Member

Endowed Research Division (Acellular Tissue and Regenerative Medical Material)

Research Theme

1. Regenerative medicine using decellularized biological tissue
   Decellularization is an attractive technique for preparing a scaffold in tissue engineering, as the decellularized tissues are essentially maintained the structure and physical properties of the living tissue. We have been developed a new decellularization method without detergents, high-hydrostatic pressure (HHP) method. Using this method, complete removal of infectious bacteria, viruses, and cells was accomplished.
2. Molecular aggregates formed by the high-hydrostatic pressure process
   Hydrogen bond assembles molecular assembly under high pressure. Using the HHP processing of more than 6,000 atmospheres, we try to prepare the nucleic acid assembly and apply them as gene delivery system.
3. Extracellular matrix remodeling
   We have developed an artificial extracellular matrix (ECM) that replicated the structure of the native ECM from a quaternary structure of collagen molecules using fibrillogenesis. We have studied about tissue remodeling using artificially reconstructed extracellular matrix. Specifically, we are investigating the applications of as artificial skin and artificial cornea of precision design artificial extracellular matrix structure.
4. Immune control system: technology of specific cell capture and release
   In cancer immunotherapy, by removing regulatory T cells (Treg) that negatively regulates immune reactions, to be able to enhance the anti-tumor immune responses have been revealed. We are developing technologies to capture and release Treg using interfacial science.

Publications

2015
1. M. Tabuchi, J. Negishi, A. Yamashita, T. Higami, A. Kishida, S. Funamoto. Effect of decellularized tissue powders on a rat model of acute myocardial infarction. Mater Sci Eng C, Mater Biol Appl, 56(1): 494-500, 2015.
2. Y. Hashimoto, S. Funamoto, S. Sasaki, J. Negishi, T. Honda, S. Hattori, K. Nam, T. Kimura, M. Mochizuki, H. Kobayashi, A. Kishida, Corneal regeneration by deep anterior lamellar keratoplasty (DALK) using decellularized corneal matrix. PLoS One, 10(7): e131989, 2015.
3. P. Wu, N. Nakamura, T. Kimura, K. Nam, T. Fujisato, S. Funamoto, T. Higami, A. Kishida. Decellularized porcine aortic intima-media as a potential cardiovascular biomaterial. Interact Cardiovasc Thorac Surg, 2015.
4. A. Matsuhashi, K. Nam, T. Kimura, A. Kishida. Fabrication of fibrillized collagen microspheres with the microstructure resembling an extracellular matrix. Soft matter, 11: 2844-2851, 2015.
5. Y. Suwa, K. Nam, K. Ozeki, T. Kimura, A. Kishida, T. Masuzawa. Thermal denaturation behavior of collagen fibrils in wet and dry environment. J Biomed Mater Res PartB, 2015.

2014
6. R. Matsushima, K. Nam, Y. Shimatsu, T. Kimura, T. Fujisato, A. Kishida. Decellularized dermis-polymer complex provides a platform for soft-to-hard tissue interfaces. Mater Sci Eng C, Mater Biol Appl, 35(1): 354-362, 2014.
7. E. G. Santoso, K. Yoshida, Y. Hirota, M. Aizawa, O. Yoshino, A. Kishida, Y. Osuga, S. Saito, T. Ushida, K. S. Furukawa. Application of detergents or high hydrostatic pressure. PLoS One, 9(7): e103201, 2014.
8. P. Wu, T. Kimura, H. Tadokoro, K. Nam, T. Fujisato, A. Kishida. Relation between the tissue structure and protein permeability of decellularized porcine aorta. Mater Sci Eng C, Mater Biol Appl, 43(1): 465-471, 2014.
9. Y. Nagaoka, H. Yamada, T. Kimura, A. Kishida, T. Fujisato, K. Takakuda. Reconstruction of small diameter arteries using decellularized vascular scaffolds. J Med Dent Sci, 61: 33-40, 2014.
10. J. Negishi, Y. Hashimoto, K. Nam, T. Kimura, S. Funamoto, A. Kishida. Application of a vacuum pressure impregnation technique for rehydrating decellularized tissues. Tissue Eng Part C, Methods, 20: 1-7, 2014.
11. K. Nam, T. Iwata, T. Kimura, H. Ikake, S. Shimizu, T. Masuzawa, A. Kishida. Adhesion between polymer surface modified by graft polymerization and tissue during surgery using an ultrasonically activated scalpel device. J Appl Polym Sci, 131(20): 1-8, 2014.
12. K. Nam, Y. Shimatsu, R. Matsushima, T. Kimura, A. Kishida. In-situ polymerization of PMMA inside decellularized dermis using UV photopolymerization. Eur Polym J, 60:163-171, 2014.
13. S. Nishino, A. Kishida, H. Yoshizawa, K. Shiomori. A protein extraction system with Water/Oil microemulsion formed by a biodegradable polymer surfactant solvent extraction research and development, Solvent Extr Res dev, Jpn, 21(1): 47-54, 2014.
14. J. Negishi, K. Nam, T. Kimura, Y. Hashimoto, S. Funamoto, T. Higami, T. Fujisato, A. Kishida. Fabrication of a heparin-PVA complex hydrogel for application as a vascular access. J Biomed Mater Res Part B, 102(7): 1426-1433, 2014.
15. T. Kimura, A. Okuno, Y. Ohya, A. Kishida. Assembly of Poly(vinyl alcohol) and DNA via hydrogen bonds induced by high hydrostatic pressurization. Sens Mater, 26(8): 607-614, 2014.
16. K. Nam, R. Matsushima, T. Kimura, T. Fujisato, A. Kishida. In vivo characterization of a decellularized dermis-polymer complex for use in percutaneous devices. Artif Organs, 38(12): 1060-1065, 2014.
17. K. Nam, J.-H. Seo, T. Kimura, N. Yui, A. Kishida. Relationships between molecular mobility, fibrillogenesis of collagen molecules, and inflammatory response: An experimental study in vitro and in vivo. J Colloid Interface Sci, 433(1): 16-25, 2014.
18. Y. Sawa, E. Tatsumi, T. Tsukiya, K. Matsuda, K. Fukunaga, A. Kishida, T. Masuzawa, G. Matsumiya, A. Myoui, M. Nishimura, T. Nishinaka, E. Okamoto, S. Tokunaga, T. Tomo, Y. Yagi, T. Yamaoka. 2013: the year in review Journal of Artificial Organs. J Artif Organs, 17(1): 1-8, 2014.

2013
19. N. Nakamura, K. Sugano, K. Nam, T. Kimura, T. Fujisato, A. Kishida. A basic study of osteogenesis between decellularized cortical bone pieces for bone graft construction. Adv Biomed Eng, 2: 95-100, 2013.
20. T. Kimura, Y. Kanda, A. Sano, K. Nam, Y. Sasaki, K. Akiyoshi, T. Fujisato, A. Kishida. High hydrostatic pressurized lipoplex enhances transfection efficiency in vitro. Adv Biomed Eng, 2: 80-83, 2013.
21. K. Nam, K. Yamamoto, K. Kasahara, T. Kimura, S. Funamoto, S. Shimizu, T. Higami, T. Masuzawa, A. Kishida. Fundamental study on the development of a surgical device for polymer-tissue adhesion using vibration damping of polymeric materials. J Appl Polym Sci, 130(4): 2532-2537, 2013.