Laboratory Molecular Genetics
Laboratory Molecular Genetics, Department of Moleculo-genetic Sciences,
Division of Biomedical Laboratory Sciences,
Graduate School of Allied Health Sciences, Tokyo Medical and Dental University

[Japanese Page]

Staff　

Associate Prof. Takatoshi Koyama M.D., Ph.D. 

Education　

Undergraduate Course

              Students learn how to analyze hematological disorders, hemostasis and thrombosis, etc., at cellular and biochemical levels. They then obtain skills to apply the techniques for laboratory diagnoses. Pregraduate research is also conducted based on the objectives of graduate course.

Graduate Course

The objectives of laboratory molecular genetics are understanding and analyzing health and diseases at moleculo-genetic levels. Students learn how to analyze hematological disorders, hemostasis and thrombosis, and so on, at moleculo-genetic levels. The main purpose is to develop specific laboratory methods useful for diagnosis and treatment of various disorders, transplantation, and gene therapy. This course prepares clinical-oriented international and multidisciplinary research leaders who can conduct research projects by themselves and present their achievements at domestic and international academic conferences and scientific journals. 

Research Subjects

① Analyses of Regulation of Blood Coagulation and Fibrinolysis

② Laboratory Moleculo-genetic Analyses on Hematological Disorders

③ Laboratory and Hematological Analyses on Activation of Blood Coagulation Associated with Extracorporeal Circulation, Surgery, and Organ Transplantation

④ Analyses of Defects of Intracellular Protein-transport in Protein-deficiency Disorders

⑤ Analyses of Regulation of Gene Expression by Vitamins

⑥ Analyses of Signal Transduction of Cytokine Receptors 

Publications　（a member and graduate students of our department are underlined）

  1. Ohsawa M, Koyama T, Nara N, Hirosawa S: Induction of tissue factor expression in human monocytic cells by protease inhibitors through activating activator protein-1 (AP-1) with phosphorylation of Jun-N-terminal kinase and p38. Thromb Res 2003;112:313-320.
  2. Tabuchi N, Shibamiya A, Koyama T, Fukuda T, van Oeveren W, Sunamori M: Leukocyte adsorption to the surface of the extracorporeal circuit influences its biocompatibility. Artificial Organs 2003;27:591-594.
  3. Nakazawa F, Koyama T, Shibamiya A, Hirosawa S: Characterization of thrombomodulin gene mutations of the 5'-regulatory region. Atherosclerosis 2002; 164:385-387.
  4. Yoshinaga H, Nakahara M, Koyama T, Shibamiya A, Nakazawa F, Miles LA, Hirosawa S, Aoki N: A single thymine nucleotide deletion responsible for congenital deficiency of plasmin inhibitor. Thromb Haemost 2002; 88:144-148.
  5. Suzuki K, Koyama T, Kobayashi S, Kobayashi K, Inagaki K, Abe Y, Kuriyama K, Shiba K: Novel method for detection of ex vivo tumor necrosis factor a production by monocytes.J Clin Lab Anal 2002; 16:273-278.
  6. Koyama T, Ohsawa M, Nakazawa F, Shibamiya A: Anticoagulant effects of synthetic retinoids and activated vitamin D3(1,25(OH)2D3) analogs. Modern Trends in Skin Pharmacology: Molecular and Clinical Aspects, 189-215, edited by Tsambaos D & Merk H. Parissianos Medical Publishers, Athens, Greece, 2002.
  7. Madoiwa S, Nakamura Y, Mimuro J, Furusawa S, Koyama T, Sugo T, Matsuda M, Sakata Y: Autoantibody against prothrombin aberrantly alters the proenzyme to facilitate formation of a complex with its physiological inhibitor antithrombin III without thrombin conversion. Blood 2001;97:3783-3789.
  8. Tabuchi N, Koyama T, Shibamiya A, Sunamori M: Remaining procoagulant property of wound blood washed by a cell-saving device. Ann Thorac Surg 2001;71:1749.
  9. Matsuda K, Hiratsuka N, Koyama T, Kurihara Y, Hotta O, Itoh Y, Shiba K: S ensitive method for detection and semiquantification of Bence Jones protein by cellulose acetate membrane electrophoresis using colloidal silver staining. Clin Chem 2001;47: 763-766.
  10. Ohsawa M, Koyama T, Yamamoto K, Hirosawa S, Kamei S, Kamiyama R: 1 a ,25-dihydroxyvitamin D3 and its potent synthetic analogs downregulate tissue factor and upregulate thrombomodulin expression in monocytic cells, counteracting the effects of tumor necrosis factor and oxidized LDL. Circulation 2000;102:2867-2872.
  11. Kikuchi M, Miki T, Kumagai T, Fukuda T, Kamiyama R, Miyasaka N, Hirosawa S: Identification of negative regulatory regions within the first exon and intron of the BCL6 gene. Oncogene 2000;19:4941-4945.
  12. Ohsawa M, Koyama T, Shibakura M, Kamei S, Hirosawa S: Arsenic trioxide (As2O3) gradually downregulates tissue factor expression without affecting thrombomodulin expression in acute promyelocytic leukemia cells. Leukemia 2000;14:941-943.
  13. Yamamoto K, Shibata F, Miura O, Kamiyama R, Hirosawa S, Miyasaka N: Physical interaction between interleukin-12 receptor b 2 subunit and Jak2  tyrosine kinase: Jak2 associates with cytoplasmic membrane-proximal region of interleukin-12 receptor b 2 via amino-terminus. Biochem Biophys Res Comm 1999;257:400-404.
  14. Nakazawa F, Koyama T, Saito T, Shibakura M, Yoshinaga H, Chung DH, Kamiyama R, Hirosawa S: Thrombomodulin with the Asp468Tyr mutation is expressed on the cell surface with normal cofactor activity for protein C activation. Br J Haematol 1999;106:416-420.
  15. Kumagai T, Miki T, Kikuchi M, Fukuda T, Miyasaka N, Kamiyama R, Hirosawa S: The proto-oncogene Bcl6 inhibits apoptotic cell death in differentiation-induced mouse myogenic cells. Oncogene 1999;18:467-475.
  16. Shibakura M, Koyama T, Ohsawa M, Kamiyama R, Hirosawa S: A retinoic acid receptor- a (RAR a ) selective agonist modulates procoagulant activity of acute promyelocytic cells and induces their differentiation into neutrophils. Blood 1998;91:724-725.
  17. Chin H, Arai A, Wakao H, Kamiyama R, Miyasaka N, Miura O: Lyn physically associates with the erythropoietin receptor and may play a role in activation of the Stat5 pathway. Blood 1998;91:3734-3745.
  18. Koyama T, Shibakura M, Ohsawa M, Kamiyama R, Hirosawa S: Anticoagulant effects of 1 a ,25-dihydroxyvitamin D3on human myelogenous leukemia cells and monocytes. Blood 1998;92:160-167.
  19. Sakasita C, Saito T, Kurosu T, Yoshinaga H, Kumagai T, Yamamoto K, Miki T, Koyama T, Miura O, Nemoto T, Asakawa H, Hirosawa S: Two M-components in a single cell lineage in a patient with a dual isotype secretory B-cell tumor. Br J Haematol 1998;102:791-794.
  20. Koyama T, Hirosawa S: Anticoagulant effects of synthetic retinoids and activated vitamin D3. Semin Thromb Hemost 1998;24:217-226.
  21. Koyama T, Shibakura M: Anticoagulant effects of synthetic retinoids. Leukemia and Lymphoma 1998;31:71-80.
  22. Koyama T, Aoki N: a 2 -Makroglobulin und Protease Nexin 1: Struktur und Funktion Hamostaselogie, 413-415, edited by Muller-Berghaus G & Potzsch B, Springer-Verlag, Heidelberg, Germany, 1998
  23. Chin H, Wakao H, Miyajima A, Kamiyama R, Miyasaka N, Miura O: Erythropoietin induces tyrosine phosphorylation of the interleukin-3 receptor b subunit ( b IL3) and recruitment of stat5 to possible stat5-docking sites in bIL3. Blood 1997;89:4327-4336.
  24. Chin H, Saito T, Arai A, Yamamoto K, Kamiyama R, Miyasaka N, Miura O: Erythropoietin and IL-3 induce tyrosine phosphorylation of CrkL and its association with Shc, SHP-2, and Cbl in hematopoietic cells. Biochem Biophys Res Comm 1997;239:412-417.
  25. Shibakura M, Koyama T, Saito T, Shudo K, Miyasaka N, Kamiyama R, Hirosawa S: Anticoagulant effects of synthetic retinoids mediated via different receptors on human leukemia and umbilical vein endothelial cells. Blood 1997;90:1545-1551.
  26. Koyama T, Aoki N: Plasma tissue factor reflects endothelial cell injury rather than upregulation of tissue factor expression. Thromb Haemost 1997;78:972.
  27. Qi H, Koyama T, Nishida K, Miyasaka N, Hirosawa S: Urinary procoagulant activity and tissue factor levels in patients with diabetes mellitus. Haemostasis 1997;27:57-64.
  28. Saito T, Koyama T, Nagata K, Kamiyama R, Hirosawa S: Anticoagulant effects of retinoic acids on leukemia cells. Blood 1996;87:657-665.
  29. Yoshinaga H, Ohashi K, Yamamoto K, Miki T, Miura O, Saito T, Koyama T, Kanazawa A, Nemoto T, Miyasaka N, Kamiyama R, Hirosawa S: Clonal identification of Burkitt's lymphoma arising from lymphocyte-predominant Hodgkin's disease. Br J Haematol 1996;95:380-382.
  30. Koyama T, Aoki N: Plasminogen alteration. Hypercoagulable States; Fundamental Aspects, Acquired Disorders and Congenital Thrombophilia, 389-394, edited by Seghatchian MJ, Samama MM & Hecker SP, CRC-Press, Boca Raton, Florida, 1996.
  31. Koyama T, Saito T, Kusano T, Hirosawa S: Factor V inhibitor associated with Sjogren's syndrome. Br J Haematol 1995;89:893-896.
  32. Koyama T, Koike Y, Toyota S, Miyagi F, Suzuki N, Aoki N: Different NH2-terminal form with 12 additional residues of a2-plasmin inhibitor from human plasma and culture media of HepG2 cells. Biochem Biophys Res Commun 1994;200:417-422.
  33. Koyama T, Nishida K, Sawada M, Ohdama S, Murakami N, Hirosawa S, Kuriyama R, Matsuzawa K, Hasegawa R, Aoki N: Determination of plasma tissue factor antigen and its clinical significance. Br J Haematol 1994;87:343-347.
  34. Koyama T, Hirosawa S, Kawamata N, Tohda S, Aoki N: All-trans retinoic acid (ATRA) upregulates thrombomodulin and downregulates tissue factor expression in acute promyelocytic leukemia cells: Distinct expression of thrombomodulin and tissue factor in human leukemic cells. Blood 1994;84:3001-3009.  

[Graduate School of Allied Health Sciences]
[Tokyo Medical and Dental University]

1-5-45, Yushima, Bunkyo-ku, Tokyo 113-8519, Japan
TEL, FAX: +81-3-5803-5882

Last Updated : 1 August 2003