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| Anatomy and physiolgy in the genetically manipulated mice that have visualyzed longitudinal stripes in the cerebellum | |||
| Aldolase is an enzyme involved in one of the essential steps in catalyzing
glucose. There are three isozymes, aldolases A, B and C, which are expressed
predominantly in muscle and brain, liver, and brain, respectively. Among
them, aldolase C is expressed in distinct subpopulations of cerebellar
Purkinje cells that are arranged in longitudinal stripe-shaped compartments
in the rodent and other mammalian cerebellar cortex. These stripes, we
think, represent basic structure of the cerebellar cortex. To facilitate
physiological and anatomical studies on this structure, we generated a
knock-in mouse strain in which fluorescent protein Venus is expressed in
place of aldolase C (Aldoc-Venus mouse). Projection patterns of Purkinje cell axons and climbing fiber axons have been already shown for aldolase C stripes in the cerebellar cortex. Therefore, one can has knowledge of axonal projection patterns for any given areas in Aldoc-Venus mice without making tracer injections. In cerebellar sections of Aldoc-Venus mice, the striped pattern of aldolase C (Aldoc) is clearly observed without immunostaining. Therefore, we can analyse detailed striped expression pattern of aldolase C in these mice. In Aldoc-Venus mice, the striped pattern is clearly visible during surgery under anaesthesia. So, we can record from identified stripes and inject tracers in indentified stripes to label mossy fibers retrogradely . |
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| Aldoc-Venus mouse cerebellum after perfusion-fixation （Whole mount and section） | |||
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| Aldoc-Venus mouse, cerebellar surface during surgery under anaesthesia (bright field and epifluorescence) | |||
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| Director: Izumi Sugihara ［Details］ | |||
| Publications: Nguyen-Minh VT, Tran-Anh K, Luo Y, Sugihara I (2019) Electrophysiological excitability and parallel fiber synaptic properties of zebrin-positive and -negative Purkinje cells in lobule VIII of the mouse cerebellar slice. Front Cell Neurosci 12:513. doi: 10.3389/fncel.2018.00513. DOWNLOAD Fujita H, Aoki H, Ajioka I, Yamazaki M , Abe M , Oh-Nishi A, Sakimura K, Sugihara I (2014) Detailed expression pattern of aldolase C (Aldoc) in the cerebellum, retina and other areas of the CNS studied in Aldoc-Venus knock-in mice, PLoS ONE, 9(1):e86679. DOWNLOAD. |
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