top > Div. of Medical Devices > Dept. of Bioelectronics

  • Div. of Biomedical Materials
  • Div. of Biofunctional Restoration
  • Div. of Medical Devices
  • Div. of Biomolecular Chemistry
  • Medical and Dental Device Technology Incubation Center
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Member

Y. Miyahara Prof
A. Matsumoto Associate Prof
T. Goda Assistant Prof

Research Theme

An interdisciplinary area is explored and developed by integrating materials science and biological science. Novel biocompatible materials and functional bio-devices are developed for regenerative medicine, cell therapy, minimum-invasive surgery and clinical diagnostics. In the group of Yuji Miyahara, direct interaction between biomolecules and carriers in semiconductor materials has recently been investigated. For this purpose, an insulated gate field effect transistor (IGFET) has been used in combination with functional membranes. The FET is one of the most important and fundamental devices in the integrated circuit. In the case of FET type biochemical sensors, species to be detected and its selectivity can be determined by the materials coated on the surface of the gate insulator. Ion sensors, biosensors and oxygen sensor have been developed using polymer membranes, immobilized enzyme membranes and a solid electrolyte thin film, respectively. Recently, we have been investigating electrostatic detection of bio-molecular recognition using a biologically coupled field effect transistor (bio-FET). The principle of bio-FET is based on potentiometric detection of charge density change which is induced at a gate insulator/solution interface by specific bio-molecular recognition. In this scheme, the charge density change is directly transduced into electrical signal by the field effect. Based on this principle, various types of bio-FETs have been developed for detection of biomolecules. Research activities on biotransistors in our group can be categorized into the following research topics.

  1. Genetic analysis: Single base change could be detected using primer extension reaction on the gate of the FET. We found it possible to detect single base extension on the gate and successfully demonstrated single nucleotide polymorphism detection and DNA sequencing analysis.
  2. Carbohydrate detection: Carbohydrate detection is based on affinity between phenylboronic acid and carbohydrate. Self-assembled monolayer of phenylboronic acid was formed on the surface of a gold thin film. Potential response of the gold thin film was dependent on the sialic acid density at the cell membrane.
  3. Lipid bilayer detection: Uniform lipid bilayer is formed on the surface of the insulator of a field effect device by vesicle fusion. Charges of head groups of lipids can be controlled and detected as a shift of electrical characteristics of the field effect device. Mechanism of signal generation has been proposed.
  4. Protein and peptide analyses: The surface of a gold electrode was chemically modified with self-assembled monolayer of alkanethiol with different termini. Potentiometric signal for adsorption of proteins on the SAM layer was detected and investigated quantitatively. Density of adsorved proteins was calsulated and the sensitivity of the potentiometric detection was evaluated.
  5. Cell functional analysis: Electrical phenomena at the cell membrane can be monitored by the use of FET. By culturing an oocyte on the surface of the gate, kinetics of transporter-substrate interaction at cell membrane can be monitored non-invasively. Difference of transporting ability among genotypes of transporter could be discriminated using cell-based FETs.

Publications

  1. Tatsuro Goda and Yuji Miyahara, "Thermo-Responsive Molecular Switches for ATP using Hairpin DNA Aptamers", Biosens. Bioelectron., in press. DOI: 10.1016/j.bios.2011.02.041
  2. Alessandra Bonanni, Martin Pumera and Yuji Miyahara, "Influence of Gold Nanoparticle Size (2-50 nm) upon its Electrochemical Behavior: An Electrochemical Impedance Spectroscopic and Voltammetric Study", Phys. Chem. Chem. Phys., 2011, 13, 4980-4986. DOI: 10.1039/c0cp01209b
  3. Tatsuro Goda and Yuji Miyahara, "Molecularly Engineered Charge-Conversion of Proteins for Sensitive Biosensing", Anal. Chem., 2010, 82, 8946-8953. DOI: 10.1021/ac1018233
  4. Chiho Kataoka-Hamai, Mahoko Higuchi, Hideo Iwai and Yuji Miyahara, "Detergent-Mediated Formation of Polymer-Supported Phospholipid Bilayers", Langmuir, 2010, 26, 14600-14606. DOI: 10.1021/la102151p
  5. Akira Matsumoto, Horacio Cabral, Naoko Sato, Kazunori Kataoka, and Yuji Miyahara, "Assessment of tumor metastasis via direct determination of cell membrane sialic acid expression", Angew. Chem. Int. Ed., 2010, 49, 5494-5497. DOI: 10.1002/anie.201001220,Selected as 'Hot Paper'
  6. Alessandra Bonanni, Martin Pumera and Yuji Miyahara, "Rapid, Sensitive, and Label-Free Impedimetric Detection of a Single-Nucleotide Polymorphism Correlated to Kidney Disease", Anal. Chem., 2010, 82, 3772-3779. DOI: 10.1021/ac100165q
  7. Akira Matsumoto, Kazuya Yamamoto, Ryo Yoshida, Kazunori Kataoka, Takao Aoyagi, and Yuji Miyahara, "A totally synthetic glucose responsive gel operating in physiological aqueous conditions", Chem. Commun., 2010, 46, 2203-2205. DOI: 10.1039/b920319b,Selected as 'Front Cover'
  8. Tatsuro Goda and Yuji Miyahara, "Detection of Microenvironmental Changes Induced by Protein Adsorption onto Self-Assembled Monolayers using an Extended Gate-Field Effect Transistor", Anal. Chem., 2010, 82,1803-1810. DOI: 10.1021/ac902401y
  9. Chiho Kataoka-Hamai and Yuji Miyahara, "Mechanisms of Supported Bilayer Detection Using Field-Effect Devices", Analyst, 2010, 135, 189-194. DOI: 10.1039/b905197j
  10. Roberto Scipioni, Martin Pumera, Mauro Boero, Yuji Miyahara and Takahisa Ohno, "Investigation of the Mechanism of Adsorption of β-Nicotinamide Adenine Dinucleotide on Single-Walled Carbon Nanotubes", J. Phys. Chem. Lett., 2010, 1, 122-125. DOI: 10.1021/jz9000714
  11. John P Hulme, Seong Soo A. An, Nicholas Goddard, Yuji Miyahara and Akio Oki, "Fabrication of a flexible multi-referenced surface plasmon sensor using room temperature nanoimprint lithography", Current Applied Physics, 2009, 9, E185-E188. DOI: 10.1016/j.cap.2009.03.009
  12. Akira Matsumoto, Naoko Sato, Toshiya Sakata, Ryo Yoshida, Kazunori Kataoka, and Yuji Miyahara, "Chemical-to-Electrical-Signal Transduction Synchronized with Smart Gel Volume Phase Transition", Adv. Mater., 2009, 21, 4372-4378. DOI: 10.1002/adma.200900693
  13. Martin Pumera and Yuji Miyahara, "What amount of metallic impurities in carbon nanotubes is small enough not to dominate their redox properties?", Nanoscale, 2009, 1, 260-265. DOI: 10.1039/B9NR00071B,Highlighted in 'Chemical Science',The most downloaded article from Nanoscale in 2010.
  14. Martin Pumera, Hideo Iwai and Yuji Miyahara, "Germanium-oxide-coated Carbon Nanotubes", Nanotechnology, 2009, 20, 425606. DOI: 10.1088/0957-4484/20/42/425606
  15. Martin Pumera, Roberto Scipioni, Hideo Iwai, Takahisa Ohno, Yuji Miyahara, and Mauro Boero, "A Mechanism of Adsorption of b-Nicotinamide Adenine Dinucleotide on Graphene Sheets: Experiment and Theory", Chem. Eur. J., 2009, 15, 10851-10856. DOI: 10.1002/chem.200900399
  16. Akira Matsumoto, Takashi Endo, Ryo Yoshida and Yuji Miyahara, "Electrically visualized chemo-mechanical signal transduction by using a smart gel gate-modified field effect transistor", Chem. Commun., 2009, 5609-5611. DOI: 10.1039/b911689c
  17. Toshiya Sakata, Masaki Ihara, Izumi Makino, Yuji Miyahara, and Hiroshi Ueda, "Open sandwich-based immuno-transistor for label-free and noncompetitive detection of low molecular weight antigen", Anal. Chem. 2009, 81, 7532-7537. DOI: 10.1021/ac900457m
  18. Toshiya Sakata and Yuji Miyahara, "Capacitance-voltage measurement of transporter function at cell membrane", IEEJ Transaction on Sensors and Micromachines, 2009, 129, 242-244.
  19. Akira Matsumoto, Naoko Sato, Horacio Cabral, Kazunori Kataoka, and Yuji Miyahara, "Noninvasive Sialic Acid Detection at Cell Membrane by Using Phenylboronic Acid Modified Self-Assembled Monolayer Gold Electrode", J. Am. Chem. Soc., 2009, 131, 12022-12023. DOI: 10.1021/ja902964m
  20. Akira Matsumoto, Naoko Sato and Yuji Miyahara, "Label Free Carbohydrate Detection By Using Phenylboronic Acid Gate-Modified Field Effect Transistor",Curr. Appl. Phys. 2009, 9, 214-217. DOI: 10.1016/j.cap.2009.06.012
  21. Martin Pumera, Hideo Iwai, and Yuji Miyahara, "Bimetallic Nickel-Iron Impurities within Single-Walled Carbon Nanotubes Exhibit Redox Activity towards the Oxidation of Amino Acids", ChemPhysChem, 2009, 10, 1770 -1773. DOI: 10.1002/cphc.200900355
  22. Akira Matsumoto, Naoko Sato, Toshiya Sakata, Kazunori Kataoka and Yuji Miyahara, "Glucose-sensitive field effect transistor using totally synthetic compounds", J. Solid State Electrochem., 2009, 13, 165-170. DOI: 10.1007/s10008-008-0610-7
  23. Chiho Kataoka-Hamai, Yuji Miyahara, "Detection of supported lipid bilayers using their electric charge", Langmuir, 2008, 24, 9916-9920. DOI: 10.1021/la801623m
  24. Toshiya Sakata, Izumi Makino, Sayaka Kita and Yuji Miyahara, "Electrical detection of ovum membrane charges using biotransistor", Microelectronic Engineering, 2008, 85, 1337-1340. DOI: 10.1016/j.mee.2008.01.030
  25. Toshiya Sakata and Yuji Miyahara, "Noninvasive monitoring of transporter-substrate interaction at cell membrane", Anal. Chem., 2008, 80, 1493-1496. DOI: 10.1021/ac701977e
  26. Toshiya Sakata and Yuji Miyahara, "Detection of molecular charges at cell membrane", Japanese Journal of Applied Physics, 2008, 47, 368-370. DOI: 10.1143/JJAP.47.368
  27. Toshiya Sakata, Akio Ueda and Yuji Miyahara, "Cell adhesion characteristics of chemically modified silicon nitride surfaces", IEEJ Trans., 2007, 2, 295-300. DOI: 10.1143/JJAP.47.368
  28. Toshiya Sakata and Yuji Miyahara, Direct transduction of allele-specific primer extension into electrical signal using genetic field effect transistor", Biosens. Bioelectron., 2007, 22, 1311-1316. DOI: 10.1016/j.bios.2006.05.031
  29. Toshiya Sakata, Sumio Maruyama, Aiko Ueda, Hidenori Otsuka and Yuji Miyahara, "Stable immobilization of an oligonucleotide probe on a gold substrate using tripodal thiol derivatives", Langmuir, 2007, 23, 2269-2272. DOI: 10.1021/la0616193
  30. Toshiya Sakata and Yuji Miyahara, "DNA sequencing based on intrinsic molecular charges", Angew. Chem. Int. Edn., 2006, 45, 2225-2228. DOI: 10.1002/anie.200503154
  31. John P. Hulme, Jihye Gwak and Yuji Miyahara, "Bio-molecular embossing", J. Am. Chem. Soc., 2006, 128, 390-391. DOI: 10.1021/ja055805r
  32. Toshiya Sakata and Yuji Miyahara, "Detection of DNA recognition events using multi-well field effect devices", Biosens. Bioelectron., 2005, 21, 827-832. DOI: 10.1016/j.bios.2005.01.018
  33. Toshiya Sakata and Yuji Miyahara, "Potentiometric detection of single nucleotide polymorphism by using a genetic field effect transistor", ChemBioChem, 2005, 6, 703-710. DOI: 10.1002/cbic.200400253
  34. Toshiya Sakata, Masao Kamahori and Yuji Miyahara, "DNA analysis chip based on field effect transistors", Japanese Journal of Applied Physics, 2005, 44, 2854-2859. DOI: 10.1143/JJAP.44.2854
  35. Toshiya Sakata, Shinya Matsumoto, Yoshio Nakajima and Yuji Miyahara, "Potential behavior of bio-chemically modified gold electrode for extended gate field effect Transistor", Japanese Journal of Applied Physics, 2005, 44, 2860-2863. DOI: 10.1143/JJAP.44.2860
  36. Toshiya Sakata, Masao Kamahori and Yuji Miyahara, "Immobilization of oligonucleotide probes on Si3N4 surface and its application to genetic field effect transistor", Materials Science and Engineering C, 2004, 24, 827-832. DOI: 10.1016/j.msec.2004.08.042

Books and Review Papers

  1. C. Kataoka-Hamai, Y. Miyahara, "Field-effect detection using phospholipid membranes", Science and Technology of Advanced Materials, 2010, 11, 033001, DOI: 10.1088/1468-6996/11/3/033001
  2. A. Matsumoto, Y. Miyahara, Biodevices using semiconductor materials, K. Ariga ed. Fabrication and application of nano-space materials, Frontier publishing Co. (2009)
  3. A. Matsumoto, Y. Miyahara, Biodevices using soft-materials, J. Takahara, K. Kurihara, M. Maeda eds. Soft Matters, Maruzen, 222-232 (2009)
  4. A. Matsumoto, Y. Miyahara, Bio-molecular detection chip using biotransistors, K. Mitsubayashi ed. Advanced medical devices for healthcare and biomedicine, CMC publishing Co. 3-12(2009)
  5. Y. Miyahara, T. Sakata, and A. Matsumoto, Microbial Genetic Analysis Based on Field Effect Transistors, 14, 311-337 in: M. Zourob et al. (eds.), Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems, ? Springer Science+Business Media, LLC (2008)
  6. Y. Miyahara, T. Sakata, A. Matsumoto, Microbial Genetic Analyses Based on Field Effect Transistors、Principles od Bacterial Detection: Biosensors, Recognition Receptors and Microsystems, 313-339 (2008)
  7. Y. Miyahara, Detection of biomolecular recognition using biotransistors, Institute for Electrical Engineers of Japan ed. Flexible organic electronics, Ohmsha, 138-154 (2007)
  8. Y. Miyahara, Biotransistors, Advanced technology and application of biosensors, Techno-system, ,213-226 (2007)
  9. T. Sakata, A. Matsumoto, Y. Miyahara, Bio-transistor for Genetic Analysis、Functional materials, Vol.27, No. 5, 25-34 (2007)
  10. Y. Horiike, Y. Miyahara, Biochips and Biosensors, Kyoritsu publishing Co. (2006)
  11. Y. Miyahara, Ion-sensitive field effect transistors, T. Yamane, T. Matsunaga eds., Dictionary for nanobio-technology, Techno-system, 45-47 (2007)
  12. Y. Miyahara, DNA micro-fluidic system, I. Karube ed., Dictionary for biosensors and chemical sensors, Techno-system, 763-769 (2006)
  13. Y. Miyahara, Nanotechnology and clinical testing, J. of Medical technology, 50, 1334-1340 (2006)
  14. Y. Miyahara, T. Sakata, Electrical detection of biomolecules using field effect transistors, Surface Science, 27, 13-20 (2006)
  15. Y. Miyahara, Electrical detection of biomolecules using genetic transistors, Evaluation and application of organic transistor materials, CMC publishing Co., 169-179 (2005)
  16. Y. Miyahara, S. Maruyama, T. Sakata, Bioelectronics, Nanomaterials handbook, NTS publishing Co., 745-749 (2005)
  17. T. Sakata, Y. Miyahara, Sensitive detection of biomolecules using biotransistors, Ouyou Butsuri, 74, 1555-1562 (2005)
  18. Y. Miyahara, T. Sakata, Genetic analysis chip using field effect transistor, Chemical engineering, 50, 508-514 (2005)
  19. Y. Miyahara, Biochips using semiconductor technology, Materials Integration, 18, 50-54 (2005)
  20. Y. Miyahara, Semiconductor genetic analysis chip and its application to clinical diagnostics, Bioclinica, 20, 32-36 (2005)
  21. T. Sakata, Y. Miyahara, Application of MEMS technology to biochips, Bioscience and Industry, 62, 577-582 (2004)
  22. Y. Miyahara, Electrical detection of biomolecular recognition using genetic transistor, The Hitachi Scientific Instrument News, 47, 4175-4179 (2004)

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