Abstract
After the inoculation of Berenil into Tryponosoma gambiense infected mice, dyskinetoplastic forms appeared as a result of inhibition of kinetoplast duplication without any affect on nuclear and cytoplasmic duplication. The present study demonstrated that in some dividing forms with a nucleus appeared after the treatment with Berenil a flagellum in which the kinetoplast can not be seen near to it, was observed at the posterior end of the parasite and one kinetoplast with or without a flagellum migrated anteriorly. That is, a dividing form containing one kinetoplast, one nucleus and two flagella (1K1N2F), a dividing form containing one kinetoplast, one nucleus and a flagella (1K1N1F) were observed. In the latter, the kinetoplast without a flagellum migrated close to the nucleus or anteriorly far from it. Such trypanosomes appeared in the original clone after the treatment, but, not in the p-rosaniline resistant clone. We have never seen such migration of the kinetoplast without a flagellum in trypanosomes treated with various chemicals except for Berenil in the present study. The present study also indicated that the original clone was more sensitive than the p-rosaniline resistant clone to the effect of Berenil in inhibiting kinetoplast division.
Key words: Berenil; dyskinetoplastic form; kinetoplast migration; Trypanosoma gambiense.
Correspondence:Tadasuke Ono
Tadasuke Ono
Yoshihiro Ohnishi
Kazutoshi Takami
Miyuki Yamamoto
Department of Veterinary Epidemiology, College of Agriculture, University of Osaka Prefecture, Gakuen-cho 1-1, Sakai, Osaka 593, Japan.
Harison Chitambo
Akira Arakawa
Department of Veterinary Internal Medicine, College of Agriculture, University of Osaka Prefecture, Gakuen-cho 1-1, Sakai, Osaka 593, Japan.