Six clones were obtained from each Plasmodium falciparum isolate, Gombak A (Malaysian), Bombak C (Malaysian), ST 9 (malaysian), ST 12 (Malaysian), ST 85 (Malaysian), ST 148 (Malaysian), Gambian (African) and TGR (Thailand) using electrophoretic dilution method. These clones were characterized by electrophoretic isoenzyme analysis of GPI (glucose phosphate isomerase) (EC. 5.3.1.9). Results showed that these were pure clones as they were monovariant with regards to GPI unlike their parent isolates which were divariant.

Key words: Plasmodium falciparum; isolate; clones; glucose phosphate isomerase; electrophretic variations.