Klionsky, D.J., Abdalla, F. C. Tsubata, T. et al. (2012): Guidelines for the use and
 interpretation of assays for monitoring autophagy. Autophagy 8: 1-100.

Kishi, Y., Higuchi, T., Phoon, S., Kamiya, K., Riemekasten, G., Akiyoshi, K., Weigert, M. 
and Tsubata, T. (2012): Apoptotic marginal zone deletion of anti-Sm/ribonucleoprotein 
B cells. Proc. Natl. Acad. Sci. USA. 109(20) : 7811-6

Tsubata T.(2012): Role Of Inhibitory Bcr Co-Receptors In Immunity. Infect Disord
 Drug Targets. Mar 6.


Megesh, S., Ando, H., Tsubata, T., Ishida, H., Kiso, M. (2011) High-Affinity Ligands of 
Siglec Receptors and their Therapeutic Potentials. Current Medicinal Chemistry 18:

Poe, J. C., Smith , S. H., Haas, K. M., Yanabe, K., Tsubata, T., Matsushita, T. and 
Tedder, T. F. (2011) Amplified B lymphocyte CD40 signaling drives regulatory B10 cell 
expansion in mice. PLoS ONE 6: 22464

Tsubata, T. (2011) Role of inhibitory BCR co-receptors in immunity. Infectious 
Disorders - Drug Targets (in press).

Abdu-Allah, H. H. M, Watanabe, K., Kanie, O., Tsubata, T. Ishida, H. and Kiso, M.
(2011). Design and synthesis of multivalent heterobifunctional CD22-ligand as a 
potential immunomodulator. Synthesis (in press).

Watanabe, K., Tsuchiya, Y., Kawaguchi, Y., Sawada, S.-i., Ayame, H., Akiyoshi, K. and
 Tsubata, T. (2011): The use of cationic nanogels to deliver proteins to myeloma cells 
and primary T lymphocytes that poorly express heparan sulfate. Biomaterials. 32: 5900

Abdu-Allah, H. H. M., Watanabe, K., Completo, G. C., Sadagopan, M., Hayashizaki, K., 
Takaku, C., Tamanaka, T., Takematsu, H., Kozutsumi, Y., Paulson, J.C., Tsubata, T., 
Ando, H., Ishida, H. and Kiso, M. (2011): CD22-antagonists with nanomolar potency: 
The synergistic effect of hydrophobic groups at C-2 and C-9 of sialic acid scaffold. 
Bioorg. Med. Chem. 19: 1966-1971.

Bolduc, A., Long, E., Stapler, D., Cascalho, M., Tsubata, T. Koni, P. A. and Shimoda, 
M. (2010): Constitutive CD40L expression on B cells prematurely terminates germinal 
center response and leads to augmented plasma cell production in T cell areas. J. 
Immunol. 185: 220-230.

Kishi, Y., Aiba, Y., Higuchi, T., Furukawa, K., Tokuhisa, T., Takemori, T. and Tsubata, T. 
(2010): Augmented antibody response with premature germinal center regression in 
CD40L-transgenic mice. J. Immunol. 185: 211-219.

Ishiura, N., Nakashima, H., Watanabe, R., Kuwano, Y., Adachi, T., Takahashi, Y., 
Tsubata, T., Okochi, H., Tamaki, K., Tedder, TF., Fujimoto M.(2010): Differential 
phosphorylation of functional tyrosines in CD19 modulates B lymphocyte activation. 
Eur J Immunol. 40: 1192-1204.

Man, R.-Y., Onodera, T., Komatsu, E., Tsubata, T. (2010): Augmented B lymphocyte 
response to antigen in the absence of antigen-induced B lymphocyte signaling in an 
IgG-transgenic mouse line. Plos one. 5:8815.

 Abdu-Allah, H. H. M., Watanabe, K., Hayashizaki, K., Takaku, C., Tamanaka, T., 
Takematsu, H., Kozutsumi, Y., Tsubata, T., Ishida, H. and Kiso, M. (2009): Potent small 
molecule mouse CD22 inhibitors: exploring the interaction of the residue at C-2 of 
sialic acid scaffold. Bioorg. Med. Chem. Lett. 19:5573-75.
Abdu-Allah, H. H. M., Watanabe, K., Hayashizaki, K., Iwayama, Y., Takematsu, H.,
Kozutsumi, Y., Tsubata, T., Ishida, H. and Kiso, M (2009): Synthesis of biotinylated 
sialoside to probe CD22-ligand interactions. Tetrahedron Lett. 50:4488−91.
 Engels, N., Konig, L. M., Heemann, C., Lutz, J., Tsubata, T., Griep, S. and Wienands, 
J. (2009): The immunoglobulin tail tyrosine of surface IgG and IgE provides antigen 
receptor-intrinsic costimulation to class-switched B cells. Nature Immunol. 
Yanaba, K., Bouaziz, J.-D., Matsushita, T., Tsubata, T., and Tedder, T. F. (2009): The 
development and function of regulatory B cells expressing IL-10 requires antigen 
receptor diversity and TLR signals. J. Immunol. 182:7459-72.
Watanabe, K and Tsubata, T. (2009): Autophagy connects antigen receptor signaling 
to costimulatory signaling in B lymphocytes. Autophagy.5: 108-110.

Toda, M., Hisano, R., Yurugi, H., Akita, K., Maruyama, K., Inoue, M., Adachi, T., 
Tsubata, T. and Nakada, H. (2009): Ligation of tumor-produced mucins to CD22
 dramatically impairs splenic marginal zone B cells. Biochem J. 417:673-683.

Hou, R., Ohtsuji, M., Ohtsuji, N., Zhang, L., Adachi, T., Hirose, S. and Tsubata, T.
 (2009): The centromeric interval of chromosome 4 derived from C57BL/6 mice 
accelerates type 1 diabetes in NOD.CD72b congenic mice. Biochem. Biophys. Res. 
Comm. 380: 193-197.

Yan, B.-C., Adachi, T. and Tsubata, T.(2008): ER stress is involved in B cell antigen 
receptor ligation-induced apoptosis. Biochem. Biophys. Res. Comm. 365:143-148.

Onodera, T., Poe, J. C., Tedder, T. F. and Tsubata, T. (2008): CD22 regulates time
 course of both B cell division and antibody response. J. Immunol. 180: 907-913.

Zhu, C., Fujimoto, M., Sato, M., Yanagisawa, T. and Tsubata, T. (2008): Novel binding 
site for SH2-containing protein tyrosine phosphatase-1 in CD22 activated by B
 Lymphocyte stimulation with antigen. J. Biol. Chem. 283: 1653-1659.

Watanabe, K., Ichinose, S., Hayashizaki, K. and Tsubata, T. (2008): Induction of 
autophagy by B cell antigen receptor stimulation and its inhibition by costimulation. 
Biochem. Biophys. Res. Comm. 374:274-281

Abdu-Allah, H. H. M., Tamanaka, T., Yu, J., Lu, Z., Sadagopan, M., Adachi. T., 
Tsubata, T., Kelm, S., Ishida, H. and Kiso, M. (2008): Design, synthesis, and structure-
activity relationships of novel series of sialosides as CD22-specific inhibitors. J. 
Medicinal Chem. 51: 6665-6681.

Tsubata, T. (2008): Siglec-2 is a key molecule for immune response. Experimental 
Glycoscience, Glycobiology. Springer. p.167-170.

Adachi, T., Tsubata, T., (2008): FRET-based Ca2+ measurement in B lymphocyte by
 flow cytometry and confocal microscopy. Biochem Biophys Res Commun. 367:377-82

Watanabe, K and Tsubata, T. (2008): Autophagy connects antigen receptor signaling 
to costimulatory signaling in B lymphocytes. Autophagy. 

Sato, M., Adachi, T. and Tsubata, T. (2007): Augmentation of signaling through BCR 
containing IgE but not that containing IgA due to lack of CD22-mediated signal 
regulation. J. Immunol. 178: 2901-2907. 

Yu, J., Sawada, T., Adachi, T., Gao, X., Takematsu, H., Kozutsumi, Y., Ishida, H., Kiso, 
M. and Tsubata, T. (2007): Synthetic glycan ligand excludes CD22 from antigen 
receptor-containing lipid rafts. Biochem. Biophys. Res. Comm. 360: 759-764

Adachi, T., Wienands, J., Tsubata, T. and Kurosaki, T. (2007): Interdomain A is 
crucial for ITAM-dependent and -independent regulation of Syk. Biochem. Biophys.
 Res. Comm. 364: 111-7

Devi, S. S., Hagiyama, H., Adachi, T., Miyasaka, N. and Tsubata, T. (2006): The tumor 
suppressor p53 is not required for antigen receptor-mediated apoptosis of B 
lymphocytes. Signal Transduction 6: 54-61.

Akagawa, M., Ito, S., Toyoda, K., Ishii, Y., Tatsuda, E., Shibata, T., Kawai, Y., Ishino, K., 
Kishi, Y., Adachi, T., Tsubata, T., Takasaki, Y., Hattori, N., Matsuda, T. and Uchida, K. 
(2006): Bispecific antibodies against modified protein and DNA with oxidized lipids. 
Proc. Natl. Acad. Sci. USA. 103: 6160-5. 

Tsubata, T. (2006): B cell abnormality and systemic lupus erythematosus. APLAR 
J. Rheum. 9: 372-376

Tsubata, T. (2005): B cell abnormality and autoimmune disorders. Autoimmunity 38: 

Hirai, H., Adachi, T., and Tsubata, T. (2004): Involvement of cell cycle progression in 
survival signaling through CD40 in B lymphocyte line WEHI-231. Cell Death Differ. 11: 

Kawamura, T., Kanai, T., Dohi, T., Uraushihara, K., Totsuka, T., Iiyama, R., Taneda, C., 
Yamazaki, M., Nakamura, T., Higuchi, T., Aiba, Y., Tsubata, T. and Watanabe, M. (2004):
Ectopic CD40 ligand expression on B cells trigger intestinal inflammation. J. Immunol. 
172: 6388-6397.

Nitschke, L. and Tsubata, T. (2004): Molecular interactions regulate BCR signal 
inhibition by CD22 and CD72. Trends Immunol. 25: 543-550.

Suzuki, A, Kaisho, T., Ohishi, M., Tsukio-Yamaguchi, M., Tsubata, T., Koni, P. A., 
Sasaki, T., Mak, T. W., and Nakano, T. (2003): Critical roles of Pten in B cell 
homeostasis and immunoglobulin class switch recombination. J. Exp. Med. 197: 657-667.

Hokazono, Y., Adachi, T., Wabl, M., Tada, N., Amagasa, T., and Tsubata, T. (2003): 
Inhibitory co-receptors activated by antigens but not by anti-immunoglobulin heavy 
chain antibodies install requirement of co-stimulation through CD40 for survival and 
proliferation of B cells. J. Immunol. 171: 1835-1843.


● Wakabayashi, C., Adachi,T., Wienands,J., Tsubata,T.A distinct signaling pathway used by
the IgG-containing B cell antigen receptorScience.(2002) 298:2392-2395


論文別冊はこちら をクリック

● Higuchi, T., Aiba, Y., Nomura, T., Matsuda, J., Mochida, K., Suzuki, M., Kikutani, H., Honjo, T.,
Nishioka, K. and Tsubata T.Cutting Edge: Ectopic expression of CD40 ligand on B cells
induces lupus-like autoimmune disease. J. Immunol.(2002) 168:9-12.

●Tsubata, T.Molecular mechanisms for apoptosis induced by siganling through the B cell
antigen receptor.Int. Rev. Immunol.(2001) vol20, no.6, 675-678,791-803.

●Adachi, T., Wienands, J., Wakabayashi, C., Yakura, H., Reth, M. and Tsubata, T.SHP-1
requires inhibitory co-receptors to down-modulate B cell antigen receptor-mediated
phosphorylation of cellular substrates.J. Biol. Chem.276:26648-26655 (2001) .

●Suzuki, A., Tsukio-Yamaguchi, M., Ohteki, T., Sasaki, T., Kaisho, T., Kimura, Y., Yoshida, R.,
Wakeham, A., Higuchi, T., Fukumoto, M., Tsubata, T., Ohashi, P., Koyasu, S., Penninger, J. M.,
Nakano, T. and Mak, T. W.T cell-specific loss of Pten leads to defects in central and
peripheral tolerance. Immunity 14: 523-534 (2001)

●Tsubata, T., Wakabayashi, C. and Adachi, T.Regulation of B cell antigen receptor signaling
by CD72.In メActivating and Inhibitory Immunoglobulin-like Receptorsモ(ed. by Max D. Cooper,
Toshiyuki Takai, and Jeffrey V. Ravetch) Sprnger-Verlag Tokyo p. 123-128 (2001)

●Nagaoka, H., Takahashi, Y., Hayashi, R., Nakamura, T., Ishii, K., Matsuda, J.-I., Ogura,A.,
Shirakata, Y., Karasuyama, H., Sudo, T., Nishikawa, S.-I., Tsubata, T., Mizuochi, T., Asano, T.,
Sakano, H., Takemori, T. Ras mediates effector pathways responsible for pre-B cell survival,
which is essential for the developmental progression to the the late pre-B cell stage.J. Exp.
Med. 192: 171-181(2000)

●Yoshida, T., Higuchi, T., Hagiyama, H., Strasser, A., Nishioka, K., and Tsubata, T. Rapid B
cell apoptosis induced by antigen receptor ligation does not require Fas (CD95/APO-1), the
adapter protein FADD/MORT1 or CrmA-sensitive caspases but is defective in both MRL-+/
+ and MRL-lpr/lpr mice. Int. Immunol. 12: 517-526 (2000)

●Tsubata, T., and Honjo, T. B cell tolerance and autoimmunity. Rev. Immunogenet. 2: 18-25

●Adachi, T., Wakabayashi, C., Nakayama, T., Yakura, H., and Tsubata T. CD72 negatively
regulates signaling through the antigen receptor of B cells. J. Immunol. 164: 1223-1229

●Tsubata, T. Co-receptors on B lymphocytes. Curr. Opin. Immunol. 11: 249-255 (1999)

●Hagiyama, H., Adachi, T., Yoshida, T., Nomura, T, Miyasaka, N., Honjo, T., and Tsubata, T.
Signaling through the antigen receptor of B lymphocytes activates a p53-independent
pathway of c-Myc-induced apoptosis. Oncogene 18: 4091-4098 (1999)

●Tsubata, T. Apoptosis of mature B cells. Int. Rev. Immunol. 18: 347-365 (1999)

●Takeuchi, Y., Nishimura, K., Aoki, N., Adachi, T.,Sato, C., Kitajima, K., and Matsuda, T. A 42-
kDa glycoprotein from chicken egg-envelope, an avian homolog of the ZPC family
glycoproteins in mammalian Zona pellucida. Its first identification, cDNA cloning and
granulosa cell-specific expression. Eur. J. Biochm. 260: 736-742 (1999)

●Hirayama, F., Aiba, Y., Ikebuchi, K., Sekiguchi, S., and Ogawa, M. Differentiation in culture
of murine primitive lymphohematopoietic progenitors toward T-cell lineage.Blood. 93: 4187-
4195 (1999)

●Sato, T., Laver, J.H., Aiba, Y., and Ogawa, M.NK cell colony formation from human fetal
thymus. Exp. Hematol. 27: 726-733 (1999)

● Adachi, T., Flaswinkel, H., Yakura, H., Reth, M., and Tsubata, T. The B cell surface protein
CD72 recruits the tyrosine phosphatase SHP-1 upon tyrosine phosphorylation. In "
Proceedings of 10th International Congress of Immunology" (ed. by G.P.Talwar, I, Nath, N.K.
Ganguly, K.V.S.Rao) p.341-344. Monduzzi Editore S.p.A., Bologna. (1998)

●Adachi, T., Flaswinkel, H., Yakura, H., Reth, M., and Tsubata, T. The B cell surface protein
CD72 recruits the tyrosine phosphatase SHP-1 upon tyrosine phosphorylation. J. Immunol.
160: 4662-4665 (1998)

●Watanabe N., Nomura, T., Takai, T., Chiba, T., Honjo, T. and Tsubata, T. Antigen receptor
cross-linking by anti-immunoglobulin antibodies coupled to cell surface membrane induces
rapid apoptosis of normal spleen B cells. Scand. J. Immunol. 47: 541-547 (1998)

●Oubihi, M., Kitajima, K., Kobayashi, K., Adachi, T., Aoki, N., and Matsuda, T.Development of
an enzyme-linked immunosorbent assay-based method for measuring galactosyltransferase
activity using a synthetic glycopolymer acceptor substrate.Anal. Biochem. 257: 169-175

●Hotta, H., Aoki, N., Nakamura, R., Matsuda, T. and Adachi, T. Molecular analysis of a novel
protein kinase in maturing rice seed. Gene 212: 47-54 (1998)


鍔 田 武 志  Takeshii Tsubata

●Han, H., Nomura, T., Honjo, T., and Tsubata, T. 
Differential modulation of cyclin-dependent kinase inhibitor p27Kip1 by negative signaling via 
the antigen receptor of B cells and positive signaling via CD40. 
Eur. J. Immunol. 26: 2425-2432 (1996)

●Nomura, T., Han, H., Howard, M. C., Yagita, H., Yakura, H., Honjo, T., and Tsubata, T. 
Antigen receptor-mediated B cell death is blocked by signaling via CD72 or treatment with 
dextran sulfate and is defective in autoimmunity-prone mice.
Int. Immunol. 8: 867-875 (1996)

●Nisitani, S., Tsubata, T., Murakami, M., and Honjo, T.
Administration of interleukins 5 or 10 activates peritoneal B-1 cells and induces 
autoimmune hemolytic anemia in anti-erythrocyte autoantibody transgenic mice. 
Eur. J. Immunol. 25: 3047-3052 (1995) 

●Murakami, M., Tsubata, T., Shinkura, R., Nisitani, S., Okamoto, M., Yoshioka, H., Usui, T., 
Miyawaki, S. and Honjo, T.
Oral administration of lipopolysaccharides activates B-1 cells in the peritoneal cavity and 
lamina propria of the gut and induces autoimmune symptoms in an autoantibody transgenic 
J. Exp. Med. 180: 111-121 (1994) 

●Tsubata, T., Murakami, M. and Honjo, T.
Antigen-receptor cross-linking induces peritoneal B-cell apoptosis in normal but not 
autoimmunity-prone mice. 
Curr. Biol. 4: 8-17 (1994) 

●Nisitani, S., Tsubata, T., Murakami, M., Okamoto, M. and Honjo, T.
The bcl-2 gene product inhibits clonal deletion of self-reactive B lymphocytes in the 
periphery but not in the bone marrow. 
J. Exp. Med. 178: 1247-1254 (1993) 

●Tsubata, T., Wu, J. and Honjo, T. 
B-cell apoptosis induced by antigen receptor crosslinking is blocked by T-cell signal through 
Nature 364: 645-648 (1993)

●Murakami, M., Tsubata, T., Okamoto, M., Shimizu, A., Kumagai, S., Imura, H. and Honjo, T.
Antigen-induced apoptotic death of Ly-1 B cells responsible for autoimmune disease in 
transgenic mice. 
Nature 357: 77-80 (1992) 

●Tsubata, T. and Nishikawa, S.-I.
Molecular and cellular aspects of early B-cell development. 
Curr. Opin. Immunol. 3: 186-192 (1991)

●Tsubata, T. and Reth, M. 
The products of pre-B specific genes (l5 and VpreB) and the immunoglobulin m chain form a 
complex that is transported onto the cell surface. 
J. Exp. Med. 172: 973-976 (1990)

●Hombach, J., Tsubata, T., Leclercq, L., Stappert, H. and Reth, M. 
Molecular components of the B-cell antigen receptor complex of the IgM class. 
Nature 343: 760-762 (1990)