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  1. Mutsuda Y., Miyakoshi T., Horiuchi Y., Kameda T., Tozuka M., and *Ohkawa R. Development and validation of novel automatable assay for cholesterol efflux capacity. Bioscience Reports. (in Press).
  2. Ogino M., Kameda T., Mutsuda Y., Tanaka H., Takahashi J., Okazaki M., Ai M., and *Ohkawa R. Development of internal standard for lipoprotein subclass analysis using dual detection gel-permeation high-performance liquid chromatography system. Biosience Reports. 42(6):BSR20220291, 2022. doi: 10.1042/BSR20220291.
  3. Horiuchi Y., Lai S.J., Kameda T., Tozuka M., and *Ohkawa R. Novel cholesterol efflux assay using immobilized liposome-bound gel beads: Confirmation and improvement for application in clinical laboratory. Ann Clin Biochem. 59(2):134-143, 2022. DOI: 10.1177/00045632211054406.73.   
  4. Kameda T., Horiuchi Y., Shimano S., Yano K., Lai S.J., Ichimura N., Tohda S., Kurihara Y., Tozuka M., *Ohkawa, R. Effect of myeloperoxidase oxidation and N-homocysteinylation of high-density lipoprotein on endothelial repair function. Biological Chemistry. 403(3):265-277, 2021. doi: 10.1515/hsz-2021-0247.
  5. Kobayashi, T., *Kurano, M., Nanya, M., Shimizu, T., Ohkawa, R., Tozuka, M., and Yatomi, Y. Glycation of HDL polymerizes Apolipoprotein M and attenuates its capacity to bind to sphingosine 1-phosphate. Journal of Atherosclerosis and Thrombosis. 28: 730-741, 2021. doi: 10.5551/jat.55699
  6. Yamazaki, A., *Ohkawa, R., Yamagata, Y., Horiuchi, Y., Lai, S.J., Kameda, T., Ichimura, N., Tohda, S. and Tozuka, M. Apolipoprotein C-II and C-III preferably transfer to both high-density lipoprotein (HDL)2 and the larger HDL3 from very low-density lipoprotein (VLDL). Biological Chemistry. 402(4): 439-449, 2021. doi: 10.1515/hsz-2020-0288
  7. Shimano S., *Ohkawa, R., Nambu M., Sasaoka M., Yamazaki A., Fujii Y., Horiuchi Y., Lai S.J., Kameda T., Ichimura N., Fujita K., Tohda S. and Tozuka M. Marked changes in serum amyloid A distribution and high-density lipoprotein structure during acute inflammation. Biomed Res Int. 2021:9241259, 2021. doi: 10.1155/2021/9241259. eCollection 2021
  8. *Iwata, Y., Kitajima, S., Yamahana, J., Shimomura, S., Yoneda-Nakagawa, S., Sakai, N., Furuichi, K., Ogura, H., Sato, K., Toyama, T., Yamamura, Y., Miyagawa, T., Hara, A., Shimizu, M., Ohkawa, R., Kurano, M., Yatomi, Y., and Wada, T. Higher serum levels of Autotaxin and Phosphatidylserine-Specific Phospholipase A1 in the Patients with Lupus Nephritis. International Journal of Rheumatic Diseases. 24(2): 231-239, 2021. doi: 10.1111/1756-185X.14031
  9. *Ohkawa, R., Low, H., Mukhamedova, N., Fu, Y., Lai, S.J., Sasaoka, M., Hara, A., Yamazaki, A., Kameda, T., Horiuchi, Y., Meikle, J.P., Pernes, G., Lancaster, G.I., Ditiatkovski, M., Nestel, P., Vaisman, B.L., Sviridov, De., Murphy, A.J., Remaley, A.T., *Sviridov, Dm., and Tozuka, M. Cholesterol transport between red blood cells and lipoproteins contributes to cholesterol metabolism in blood. J. Lipid Res. 61(12): 1577-1588, 2020. doi:10.1194/jlr.RA120000635
  10. Low H., Mukhamedova N., Capettini L.D.S.A., Xia Y., Carmichael I., Cody S.H., Huynh K., Ditiatkovski M., Ohkawa R., Bukrinsky M., Meikle P.J., Choi S.H, Field S., Miller Y.I., and *Sviridov D. Cholesterol Efflux-Independent Modification of Lipid Rafts by AIBP (Apolipoprotein A-I Binding Protein). Arterioscler Thromb Vasc Biol. 40(10): 2346-2359, 2020. doi: 10.1161/ATVBAHA.120.315037
  11. Horiuchi, Y., Lai S.J., Kameda, T., Tozuka, M., and *Ohkawa, R. Comparison of a novel cholesterol efflux assay using immobilized liposome-bound gel beads with the conventional method. Bioscience Reports. 40 (8) BSR20201495, 2020.
  12. Mukhamedova, N., Hoang, A., Dragoljevic, D., Dubrovsky, L., Pushkarsky, T., Low, H., Ditiatkovski, M., Fu, Y., Ohkawa R., Meikle, PJ., Horvath, A., Brichacek, B., Miller, YI., Murphy, A., Bukrinsky, M., and *Sviridov., D. Exosomes containing HIV protein Nef reorganize lipid rafts potentiating inflammatory response in bystander cells. PLOS Pathogens. 15(7):e1007907. doi: 10.1371/journal.ppat.1007907, 2019
  13. Lai SJ, *Ohkawa, R., Horiuchi Y., Kubota, T., and Tozuka M. Red Blood Cells Participate in Reverse Cholesterol Transport by Mediating Cholesterol Efflux of High-density lipoprotein and Apolipoprotein A-I from THP-1 macrophages. Biological Chemistry. 400(12): 1593-1602, 2019.
  14. Sato M., *Ohkawa R., Low H., Nishimori M., Okubo S., Yoshimoto A., Yano K., Kameda T., Yatomi Y., and Tozuka M. Serum amyloid A does not affect high-density lipoprotein cholesterol measurement by a homogeneous assay. Clin Biochem. 63: 97-101, 2019
  15. Horiuchi Y., *Ohkawa, R., Lai S.J., Yamazaki A., Ikoma H., Yano K., Kameda T., and Tozuka M. Characterization of the cholesterol efflux of apolipoprotein E-containing high-density lipoprotein in THP-1 cells. Biol Chem. 400(2): 209-218, 2019
  16. Horiuchi Y., *Ohkawa, R., Lai SJ, Shimano S, Hagihara M, Tohda S, Kameda T, and Tozuka M. Usefulness of apolipoprotein B-depleted serum in cholesterol efflux capacity assay using immobilized liposome-bound gel beads Bioscience Reports. 39 (4) BSR20190213; DOI:10.1042/BSR20190213, 2019
  17. Mishima Y., Kurano M., Kobayashi T., Nishikawa M., Ohkawa R., Tozuka M., and *Yatomi Y. Dihydro-sphingosine 1-phosphate interacts with carrier proteins in a manner distinct from that of sphingosine 1-phosphate. Bioscience Reports. 38(5). pii: BSR20181288. doi: 10.1042/BSR20181288, 2018
  18. Horiuchi Y, Lai SJ, Yamazaki A, Nakamura A, Ohkawa R, Yano K, Kameda T, Okubo S, Shimano S, Hagihara M, Tohda S, *Tozuka M. Validation and application of a novel cholesterol efflux assay using immobilized liposomes as a substitute for cultured cells. Biosci Rep. 2018 Mar 15. pii: BSR20180144. doi: 10.1042/BSR20180144.
  19. Ohkawa R, Kurano M, Sakai N, Kishimoto T, Nojiri T, Igarashi K, Hosogaya S, Ozaki Y, Dohi T, Miyauchi K, Daida H, Aoki J, Okubo S, Ikeda H, Tozuka M, *Yatomi Y. Measurement of plasma choline in acute coronary syndrome: importance of suitable sampling conditions for this assay. Sci Rep. 2018 Mar 16;8(1):4725. doi: 10.1038/s41598-018-23009-x.
  20. Kareinen I, Baumann M, Nguyen SD, Maaninka K, Anisimov A, Tozuka M, Jauhiainen M, Lee-Rueckert M, *Kovanen PT. Chymase released from hypoxia-activated cardiac mast cells cleaves human apolipoproteinA-I at Tyr192 and compromises its cardioprotective activity. J Lipid Res. 2018 Mar 26. pii: jlr.M077503. doi: 10.1194/jlr.M077503.
  21. Suzuki L, Hirayama S, Fukui M, Sasaki M, Hiroi S, Ayaori M, Terai S, Tozuka M, Watada H, *Miida T. Lipoprotein-X in cholestatic patients causes xanthomas and promotes foam cell formation in human macrophages. J Clin Lipidol, 11:110-118, 2017. doi: 10.1016/j.jacl.2016.10.013.
  22. Yano K, *Ohkawa R, Sato M, Yoshimoto A, Ichimura N, Kameda T, Kubota T, Tozuka M. Cholesterol efflux capacity of apolipoprotein A-I varies with the extent of differentiation and foam cell formation of THP-1 cells. J Lipids 2016, 2016/9891316.
  23. Yoshimoto A, *Ohkawa R, Yano K, Sato M, Ichimura N, Usami Y, Miyazaki A, Sugano M, Uehara T, Tozuka M. Paraoxonase 1 associated with high-density lipoprotein transfers to oxidized low-density lipoprotein depending on the degree of oxidation. Int J Anal Bio-Sci, 4: 99-109, 2016
  24. Sato M, *Ohkawa R, Yoshimoto A, Yano K, Ichimura N, Nishimori M, Okubo S,. Yatomi Y, Tozuka M. Effects of serum amyloid A on the structure and antioxidant ability of high-density lipoprotein. Biosci Rep 2016. doi: 10.1042/BSR20160075.
  25. Nagura Y, *Tsuno NH, Kano K, Inoue A, Aoki J, Hirowatari Y, Kaneko M, Kurano M, Matsuhashi M, Ohkawa R, Tozuka M, Yatomi Y, Okazaki H. Regulation of the lysophosphatidylserine and sphingosine 1-phosphate levels in autologous whole blood by the pre-strage leukocyte reduction. Transfus Med 2016, doi: 0.1111/tme.12326
  26. Ichimura N, Sato M, Yoshi moto A, Yano K, Ohkawa R, Kasama Takeshi, *Tozuka M, High-density lipoprotein binds to Mycobacterium avium and affects the infection of THP-1 macropage. J Lipids 2016, doi.org/10.1155/2016/4353620
  27. Shukuya K, Ogura S, Tokuhara Y, Okubo S, Yatomi Y, Tozuka M, *Shimosawa T. Novel round cells in urine sediment and their clinical implication. Clin Chem Acta 457: 142-149, 2016. doi: 10.1016/j.cca2016.04.017
  28. Nanya M, Sato M, Tanimoto K, Tozuka M, Mizutani S, *Takagi M. Dysregulation of the DNA damage response and KMT2A rearrangement in fetal liver cells. PLOS ONE 2015, doi:10.1371/journal.pone.0144540
  29. Kameda T, Ohkawa R, Yano K, Usami Y, Miyazaki A, Matsuda K, Kawasaki K, Sugano M, Kubota T, *Tozuka M. Effects of Myeloperoxidase-Induced Oxidation on Anti-Atherogenic Functions of High-Density Lipoprotein. J Lipids 2015, doi.org/10.1155/2015/592594
  30. Ohkawa R,Kurano M,Mishima Y,Nojiri T,Tokuhara Y,Kishimoto T,Nakamura K, Okubo S, Hosogaya S, Ozaki Y, Yokota H, Igarashi K, Ikeda H, Tozuka M, *Yatomi Y. Possible involvement of sphingomyelin in the regulation of the plasma sphingosine 1-phosphate level in human subjects. Clin Biochem 48: 690-697, 2015. doi: 10.1016/j.clinbiochem.2015.03.019.
  31. Iino J, Osada M, Kurano M, Kaneko M, Ohkawa R, Satoh Y, Okubo S, Ozaki Y, Tozuka M, Tsuno NH, *Yatomi Y. Platelet-derived sphingosine 1-phosphate induces migration of Jurkat cells. Lipid Health Dis 2014, doi:10.1186/1476-511X-13-150
  32. Miyazaki A, Sagae N, Usami@Y, Sato M, Kameda T, Yoshimoto A, Ishimine N, Matsuda K, Sugano M, Hara M, Honda H, *Tozuka M. N-homocysteinylation of apolipoprotein A-I impairs the proteinfs antioxidant ability but not its cholesterol efflux capacity. Biol Chem 2014, doi: 10.1515/hsz-2013-0262
  33. Matsuhashi M, *Tsuno NH, Sone S, Mishima Y, Nagura Y, Watanabe-Okochi N, Ikeda T, Kashiwase K, Fukuda S, Iriyama T, Hyodo H, Yamashita T, Kamei Y, Arai S, Minami M, Fujii T, Kurokawa M, Tozuka M, Takahashi K, Santoso S. The role of alloantibodies against human platelet antigen-15 in multiply platelet transfused patients. Transfusion 2013  doi: 10.1111/trf.12455
  34. Matsuhashi M, *Tsuno NH, Ikeda T, Mishima Y, Watanabe-Okochi N, Santoso S, Tozuka M, Takahashi K. The frequencies of SLC44A2 alleles among the Japanese population. Tissue Antigens 81: 227-228, 2013
  35. Ono Y, Kurano M, Ohkawa R, Yokota H, Igarashi K, Aoki J, Tozuka M, *Yatomi Y. Sphingosine 1-phosphate release from platelets during clot formation: close correlation between platelet count and serum sphingosine 1-phosphate concentration. Lipid Health Dis 2013, 12:20. doi:10.1186/1476-511X-12-20
  36. Usami Y, Kobayashi Y, Kameda T, Miyazaki A, Matsuda K, Sugano M, Kawasaki K, Kurihara Y, Kasama T, *Tozuka M. Identification of sites in apolipoprotein A-I susceptible to chymase and carboxypeptidase A digestion. Biosci Rep 33: 49-56, 2013
  37. Kameda T, Usami Y, Shimada S, Haraguchi G, Matsuda K, Sugano M, Kurihara Y, Isobe M, *Tozuka M. Determination of Myeloperoxidase-induced apoAI-apoAII Heterodimers in High-Density Lipoprotein. Ann Clin Lab Sci 42: 384-391, 2012
  38. Matsuhashi M, *Tsuno NH, Kawabata M, Mishima Y, Okochi N, Santoso S, Tozuka M, Takahashi K. The frequencies of human neutrophil alloantigens among the Japanese population. Tissue Antigens  80: 336-340, 2012
  39. Usami Y, Matsuda K, Sugano M, Ishimine N, Kurihara Y, Sumida T, Yamauchi K, *Tozuka M, Detection of chymase-digested C-terminally truncated  apolipoprotein A-I in normal human serum. J Immunol Methods  369: 51-58, 2011
  40. Ishimine N, Usami Y, Nogi S, Sumida T, Kurihara Y, Matsuda K, Nakamura K, Yamauchi K, Okumura N, *Tozuka M. Identification of N-homocysteinylated apolipoprotein AI in normal human serum. Ann Clin Biochem  47: 453-459, 2010