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Home  > Laboratory of Gene Expression  > Vector Info  > pENTR Series

pENTR Series

Fluorescent Protein Cassettes

pENTR vectors provide a fluorescent protein cassette for constructing a variety of Gateway Expression vectors.


pENTR-L1/L2 Series

pENTR-L1/L2 vectors provide a fluorescent protein cDNA cassette for expression of an unfused fluorescent protein.

The sequence information is available upon request to KUROYANAGI Hidehito.

pENTR-L1-ECFP-L2CyanInitiation/Termination codons
pENTR-L1-EGFP-L2GreenInitiation/Termination codons
pENTR-L1-EYFP-L2YellowInitiation/Termination codons
pENTR-L1-mRFP-L2RedInitiation/Termination codons

pENTR-L5/L2 Series

pENTR-L5/L2 vectors provide a fluorescent protein cDNA cassette for constructing expression vectors for fusion proteins with a C-terminal fluorescent protein.

The sequence information is available in Nature Protocols or upon request to KUROYANAGI Hidehito.

pENTR-L5-ECFP-L2 CyanWith Initiation codon
pENTR-L5-EGFP-L2GreenWith Initiation codon
pENTR-L5-(−1)EGFP-L2GreenNo Initiation codon
Frameshift (1-base deletion)
pENTR-L5-(+1)EGFP-L2GreenNo Initiation codon
Frameshift (1-base insertion)
pENTR-L5-Venus-L2YellowWith Initiation codon
pENTR-L5-HcRed-L2RedWith Initiation codon
pENTR-L5-mRFP-L2RedWith Initiation codon
pENTR-L5-(−1)mRFP-L2RedNo Initiation codon
Frameshift (1-base deletion)
pENTR-L5-(+1)mRFP-L2RedNo Initiation codon
Frameshift (1-base insertion)
pENTR-L5-GGS6-EGFP-L2GreenNo Initiation codon
6x(Gly-Gly-Ser) linker
pENTR-L5-(+1)GGS6-EGFP-L2GreenNo Initiation codon
6x(Gly-Gly-Ser) linker
Frameshift (1-base insertion)
pENTR-L5-(+2)GGS6-EGFP-L2GreenNo Initiation codon
6x(Gly-Gly-Ser) linker
Frameshift (2-base insertion)
pENTR-L5-GGS6-Venus-L2YellowNo Initiation codon
6x(Gly-Gly-Ser) linker
pENTR-L5-GGS6-mCherry-L2RedNo Initiation codon
6x(Gly-Gly-Ser) linker
pENTR-L5-(+1)GGS6-mCherry-L2RedNo Initiation codon
6x(Gly-Gly-Ser) linker
Frameshift (1-base insertion)
pENTR-L5-(+2)GGS6-mCherry-L2RedNo Initiation codon
6x(Gly-Gly-Ser) linker
Frameshift (2-base insertion)

pENTR-L1/R5 Series

pENTR-L1/R5 vectors provide a fluorescent protein cDNA cassette for constructing expression vectors for fusion proteins with an N-terminal fluorescent protein.

The sequence information is available upon request to KUROYANAGI Hidehito.

pENTR-L1-EGFP-R5GreenWith Initiation codon
No termination codon
pENTR-L1-mRFP-R5RedWith Initiation codon
No termination codon