Establish a New Frontier of Stem Cell Therapy by Connecting the Basic Science and Clinical Medicine.
We are working to uncover new diseases, elucidating the causes of disease and developing therapeutic modalities by connecting the knowledge and methodology of basic science such as immunology, molecular biology, cell biology and developmental engineering with clinical medicine. The Nakauchi lab is recruiting staff and researchers.
Current News
PublicationsList
Suchy F, Karigane D, Nakauchi Y, Higuchi M, Zhang J, Pekrun K, Hsu I, Fan A, Nichimura T, Charlesworth C, Bhadury J, Nichimura T, Wilkinson A, Kay M, Majeti R, Nakauchi H.
Genome engineering with Cas9 and AAV repair templates generates frequent concatemeric insertions of viral vectors.
Nat Biotechnol. 2024 Apr
doi: 10.1038/s41587-024-02171-w.
Nagano H, Mizuno N, Sato H, Mizutani E, Yanagida A, Kano M, Kasai M, Yamamoto H, Watanabe M, Suchy F, Masaki H, Nakauchi H.
Skin graft with dermis and appendages generated in vivo by cell competition.
Nat Commun. 2024 Apr
doi: 10.1038/s41467-024-47527-7.
Kinoshita S, Ishii M, Ando J, Kimura T, Yamaguchi T, Harada S, Takahashi F, Nakashima K, Nakazawa Y, Yamazaki S, Ohshima K, Takahashi K, Nakauchi H, Ando M.
Rejuvenated iPSC-derived GD2-directed CART Cells Harbor Robust Cytotoxicity Against Small Cell Lung Cancer.
Cancer Res Commun. 2024 Mar
doi: 10.1158/2767-9764.CRC-23-0259.
Okubo T, Rivron N, Kabata M, Masaki H, Kishimoto K, Semi K, Nakajima-Koyama M, kunitomi H, Kaswandy B, Sato H, Nakauchi H, Woltjen K, Saitou M, Sasaki E, Yamamoto T, Takashima Y.
Hypoblast from human pluripotent stem cells regulates epiblast development.
Nature. 2024 Feb
doi: 10.1038/s41586-023-06871-2.
Kano M, Mizuno N, Sato H, Kimura T, Hirochika R, Iwasaki Y, Inoshita N, Nagano H, Kasai M, Yamamoto H, Yamaguchi T, Suga H, Masaki H, Mizutani E, Nakauchi H.
Functional calcium-responsive parathyroid glands generated using single-step blastocyst complementation.
Proc Natl Acad Sci U S A. 2023 Jul. Epub 2023 Jun 28.
doi: 10.1073/pnas.2216564120.
Torikai K, Shimizu K, Nagatomo H, Kasai M, Kato-Itoh M, Kamada Y, Shibasaki I, Jeon H, Kikuchi R, Wakayama S, Suchy F, Nakauchi H, Wakayama T, Mizutani E.
Removal of sperm tail using trypsin and pre-activation of oocyte facilitates intracytoplasmic sperm injection in mice and rats.
J Reprod Dev. 2023 Feb
doi: 10.1262/jrd.2022-065.
Kano M, Mizutani E, Homma S, Masaki H, Nakauchi H.
Xenotransplantation and interspecies organogenesis: current status and issues.
Front Endocrinol (Lausanne). 2022 Aug
doi: 10.3389/fendo.2022.963282.
Yamazaki K, Kubara K, Ishii S, Li P, Dairiki R, Hihara T, Ishizuka Y, Izumi Y, Kumai M, Kamisako T, Ishizaki H, Sato H, Masaki H, Mizuno N, Mitsuhashi K, Ito M, Hamanaka S, Yamaguchi T, Watanabe M, Sugiyama F, Nakauchi H.
In vitro and in vivo functions of T cells produced in complemented thymi of chimeric mice generated by blastocyst complementation.
Sci Rep. 2022 Feb
Toral MA, Charlesworth CT, Ng B, Chemudupati T, Homma S, Nakauchi H, Bassuk AG, Porteus MH, Mahajan VB.
Investigation of Cas9 antibodies in the human eye.
Nat Commun. 2022 Feb
Oikawa M, Nagae M, Mizuno N, Iwatsuki K, Yoshida F, Inoue N, Uenoyama Y, Tsukamura H, Nakauchi H, Hirabayashi M, Kobayashi T.
Generation of Tfap2c-T2A-tdTomato knock-in reporter rats via adeno-associated virus-mediated efficient gene targeting.
Mol Reprod Dev. 2022 Feb
Harada S, Ando M, Ando J, Ishii M, Yamaguchi T, Yamazaki S, Toyota T, Ohara K, Ohtaka M, Nakanishi M, Shin C, Ota Y, Nakashima K, Ohshima K, Imai C, Nakazawa Y, Nakauchi H, Komatsu N.
Dual-antigen targeted iPSC-derived chimeric antigen receptor-T cell therapy for refractory lymphoma.
Mol Ther. 2022 Feb
Furukawa Y, Hamano Y, Shirane S, Kinoshita S, Azusawa Y, Ando J, Nakauchi H, Ando M.
Advances in Allogeneic Cancer Cell Therapy and Future Perspectives on "Off-the-Shelf" T Cell Therapy Using iPSC Technology and Gene Editing.
Cells. 2022 Jan
Watanabe M, Umeyama K, Nakano K, Matsunari H, Fukuda T, Matsumoto K, Tajiri S, Yamanaka S, Hasegawa K, Okamoto K, Uchikura A, Takayanagi S, Nagaya M, Yokoo T, Nakauchi H, Nagashima H.
Generation of heterozygous PKD1 mutant pigs exhibiting early-onset renal cyst formation.
Lab Invest. 2022 Jan
Kinoshita M, Kobayashi T, Planells B, Klisch D, Spindlow D, Masaki H, Bornelöv S, Stirparo GG, Matsunari H, Uchikura A, Lamas-Toranzo I, Nichols J, Nakauchi H, Nagashima H, Alberio R, Smith A.
Pluripotent stem cells related to embryonic disc exhibit common self-renewal requirements in diverse livestock species.
Development. 2021 Dec
Kobayashi T, Castillo-Venzor A, Penfold CA, Morgan M, Mizuno N, Tang WWC, Osada Y, Hirao M, Yoshida F, Sato H, Nakauchi H, Hirabayashi M, Surani MA.
Tracing the emergence of primordial germ cells from bilaminar disc rabbit embryos and pluripotent stem cells.
Cell Rep. 2021 Oct
Ishii M, Ando J, Yamazaki S, Toyota T, Ohara K, Furukawa Y, Suehara Y, Nakanishi M, Nakashima K, Ohshima K, Nakauchi H, Ando M.
iPSC-Derived Neoantigen-Specific CTL Therapy for Ewing Sarcoma.
Cancer Immunol Res. 2021 Oct
Harada S, Ando M, Ando J, Ishii M, Yamaguchi T, Yamazaki S, Toyota T, Ohara K, Ohtaka M, Nakanishi M, Shin C, Ota Y, Nakashima K, Ohshima K, Imai C, Nakazawa Y, Nakauchi H, Komatsu N
Dual-antigen targeted iPSC-derived chimeric antigen receptor-T cell therapy for refractory lymphoma.
Mol Ther. 2021 Oct
Sudo K, Yamazaki S, Wilkinson AC, Nakauchi H, Nakamura Y.
Polyvinyl alcohol hydrolysis rate and molecular weight influence human and murine HSC activity ex vivo.
Stem Cell Res. 2021 Oct
Bouchareychas L, Duong P, Phu TA, Alsop E, Meechoovet B, Reiman R, Ng M, Yamamoto R, Nakauchi H, Gasper WJ, Van Keuren-Jensen K, Raffai RL.
High glucose macrophage exosomes enhance atherosclerosis by driving cellular proliferation & hematopoiesis.
iScience. 2021 Jul
Nagaya M, Hasegawa K, Uchikura A, Nakano K, Watanabe M, Umeyama K, Matsunari H, Osafune K, Kobayashi E, Nakauchi H, Nagashima H.
Feasibility of large experimental animal models in testing novel therapeutic strategies for diabetes.
World J Diabetes. 2021 Apr
Kobayashi T, Goto T, Oikawa M, Sanbo M, Yoshida F, Terada R, Niizeki N, Kajitani N, Kazuki K, Kazuki Y, Hochi S, Nakauchi H, Surani MA, Hirabayashi M.
Blastocyst complementation using Prdm14-deficient rats enables efficient germline transmission and generation of functional mouse spermatids in rats.
Nat Commun. 2021 Feb
Wilkinson AC, Dever DP, Baik R, Camarena J, Hsu I, Charlesworth CT, Morita C, Nakauchi H, Porteus MH.
Cas9-AAV6 gene correction of beta-globin in autologous HSCs improves sickle cell disease erythropoiesis in mice.
Nat Commun. 2021 Jan
Nishimura T, Suchy FP, Bhadury J, Igarashi KJ, Charlesworth CT, Nakauchi H.
Generation of Functional Organs Using a Cell-Competitive Niche in Intra-and Inter-species Rodent Chimeras.
Cell Stem Cell. 2021 Jan
Nagaya M, Hasegawa K, Watanabe M, Nakano K, Okamoto K, Yamada T, Uchikura A, Osafune K, Yokota H, Nagaoka T, Matsunari H, Umeyama K, Kobayashi E, Nakauchi H, Nagashima H.
Genetically engineered pigs manifesting pancreatic agenesis with severe diabetes.
BMJ Open Diabetes Res Care. 2020 Nov
Yamamoto R, Nakauchi H.
In vivo clonal analysis of aging hematopoietic stem cells.
Mech Ageing Dev. 2020 Oct
Wilkinson AC, Igarashi KJ, Nakauchi H.
Haematopoietic stem cell self-renewal in vivo and ex vivo.
Nat Rev Genet. 2020 Sep
Honda T, Ando M, Ando J, Ishii M, Sakiyama Y, Ohara K, Toyota T, Ohtaka M, Masuda A, Terao Y, Nakanishi M, Nakauchi H, Komatsu N.
Sustainable Tumor-Suppressive Effect of iPSC-Derived Rejuvenated T Cells Targeting Cervical Cancers.
Mol Ther. 2020 Jul
Nishimura T, Xu H, Iwasaki M, Karigane D, Saavedra B, Takahashi Y, Suchy FP, Monobe S, Martin RM, Ohtaka M, Nakanishi M, Burrows SR, Cleary ML, Majeti R, Shibuya A, Nakauchi H.
Sufficiency for inducible Caspase-9 safety switch in human pluripotent stem cells and disease cells.
Gene Ther. 2020 Jul
Bouchareychas L, Duong P, Covarrubias S, Alsop E, Phu TA, Chung A, Gomes M, Wong D, Meechoovet B, Capili A, Yamamoto R, Nakauchi H, McManus MT, Carpenter S, Van Keuren-Jensen K, Raffai RL.
Macrophage Exosomes Resolve Atherosclerosis by Regulating Hematopoiesis and Inflammation via MicroRNA Cargo.
Cell Rep. 2020 Jul
Onodera S, Saito A, Hojo H, Nakamura T, Zujur D, Watanabe K, Morita N, Hasegawa D, Masaki H,Nakauchi H, Nomura T, Shibahara T, Yamaguchi A, Chung UI, Azuma T, Ohba S.
Hedgehog Activation Regulates Human Osteoblastogenesis.
Stem Cell Reports. 2020 Jul
Yamamoto R, Wilkinson AC, Nakauchi H.
In vivo and ex vivo haematopoietic stem cell expansion.
Curr Opin Hematol. 2020 Jul
Wilkinson AC, Nakauchi H.
Stabilizing hematopoietic stem cells in vitro.
Curr Opin Genet Dev. 2020 Jun
Wilkinson AC, Igarashi KJ, Nakauchi H.
Haematopoietic stem cell self-renewal in vivo and ex vivo.
Nat Rev Genet. 2020 May
Sato A, Kakinuma S, Miyoshi M, Kamiya A, Tsunoda T, Kaneko S, Tsuchiya J, Shimizu T, Takeichi E, Nitta S, Kawai-Kitahata F, Murakawa M, Itsui Y, Nakagawa M, Azuma S, Koshikawa N, Seiki M, Nakauchi H, Asahina Y, Watanabe M.
Vasoactive Intestinal Peptide Derived From Liver Mesenchymal Cells Mediates Tight Junction Assembly in Mouse Intrahepatic Bile Ducts.
Hepatol Commun. 2020 Feb
Kobayashi T, Kobayashi H, Goto T, Takashima T, Oikawa M, Ikeda H, Terada R, Yoshida F, Sanbo M, Nakauchi H, Kurimoto K, Hirabayashi M.
Germline development in rat revealed by visualization and deletion of Prdm14.
Development. 2020 Jan
Zujur D, Kanke K, Onodera S, Tani S, Lai J, Azuma T, Xin X, Lichtler AC, Rowe DW, Saito T, Tanaka S, Masaki H, Nakauchi H, Chung UI, Hojo H, Ohba S.
Stepwise strategy for generating osteoblasts from human pluripotent stem cells under fully defined xeno-free conditions with small-molecule inducers.
Regen Ther. 2020 Jan
Wilkinson AC, Ishida R, Nakauchi H, Yamazaki S.
Long-term ex vivo expansion of mouse hematopoietic stem cells.
Nat Protoc. 2020 Jan
Matsunari H, Watanabe M, Hasegawa K, Uchikura A, Nakano K, Umeyama K, Masaki H, Hamanaka S, Yamaguchi T, Nagaya M, Nishinakamura R, Nakauchi H, Nagashima H.
Compensation of Disabled Organogeneses in Genetically Modified Pig Fetuses by Blastocyst Complementation.
Stem Cell Reports. 2020 Jan
