H., Miura Y., Kitazaki H., Uchimura D., Miyazaki T., Miyagi T., Onodera T., Ohashi W., Kameda T., Ohkawa R., Kino S., and Muroi K. Decreased rate of blood donors with high ABO antibody titers in Japan and the underlying factors: Comparisons between 2010 and 2021. Transfusion and Apheresis Science. 62(6): 103812, 2023. doi: 10.1016/j.transci.2023.103812.
  6. Miyakoshi T., Mutsuda Y., Horiuchi Y., Kameda T., Tozuka M., and *Ohkawa R. Improvement in bilirubin influence on cholesterol efflux capacity evaluation using the immobilized liposome-bound gel beads method. Bioscience Reports. 43(6):BSR20230393, 2023. doi: 10.1042/BSR20230393.
  7. Mutsuda Y., Miyakoshi T., Horiuchi Y., Kameda T., Tozuka M., and *Ohkawa R. Development and validation of novel automatable assay for cholesterol efflux capacity. Bioscience Reports. 43 (2): BSR20221519, 2023. doi.org/10.1042/BSR20221519.
  8. Ogino M., Kameda T., Mutsuda Y., Tanaka H., Takahashi J., Okazaki M., Ai M., and *Ohkawa R. Development of internal standard for lipoprotein subclass analysis using dual detection gel-permeation high-performance liquid chromatography system. Biosience Reports. 42(6):BSR20220291, 2022. doi: 10.1042/BSR20220291.
  9. Horiuchi Y., Lai S.J., Kameda T., Tozuka M., and *Ohkawa R. Novel cholesterol efflux assay using immobilized liposome-bound gel beads: Confirmation and improvement for application in clinical laboratory. Ann Clin Biochem. 59(2):134-143, 2022. DOI: 10.1177/00045632211054406.73.
  10. Kameda T., Horiuchi Y., Shimano S., Yano K., Lai S.J., Ichimura N., Tohda S., Kurihara Y., Tozuka M., *Ohkawa, R. Effect of myeloperoxidase oxidation and N-homocysteinylation of high-density lipoprotein on endothelial repair function. Biological Chemistry. 403(3):265-277, 2021. doi: 10.1515/hsz-2021-0247.
  11. Kobayashi, T., *Kurano, M., Nanya, M., Shimizu, T., Ohkawa, R., Tozuka, M., and Yatomi, Y. Glycation of HDL polymerizes Apolipoprotein M and attenuates its capacity to bind to sphingosine 1-phosphate. Journal of Atherosclerosis and Thrombosis. 28: 730-741, 2021. doi: 10.5551/jat.55699
  12. Yamazaki, A., *Ohkawa, R., Yamagata, Y., Horiuchi, Y., Lai, S.J., Kameda, T., Ichimura, N., Tohda, S. and Tozuka, M. Apolipoprotein C-II and C-III preferably transfer to both high-density lipoprotein (HDL)2 and the larger HDL3 from very low-density lipoprotein (VLDL). Biological Chemistry. 402(4): 439-449, 2021. doi: 10.1515/hsz-2020-0288
  13. Shimano S., *Ohkawa, R., Nambu M., Sasaoka M., Yamazaki A., Fujii Y., Horiuchi Y., Lai S.J., Kameda T., Ichimura N., Fujita K., Tohda S. and Tozuka M. Marked changes in serum amyloid A distribution and high-density lipoprotein structure during acute inflammation. Biomed Res Int. 2021:9241259, 2021. doi: 10.1155/2021/9241259. eCollection 2021
  14. *Iwata, Y., Kitajima, S., Yamahana, J., Shimomura, S., Yoneda-Nakagawa, S., Sakai, N., Furuichi, K., Ogura, H., Sato, K., Toyama, T., Yamamura, Y., Miyagawa, T., Hara, A., Shimizu, M., Ohkawa, R., Kurano, M., Yatomi, Y., and Wada, T. Higher serum levels of Autotaxin and Phosphatidylserine-Specific Phospholipase A1 in the Patients with Lupus Nephritis. International Journal of Rheumatic Diseases. 24(2): 231-239, 2021. doi: 10.1111/1756-185X.14031
  15. *Ohkawa, R., Low, H., Mukhamedova, N., Fu, Y., Lai, S.J., Sasaoka, M., Hara, A., Yamazaki, A., Kameda, T., Horiuchi, Y., Meikle, J.P., Pernes, G., Lancaster, G.I., Ditiatkovski, M., Nestel, P., Vaisman, B.L., Sviridov, De., Murphy, A.J., Remaley, A.T., *Sviridov, Dm., and Tozuka, M. Cholesterol transport between red blood cells and lipoproteins contributes to cholesterol metabolism in blood. J. Lipid Res. 61(12): 1577-1588, 2020. doi:10.1194/jlr.RA120000635
  16. Low H., Mukhamedova N., Capettini L.D.S.A., Xia Y., Carmichael I., Cody S.H., Huynh K., Ditiatkovski M., Ohkawa R., Bukrinsky M., Meikle P.J., Choi S.H, Field S., Miller Y.I., and *Sviridov D. Cholesterol Efflux-Independent Modification of Lipid Rafts by AIBP (Apolipoprotein A-I Binding Protein). Arterioscler Thromb Vasc Biol. 40(10): 2346-2359, 2020. doi: 10.1161/ATVBAHA.120.315037
  17. Horiuchi, Y., Lai S.J., Kameda, T., Tozuka, M., and *Ohkawa, R. Comparison of a novel cholesterol efflux assay using immobilized liposome-bound gel beads with the conventional method. Bioscience Reports. 40 (8) BSR20201495, 2020.
  18. Mukhamedova, N., Hoang, A., Dragoljevic, D., Dubrovsky, L., Pushkarsky, T., Low, H., Ditiatkovski, M., Fu, Y., Ohkawa R., Meikle, PJ., Horvath, A., Brichacek, B., Miller, YI., Murphy, A., Bukrinsky, M., and *Sviridov., D. Exosomes containing HIV protein Nef reorganize lipid rafts potentiating inflammatory response in bystander cells. PLOS Pathogens. 15(7):e1007907. doi: 10.1371/journal.ppat.1007907, 2019
  19. Lai SJ, *Ohkawa, R., Horiuchi Y., Kubota, T., and Tozuka M. Red Blood Cells Participate in Reverse Cholesterol Transport by Mediating Cholesterol Efflux of High-density lipoprotein and Apolipoprotein A-I from THP-1 macrophages. Biological Chemistry. 400(12): 1593-1602, 2019.
  20. Sato M., *Ohkawa R., Low H., Nishimori M., Okubo S., Yoshimoto A., Yano K., Kameda T., Yatomi Y., and Tozuka M. Serum amyloid A does not affect high-density lipoprotein cholesterol measurement by a homogeneous assay. Clin Biochem. 63: 97-101, 2019
  21. Horiuchi Y., *Ohkawa, R., Lai S.J., Yamazaki A., Ikoma H., Yano K., Kameda T., and Tozuka M. Characterization of the cholesterol efflux of apolipoprotein E-containing high-density lipoprotein in THP-1 cells. Biol Chem. 400(2): 209-218, 2019
  22. Horiuchi Y., *Ohkawa, R., Lai SJ, Shimano S, Hagihara M, Tohda S, Kameda T, and Tozuka M. Usefulness of apolipoprotein B-depleted serum in cholesterol efflux capacity assay using immobilized liposome-bound gel beads Bioscience Reports. 39 (4) BSR20190213; DOI:10.1042/BSR20190213, 2019
  23. Mishima Y., Kurano M., Kobayashi T., Nishikawa M., Ohkawa R., Tozuka M., and *Yatomi Y. Dihydro-sphingosine 1-phosphate interacts with carrier proteins in a manner distinct from that of sphingosine 1-phosphate. Bioscience Reports. 38(5). pii: BSR20181288. doi: 10.1042/BSR20181288, 2018
  24. Horiuchi Y, Lai SJ, Yamazaki A, Nakamura A, Ohkawa R, Yano K, Kameda T, Okubo S, Shimano S, Hagihara M, Tohda S, *Tozuka M. Validation and application of a novel cholesterol efflux assay using immobilized liposomes as a substitute for cultured cells. Biosci Rep. 2018 Mar 15. pii: BSR20180144. doi: 10.1042/BSR20180144.
  25. Ohkawa R, Kurano M, Sakai N, Kishimoto T, Nojiri T, Igarashi K, Hosogaya S, Ozaki Y, Dohi T, Miyauchi K, Daida H, Aoki J, Okubo S, Ikeda H, Tozuka M, *Yatomi Y. Measurement of plasma choline in acute coronary syndrome: importance of suitable sampling conditions for this assay. Sci Rep. 2018 Mar 16;8(1):4725. doi: 10.1038/s41598-018-23009-x.
  26. Kareinen I, Baumann M, Nguyen SD, Maaninka K, Anisimov A, Tozuka M, Jauhiainen M, Lee-Rueckert M, *Kovanen PT. Chymase released from hypoxia-activated cardiac mast cells cleaves human apolipoproteinA-I at Tyr192 and compromises its cardioprotective activity. J Lipid Res. 2018 Mar 26. pii: jlr.M077503. doi: 10.1194/jlr.M077503.
  27. Suzuki L, Hirayama S, Fukui M, Sasaki M, Hiroi S, Ayaori M, Terai S, Tozuka M, Watada H, *Miida T. Lipoprotein-X in cholestatic patients causes xanthomas and promotes foam cell formation in human macrophages. J Clin Lipidol, 11:110-118, 2017. doi: 10.1016/j.jacl.2016.10.013.
  28. Yano K, *Ohkawa R, Sato M, Yoshimoto A, Ichimura N, Kameda T, Kubota T, Tozuka M. Cholesterol efflux capacity of apolipoprotein A-I varies with the extent of differentiation and foam cell formation of THP-1 cells. J Lipids 2016, 2016/9891316.
  29. Yoshimoto A, *Ohkawa R, Yano K, Sato M, Ichimura N, Usami Y, Miyazaki A, Sugano M, Uehara T, Tozuka M. Paraoxonase 1 associated with high-density lipoprotein transfers to oxidized low-density lipoprotein depending on the degree of oxidation. Int J Anal Bio-Sci, 4: 99-109, 2016
  30. Sato M, *Ohkawa R, Yoshimoto A, Yano K, Ichimura N, Nishimori M, Okubo S,. Yatomi Y, Tozuka M. Effects of serum amyloid A on the structure and antioxidant ability of high-density lipoprotein. Biosci Rep 2016. doi: 10.1042/BSR20160075.
  31. Nagura Y, *Tsuno NH, Kano K, Inoue A, Aoki J, Hirowatari Y, Kaneko M, Kurano M, Matsuhashi M, Ohkawa R, Tozuka M, Yatomi Y, Okazaki H. Regulation of the lysophosphatidylserine and sphingosine 1-phosphate levels in autologous whole blood by the pre-strage leukocyte reduction. Transfus Med 2016, doi: 0.1111/tme.12326
  32. Ichimura N, Sato M, Yoshi moto A, Yano K, Ohkawa R, Kasama Takeshi, *Tozuka M, High-density lipoprotein binds to Mycobacterium avium and affects the infection of THP-1 macropage. J Lipids 2016, doi.org/10.1155/2016/4353620
  33. Shukuya K, Ogura S, Tokuhara Y, Okubo S, Yatomi Y, Tozuka M, *Shimosawa T. Novel round cells in urine sediment and their clinical implication. Clin Chem Acta 457: 142-149, 2016. doi: 10.1016/j.cca2016.04.017
  34. Nanya M, Sato M, Tanimoto K, Tozuka M, Mizutani S, *Takagi M. Dysregulation of the DNA damage response and KMT2A rearrangement in fetal liver cells. PLOS ONE 2015, doi:10.1371/journal.pone.0144540
  35. Kameda T, Ohkawa R, Yano K, Usami Y, Miyazaki A, Matsuda K, Kawasaki K, Sugano M, Kubota T, *Tozuka M. Effects of Myeloperoxidase-Induced Oxidation on Anti-Atherogenic Functions of High-Density Lipoprotein. J Lipids 2015, doi.org/10.1155/2015/592594
  36. Ohkawa R,Kurano M,Mishima Y,Nojiri T,Tokuhara Y,Kishimoto T,Nakamura K, Okubo S, Hosogaya S, Ozaki Y, Yokota H, Igarashi K, Ikeda H, Tozuka M, *Yatomi Y. Possible involvement of sphingomyelin in the regulation of the plasma sphingosine 1-phosphate level in human subjects. Clin Biochem 48: 690-697, 2015. doi: 10.1016/j.clinbiochem.2015.03.019.
  37. Iino J, Osada M, Kurano M, Kaneko M, Ohkawa R, Satoh Y, Okubo S, Ozaki Y, Tozuka M, Tsuno NH, *Yatomi Y. Platelet-derived sphingosine 1-phosphate induces migration of Jurkat cells. Lipid Health Dis 2014, doi:10.1186/1476-511X-13-150
  38. Miyazaki A, Sagae N, Usami　Y, Sato M, Kameda T, Yoshimoto A, Ishimine N, Matsuda K, Sugano M, Hara M, Honda H, *Tozuka M. N-homocysteinylation of apolipoprotein A-I impairs the protein’s antioxidant ability but not its cholesterol efflux capacity. Biol Chem 2014, doi: 10.1515/hsz-2013-0262
  39. Matsuhashi M, *Tsuno NH, Sone S, Mishima Y, Nagura Y, Watanabe-Okochi N, Ikeda T, Kashiwase K, Fukuda S, Iriyama T, Hyodo H, Yamashita T, Kamei Y, Arai S, Minami M, Fujii T, Kurokawa M, Tozuka M, Takahashi K, Santoso S. The role of alloantibodies against human platelet antigen-15 in multiply platelet transfused patients. Transfusion 2013 doi: 10.1111/trf.12455
  40. Matsuhashi M, *Tsuno NH, Ikeda T, Mishima Y, Watanabe-Okochi N, Santoso S, Tozuka M, Takahashi K. The frequencies of SLC44A2 alleles among the Japanese population. Tissue Antigens 81: 227-228, 2013
  41. Ono Y, Kurano M, Ohkawa R, Yokota H, Igarashi K, Aoki J, Tozuka M, *Yatomi Y. Sphingosine 1-phosphate release from platelets during clot formation: close correlation between platelet count and serum sphingosine 1-phosphate concentration. Lipid Health Dis 2013, 12:20. doi:10.1186/1476-511X-12-20
  42. Usami Y, Kobayashi Y, Kameda T, Miyazaki A, Matsuda K, Sugano M, Kawasaki K, Kurihara Y, Kasama T, *Tozuka M. Identification of sites in apolipoprotein A-I susceptible to chymase and carboxypeptidase A digestion. Biosci Rep 33: 49-56, 2013
  43. Kameda T, Usami Y, Shimada S, Haraguchi G, Matsuda K, Sugano M, Kurihara Y, Isobe M, *Tozuka M. Determination of Myeloperoxidase-induced apoAI-apoAII Heterodimers in High-Density Lipoprotein. Ann Clin Lab Sci 42: 384-391, 2012
  44. Matsuhashi M, *Tsuno NH, Kawabata M, Mishima Y, Okochi N, Santoso S, Tozuka M, Takahashi K. The frequencies of human neutrophil alloantigens among the Japanese population. Tissue Antigens 80: 336-340, 2012
  45. Usami Y, Matsuda K, Sugano M, Ishimine N, Kurihara Y, Sumida T, Yamauchi K, *Tozuka M, Detection of chymase-digested C-terminally truncated apolipoprotein A-I in normal human serum. J Immunol Methods 369: 51-58, 2011
  46. Ishimine N, Usami Y, Nogi S, Sumida T, Kurihara Y, Matsuda K, Nakamura K, Yamauchi K, Okumura N, *Tozuka M. Identification of N-homocysteinylated apolipoprotein AI in normal human serum. Ann Clin Biochem 47: 453-459, 2010

2022年度までの業績はこ ちら

2022年度までの業績はこ ちら

2022年度までの業績はこ ちら

分子生物学（鈴木G）

  1. Hayashi C, Suzuki N, Takahashi R, Akazawa C. Development of type I/II oligodendrocytes regulated by teneurin-4 in the murine spinal cord. Scientific Reports. 10(1):8611, 2020. doi: 10.1038/s41598-020-65485-0
  2. Hayashi C, Suzuki N, Mabuchi Y, Kikura N, Hosoda Y, de Vega S, Akazawa C. The extracellular domain of teneurin-4 promotes cell adhesion for oligodendrocyte differentiation. Biochemical and Biophysical Research Communications. 523(1):171-6, 2020. doi: 10.1016/j.bbrc.2019.12.002
  3. Suzuki N, Hyodo M, Hayashi C, Mabuchi Y, Sekimoto K, Onchi C, Sekiguchi K, Akazawa C. Laminin α2, α4, and α5 Chains Positively Regulate Migration and Survival of Oligodendrocyte Precursor Cells. Scientific Reports. 9(1):19882, 2019. doi: 10.1038/s41598-019-56488-7
  4. Ishii K, Sakurai H, Suzuki N, Mabuchi Y, Sekiya I, Sekiguchi K, Akazawa C. Recapitulation of Extracellular LAMININ Environment Maintains Stemness of Satellite Cells In Vitro. Stem Cell Reports. 10(2):568-582, 2018. doi: 10.1016/j.stemcr.2017.12.013
  5. Ogata Y, Mabuchi Y, Shinoda K, Horiike Y, Mizuno M, Otabe K, Suto EG, Suzuki N, Sekiya I, Akazawa C. Anterior cruciate ligament-derived mesenchymal stromal cells have a propensity to differentiate into the ligament lineage. Regenerative Therapy. 8:20-28, 2018. doi: 10.1016/j.reth.2017.12.001
  6. Suzuki N, Sekimoto K, Hayashi C, Mabuchi Y, Nakamura T, Akazawa C. Differentiation of Oligodendrocyte Precursor Cells from Sox10-Venus Mice to Oligodendrocytes and Astrocytes. Scientific Reports. 7(1):14133, 2017. doi: 10.1038/s41598-017-14207-0
  7. Ishii K, Suzuki N, Mabuchi Y, Sekiya I, Akazawa C. Technical advantage of recombinant collagenase for isolation of muscle stem cells. Regenerative Therapy. 7:1-7, 2017. doi: 10.1016/j.reth.2017.06.001
  8. Suto EG, Mabuchi Y, Suzuki N, Suzuki K, Ogata Y, Taguchi M, Muneta T, Sekiya I, Akazawa C. Prospectively isolated mesenchymal stem/stromal cells are enriched in the CD73+ population and exhibit efficacy after transplantation. Scientific Reports. 7(1):4838, 2017. doi: 10.1038/s41598-017-05099-1
  9. de Vega S, Hozumi K, Suzuki N, Nonaka R, Seo E, Takeda A, Ikeuchi T, Nomizu M, Yamada Y, Arikawa-Hirasawa E. Identification of Peptides Derived from the C-terminal Domain of Fibulin-7 Active for Endothelial Cell Adhesion and Tube Formation Disruption. Biopolymers. 106(2):184-195, 2016. doi: 10.1002/bip.22754
  10. Ogata Y, Mabuchi Y, Yoshida M, Suto EG, Suzuki N, Muneta T, Sekiya I, Akazawa C. Purified Human Synovium Mesenchymal Stem Cells as a Good Resource for Cartilage Regeneration. PLoS One. 10(6):e0129096, 2015. doi: 10.1371/journal.pone.0129096
  11. Ishii K, Suzuki N, Mabuchi Y, Ito N, Kikura N, Fukada S, Okano H, Takeda S, Akazawa C. Muscle Satellite Cell Protein Teneurin-4 Regulates Differentiation during Muscle Regeneration. Stem Cells. 33(10):3017-3027, 2015. doi: 10.1002/stem.2058
  12. Suto EG, Mabuchi Y, Suzuki N, Koyanagi A, Kawabata Y, Ozeki N, Nakagawa Y, Muneta T, Sekiya I, Akazawa C. High capacity of purified mesenchymal stem cells for cartilage regeneration. Inflammation and Regeneration. 35:78-85, 2015.
  13. Suzuki N, Mizuniwa C, Ishii K, Nakagawa Y, Tsuji K, Muneta T, Sekiya I, Akazawa C. Teneurin-4, a transmembrane protein, is a novel regulator that suppresses chondrogenic differentiation. Journal of Orthopaedic Research. 32(7):915-922, 2014. doi: 10.1002/jor.22616
  14. Suzuki N, Numakawa T, Chou J, de Vega S, Mizuniwa C, Sekimoto K, Adachi N, Kunugi H, Arikawa-Hirasawa E, Yamada Y, Akazawa C. Teneurin-4 promotes cellular protrusion formation and neurite outgrowth through focal adhesion kinase signaling. The FASEB Journal, 28(3):1386-1397, 2014. doi: 10.1096/fj.13-241034
  15. de Vega S, Suzuki N, Nonaka R, Sasaki T, Forcinito P, Arikawa-Hirasawa E, Yamada Y. A C-terminal fragment of fibulin-7 interacts with endothelial cells and inhibits their tube formation in culture. Archives of Biochemistry and Biophysics. 545:148-153, 2014. doi: 10.1016/j.abb.2014.01.013
  16. Suzuki R, Miyahara K, Murakami H, Doi T, Lane GJ, Mabuchi Y, Suzuki N, Yamataka A, Akazawa C. Abnormal neural crest innervation in Sox10-Venus mice with all-trans retinoic acid-induced anorectal malformations. Pediatric Surgery International. 30(2) 189-195, 2014. doi: 10.1007/s00383-013-3452-z
  17. Suzuki N, Fukushi M, Kosaki K, Doyle AD, de Vega S, Yoshizaki K, Akazawa C, Arikawa-Hirasawa E, Yamada Y. Teneurin-4 Is a Novel Regulator of Oligodendrocyte Differentiation and Myelination of Small-Diameter Axons in the CNS. The Journal of Neuroscience. 32(34):11586-11599, 2012. doi: 10.1523/JNEUROSCI.2045-11.2012
  1. Yamada M, Iwase M, Sasaki B, Suzuki N. The Molecular Regulation of Oligodendrocyte Development and CNS Myelination by ECM Proteins. Frontiers in Cell and Developmental Biology. 10:952135, 2022. doi: 10.3389/fcell.2022.952135
  2. 林千香子、山田桃奈、岩瀨未帆、鈴木喜晴. 髄鞘による軸索ホメオスタシス制御：遺伝子改変マウスの研究から. BIO Clinica. 36巻4号:63-67, 2021.
  3. 鈴木喜晴、岩瀨未帆、山田桃奈. 中枢神経系における髄鞘の軸索メンテナンス機構. BIO Clinica. 別冊9巻2号:103-109, 2020.
  4. Hayashi C, Suzuki N. Heterogeneity of Oligodendrocytes and Their Precursor Cells. Advances in Experimental Medicine and Biology. 1190:53-62, 2019. doi: 10.1007/978-981-32-9636-7_5
  5. 林千香子、鈴木喜晴. 中枢神経系の髄鞘形成におけるオリゴデンドロサイトの多様性. 生化学. 91巻5号:701-705, 2019. doi: 10.14952/SEIKAGAKU.2019.910701