Three Dimensional Tissue Regeneration Through Multipoint Molecular Weak Association

New methodology using ssDNA-PEG-lipid as a cell adhesive will be presented to display cells in certain area on a glass plate and to regenerate 3D tissue using cells as building blocks. When a ssDNA-PEG-lipid solution is added to a cell suspension, the hydrophobic alkyl chains of the ssDNA-PEG-lipid spontaneously form hydrophobic interactions with the lipid bilayer of the cell membrane. The ssDNA which is presented on the cell surface can be used as an adhesive to immobilize cells on various surfaces.

Various patterns can be drawn on glass plates using cells along the patterned aaDNA. Solutions of ssDNA-SHs were printed by an inkjet printer onto a glass plate covered with a gold thin layer. ssDNA-SHs were immobilized onto the surface through the thiol–gold interaction. Cells with ssDNA’-PEG-lipid, in which ssDNA’ was complementary to the ssDNA sequence of the ssDNA-SHs on the surface, were applied to the surface to induce cell attachment through the ssDNA–ssDNA’ hybridization.

The ssDNA-PEG-lipid was utilized to immobilize cells on a cell aggregate. ssDNA was introduced onto the surface of HEK293 cells with an ssDNA-PEG-lipid, and ssDNA’ was introduced onto the surface of cell aggregates with an ssDNA’-PEG-lipid. Then, the ssDNA’-cell aggregate were mixed with the ssDNA-HEK293 cells. The HEK293 cells were immobilized on the cell aggregate surface through DNA hybridization. Although the HEK293 cells existed as single cells on the aggregate just after immobilization, the surface of the aggregate was completely covered with a cell layer after 3 days in culture.

Publications

Nakaji-Hirabayashi T, Kato K, Iwata H., Improvement of neural stem cell survival in collagen hydrogels by incorporating laminin-derived cell adhesive polypeptides. Bioconjug Chem. 2012 Feb 15; 23(2):212-21.
Arima Y, Toda M, Iwata H., Surface plasmon resonance in monitoring of complement activation on biomaterials. Adv Drug Deliv Rev. 2011 Sep 16;63(12):988-99. Review.